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Immunofluorescent staining of cancer spheroids and fine-needle aspiration-derived organoids

Our organoid generation technique has allowed for the development of downstream organoid applications. Here, we detail an accessible, straightforward protocol for immunofluorescent staining and imaging of thyroid cancer organoids, particularly those with tumor de-differentiation. Immunofluorescence...

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Detalles Bibliográficos
Autores principales: Bergdorf, Kensey N., Phifer, Courtney J., Bechard, Matthew E., Lee, Mason A., McDonald, Oliver G., Lee, Ethan, Weiss, Vivian L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8182106/
https://www.ncbi.nlm.nih.gov/pubmed/34136836
http://dx.doi.org/10.1016/j.xpro.2021.100578