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Detection of feline norovirus using commercial real-time RT-PCR kit for the diagnosis of human norovirus infection
Feline noroviruses (FNoVs) are potential clinical pathogens in cats. To perform an epidemiological study of FNoV infection, it is necessary to develop a simple and effective method for virus detection. We investigated whether a commercial human NoV quantitative RT-PCR kit for the detection of human...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Japanese Society of Veterinary Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8182310/ https://www.ncbi.nlm.nih.gov/pubmed/33692232 http://dx.doi.org/10.1292/jvms.20-0703 |
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author | TAKANO, Tomomi WATANABE, Haruna DOKI, Tomoyoshi KUSUHARA, Hajime |
author_facet | TAKANO, Tomomi WATANABE, Haruna DOKI, Tomoyoshi KUSUHARA, Hajime |
author_sort | TAKANO, Tomomi |
collection | PubMed |
description | Feline noroviruses (FNoVs) are potential clinical pathogens in cats. To perform an epidemiological study of FNoV infection, it is necessary to develop a simple and effective method for virus detection. We investigated whether a commercial human NoV quantitative RT-PCR kit for the detection of human NoVs used in medical practice can be applied for FNoV detection. This kit was capable of detecting the FNoV gene regardless of the genogroup (GIV and GVI) in experimental and field samples. Based on the above findings, it is possible to detect FNoVs using human NoV tests. The relationship between FNoV infection and gastroenteritis in cats may be clarified by applying these methods to an epidemiological survey of FNoVs. |
format | Online Article Text |
id | pubmed-8182310 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | The Japanese Society of Veterinary Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-81823102021-06-09 Detection of feline norovirus using commercial real-time RT-PCR kit for the diagnosis of human norovirus infection TAKANO, Tomomi WATANABE, Haruna DOKI, Tomoyoshi KUSUHARA, Hajime J Vet Med Sci Virology Feline noroviruses (FNoVs) are potential clinical pathogens in cats. To perform an epidemiological study of FNoV infection, it is necessary to develop a simple and effective method for virus detection. We investigated whether a commercial human NoV quantitative RT-PCR kit for the detection of human NoVs used in medical practice can be applied for FNoV detection. This kit was capable of detecting the FNoV gene regardless of the genogroup (GIV and GVI) in experimental and field samples. Based on the above findings, it is possible to detect FNoVs using human NoV tests. The relationship between FNoV infection and gastroenteritis in cats may be clarified by applying these methods to an epidemiological survey of FNoVs. The Japanese Society of Veterinary Science 2021-03-11 2021-05 /pmc/articles/PMC8182310/ /pubmed/33692232 http://dx.doi.org/10.1292/jvms.20-0703 Text en ©2021 The Japanese Society of Veterinary Science https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/) |
spellingShingle | Virology TAKANO, Tomomi WATANABE, Haruna DOKI, Tomoyoshi KUSUHARA, Hajime Detection of feline norovirus using commercial real-time RT-PCR kit for the diagnosis of human norovirus infection |
title | Detection of feline norovirus using commercial real-time RT-PCR kit for the diagnosis of human norovirus infection |
title_full | Detection of feline norovirus using commercial real-time RT-PCR kit for the diagnosis of human norovirus infection |
title_fullStr | Detection of feline norovirus using commercial real-time RT-PCR kit for the diagnosis of human norovirus infection |
title_full_unstemmed | Detection of feline norovirus using commercial real-time RT-PCR kit for the diagnosis of human norovirus infection |
title_short | Detection of feline norovirus using commercial real-time RT-PCR kit for the diagnosis of human norovirus infection |
title_sort | detection of feline norovirus using commercial real-time rt-pcr kit for the diagnosis of human norovirus infection |
topic | Virology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8182310/ https://www.ncbi.nlm.nih.gov/pubmed/33692232 http://dx.doi.org/10.1292/jvms.20-0703 |
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