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Detection of feline norovirus using commercial real-time RT-PCR kit for the diagnosis of human norovirus infection

Feline noroviruses (FNoVs) are potential clinical pathogens in cats. To perform an epidemiological study of FNoV infection, it is necessary to develop a simple and effective method for virus detection. We investigated whether a commercial human NoV quantitative RT-PCR kit for the detection of human...

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Autores principales: TAKANO, Tomomi, WATANABE, Haruna, DOKI, Tomoyoshi, KUSUHARA, Hajime
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8182310/
https://www.ncbi.nlm.nih.gov/pubmed/33692232
http://dx.doi.org/10.1292/jvms.20-0703
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author TAKANO, Tomomi
WATANABE, Haruna
DOKI, Tomoyoshi
KUSUHARA, Hajime
author_facet TAKANO, Tomomi
WATANABE, Haruna
DOKI, Tomoyoshi
KUSUHARA, Hajime
author_sort TAKANO, Tomomi
collection PubMed
description Feline noroviruses (FNoVs) are potential clinical pathogens in cats. To perform an epidemiological study of FNoV infection, it is necessary to develop a simple and effective method for virus detection. We investigated whether a commercial human NoV quantitative RT-PCR kit for the detection of human NoVs used in medical practice can be applied for FNoV detection. This kit was capable of detecting the FNoV gene regardless of the genogroup (GIV and GVI) in experimental and field samples. Based on the above findings, it is possible to detect FNoVs using human NoV tests. The relationship between FNoV infection and gastroenteritis in cats may be clarified by applying these methods to an epidemiological survey of FNoVs.
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spelling pubmed-81823102021-06-09 Detection of feline norovirus using commercial real-time RT-PCR kit for the diagnosis of human norovirus infection TAKANO, Tomomi WATANABE, Haruna DOKI, Tomoyoshi KUSUHARA, Hajime J Vet Med Sci Virology Feline noroviruses (FNoVs) are potential clinical pathogens in cats. To perform an epidemiological study of FNoV infection, it is necessary to develop a simple and effective method for virus detection. We investigated whether a commercial human NoV quantitative RT-PCR kit for the detection of human NoVs used in medical practice can be applied for FNoV detection. This kit was capable of detecting the FNoV gene regardless of the genogroup (GIV and GVI) in experimental and field samples. Based on the above findings, it is possible to detect FNoVs using human NoV tests. The relationship between FNoV infection and gastroenteritis in cats may be clarified by applying these methods to an epidemiological survey of FNoVs. The Japanese Society of Veterinary Science 2021-03-11 2021-05 /pmc/articles/PMC8182310/ /pubmed/33692232 http://dx.doi.org/10.1292/jvms.20-0703 Text en ©2021 The Japanese Society of Veterinary Science https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Virology
TAKANO, Tomomi
WATANABE, Haruna
DOKI, Tomoyoshi
KUSUHARA, Hajime
Detection of feline norovirus using commercial real-time RT-PCR kit for the diagnosis of human norovirus infection
title Detection of feline norovirus using commercial real-time RT-PCR kit for the diagnosis of human norovirus infection
title_full Detection of feline norovirus using commercial real-time RT-PCR kit for the diagnosis of human norovirus infection
title_fullStr Detection of feline norovirus using commercial real-time RT-PCR kit for the diagnosis of human norovirus infection
title_full_unstemmed Detection of feline norovirus using commercial real-time RT-PCR kit for the diagnosis of human norovirus infection
title_short Detection of feline norovirus using commercial real-time RT-PCR kit for the diagnosis of human norovirus infection
title_sort detection of feline norovirus using commercial real-time rt-pcr kit for the diagnosis of human norovirus infection
topic Virology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8182310/
https://www.ncbi.nlm.nih.gov/pubmed/33692232
http://dx.doi.org/10.1292/jvms.20-0703
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