A rapid multiple immunofluorescence method for the simultaneous detection of CD20 and CD3 in canine and feline cytological samples

CD20 and CD3 are considered reliable markers for B and T cells, respectively. This study aimed to develop a rapid multiple immunofluorescence (RMIF) method for the detection of CD20 and CD3 on a single cytology slide. Air-dried smears were prepared using samples collected from dogs (n=26) and cats (...

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Autores principales: MATSUMOTO, Fumi, SHIMA-SAWA, Mariko, FURUSAWA, Yu, YAMATO, Osamu, YABUKI, Akira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2021
Materias:
Internal Medicine
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8182324/
https://www.ncbi.nlm.nih.gov/pubmed/33814519
http://dx.doi.org/10.1292/jvms.20-0597
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author MATSUMOTO, Fumi
SHIMA-SAWA, Mariko
FURUSAWA, Yu
YAMATO, Osamu
YABUKI, Akira
author_facet MATSUMOTO, Fumi
SHIMA-SAWA, Mariko
FURUSAWA, Yu
YAMATO, Osamu
YABUKI, Akira
author_sort MATSUMOTO, Fumi
collection PubMed
description CD20 and CD3 are considered reliable markers for B and T cells, respectively. This study aimed to develop a rapid multiple immunofluorescence (RMIF) method for the detection of CD20 and CD3 on a single cytology slide. Air-dried smears were prepared using samples collected from dogs (n=26) and cats (n=6). Immunosignal detection using the newly developed method required 60 min. Clear immunosignals for CD20 and CD3 were detected in 24 of 26 samples in dogs and in all 6 cats. As the RMIF (CD20/CD3) method can detect markers of both B and T cells simultaneously on a single cytology smear, it would be an efficient tool for the immunophenotyping of canine and feline lymphoma samples.
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spelling pubmed-81823242021-06-09 A rapid multiple immunofluorescence method for the simultaneous detection of CD20 and CD3 in canine and feline cytological samples MATSUMOTO, Fumi SHIMA-SAWA, Mariko FURUSAWA, Yu YAMATO, Osamu YABUKI, Akira J Vet Med Sci Internal Medicine CD20 and CD3 are considered reliable markers for B and T cells, respectively. This study aimed to develop a rapid multiple immunofluorescence (RMIF) method for the detection of CD20 and CD3 on a single cytology slide. Air-dried smears were prepared using samples collected from dogs (n=26) and cats (n=6). Immunosignal detection using the newly developed method required 60 min. Clear immunosignals for CD20 and CD3 were detected in 24 of 26 samples in dogs and in all 6 cats. As the RMIF (CD20/CD3) method can detect markers of both B and T cells simultaneously on a single cytology smear, it would be an efficient tool for the immunophenotyping of canine and feline lymphoma samples. The Japanese Society of Veterinary Science 2021-04-05 2021-05 /pmc/articles/PMC8182324/ /pubmed/33814519 http://dx.doi.org/10.1292/jvms.20-0597 Text en ©2021 The Japanese Society of Veterinary Science https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Internal Medicine
MATSUMOTO, Fumi
SHIMA-SAWA, Mariko
FURUSAWA, Yu
YAMATO, Osamu
YABUKI, Akira
A rapid multiple immunofluorescence method for the simultaneous detection of CD20 and CD3 in canine and feline cytological samples
title A rapid multiple immunofluorescence method for the simultaneous detection of CD20 and CD3 in canine and feline cytological samples
title_full A rapid multiple immunofluorescence method for the simultaneous detection of CD20 and CD3 in canine and feline cytological samples
title_fullStr A rapid multiple immunofluorescence method for the simultaneous detection of CD20 and CD3 in canine and feline cytological samples
title_full_unstemmed A rapid multiple immunofluorescence method for the simultaneous detection of CD20 and CD3 in canine and feline cytological samples
title_short A rapid multiple immunofluorescence method for the simultaneous detection of CD20 and CD3 in canine and feline cytological samples
title_sort rapid multiple immunofluorescence method for the simultaneous detection of cd20 and cd3 in canine and feline cytological samples
topic Internal Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8182324/
https://www.ncbi.nlm.nih.gov/pubmed/33814519
http://dx.doi.org/10.1292/jvms.20-0597
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