Cargando…

Knockdown of lncRNA TUC338 inhibits esophageal cancer cells migration and invasion

BACKGROUND: Long non-coding RNAs (lncRNAs) are firmly identified with the event and improvement of tumors. Therefore, elucidating the functions and mechanisms of related lncRNAs is significant for understanding the occurrence and advancement of tumors. The recently discovered lncRNA TUC338 has been...

Descripción completa

Detalles Bibliográficos
Autores principales: Qian, Ting, Zhang, Hui, Yu, Shaorong, Chen, Zhenzhang, Jia, Hui, Peng, Fanyu, Cao, Guochun, Lu, Jianwei, Liu, Delin, Sun, Dawei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AME Publishing Company 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8182530/
https://www.ncbi.nlm.nih.gov/pubmed/34164197
http://dx.doi.org/10.21037/jtd-21-563
_version_ 1783704225829617664
author Qian, Ting
Zhang, Hui
Yu, Shaorong
Chen, Zhenzhang
Jia, Hui
Peng, Fanyu
Cao, Guochun
Lu, Jianwei
Liu, Delin
Sun, Dawei
author_facet Qian, Ting
Zhang, Hui
Yu, Shaorong
Chen, Zhenzhang
Jia, Hui
Peng, Fanyu
Cao, Guochun
Lu, Jianwei
Liu, Delin
Sun, Dawei
author_sort Qian, Ting
collection PubMed
description BACKGROUND: Long non-coding RNAs (lncRNAs) are firmly identified with the event and improvement of tumors. Therefore, elucidating the functions and mechanisms of related lncRNAs is significant for understanding the occurrence and advancement of tumors. The recently discovered lncRNA TUC338 has been shown to play the role of an oncogene in an assortment of tumors. Be that as it may, the articulation and elements of lncRNA TUC338 in esophageal cancer are as yet hazy. This investigation plans to explain the capacities and related molecular mechanisms of lncRNA TUC338 in esophageal malignancy. METHODS: Firstly, the expression of TUC338 in 50 instances of esophageal disease tissues and nearby tissues was detected by fluorescence reckonable PCR, and correlations with the clinic pathological characteristics of patients was further analyzed. Then, a lentiviral interference vector was designed and transfected into an esophageal cancer cell line, and knockdown was verified by fluorescence quantitative PCR. The effects of TUC338 knockdown on the proliferation, clone formation, and migration and infringement of esophageal malignancy cells were tested utilizing the CCK-8 assay, clone formation experiments, and Transwell experiments. Western blot detected the expression of invasion-related proteins. RESULTS: Fluorescence reckonable PCR exhibit that TUC338 was exceptionally communicated in esophageal cancer tissues, and was significantly related with metastasis and TNM stage in tolerant. Functional experiments showed that in esophageal disease cell lines, knocking down the declaration of TUC338 significantly inhibited cell multiplication, clone development, and intrusion and movement. Further experiments on molecular mechanisms showed that knocking down TUC338 inhibited statement of N-cadherin and vimentin in cells. CONCLUSIONS: TUC338 is exceptionally communicated in esophageal malignancy tissues and can regulate cell proliferation and invasion.
format Online
Article
Text
id pubmed-8182530
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher AME Publishing Company
record_format MEDLINE/PubMed
spelling pubmed-81825302021-06-22 Knockdown of lncRNA TUC338 inhibits esophageal cancer cells migration and invasion Qian, Ting Zhang, Hui Yu, Shaorong Chen, Zhenzhang Jia, Hui Peng, Fanyu Cao, Guochun Lu, Jianwei Liu, Delin Sun, Dawei J Thorac Dis Original Article BACKGROUND: Long non-coding RNAs (lncRNAs) are firmly identified with the event and improvement of tumors. Therefore, elucidating the functions and mechanisms of related lncRNAs is significant for understanding the occurrence and advancement of tumors. The recently discovered lncRNA TUC338 has been shown to play the role of an oncogene in an assortment of tumors. Be that as it may, the articulation and elements of lncRNA TUC338 in esophageal cancer are as yet hazy. This investigation plans to explain the capacities and related molecular mechanisms of lncRNA TUC338 in esophageal malignancy. METHODS: Firstly, the expression of TUC338 in 50 instances of esophageal disease tissues and nearby tissues was detected by fluorescence reckonable PCR, and correlations with the clinic pathological characteristics of patients was further analyzed. Then, a lentiviral interference vector was designed and transfected into an esophageal cancer cell line, and knockdown was verified by fluorescence quantitative PCR. The effects of TUC338 knockdown on the proliferation, clone formation, and migration and infringement of esophageal malignancy cells were tested utilizing the CCK-8 assay, clone formation experiments, and Transwell experiments. Western blot detected the expression of invasion-related proteins. RESULTS: Fluorescence reckonable PCR exhibit that TUC338 was exceptionally communicated in esophageal cancer tissues, and was significantly related with metastasis and TNM stage in tolerant. Functional experiments showed that in esophageal disease cell lines, knocking down the declaration of TUC338 significantly inhibited cell multiplication, clone development, and intrusion and movement. Further experiments on molecular mechanisms showed that knocking down TUC338 inhibited statement of N-cadherin and vimentin in cells. CONCLUSIONS: TUC338 is exceptionally communicated in esophageal malignancy tissues and can regulate cell proliferation and invasion. AME Publishing Company 2021-05 /pmc/articles/PMC8182530/ /pubmed/34164197 http://dx.doi.org/10.21037/jtd-21-563 Text en 2021 Journal of Thoracic Disease. All rights reserved. https://creativecommons.org/licenses/by-nc-nd/4.0/Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Original Article
Qian, Ting
Zhang, Hui
Yu, Shaorong
Chen, Zhenzhang
Jia, Hui
Peng, Fanyu
Cao, Guochun
Lu, Jianwei
Liu, Delin
Sun, Dawei
Knockdown of lncRNA TUC338 inhibits esophageal cancer cells migration and invasion
title Knockdown of lncRNA TUC338 inhibits esophageal cancer cells migration and invasion
title_full Knockdown of lncRNA TUC338 inhibits esophageal cancer cells migration and invasion
title_fullStr Knockdown of lncRNA TUC338 inhibits esophageal cancer cells migration and invasion
title_full_unstemmed Knockdown of lncRNA TUC338 inhibits esophageal cancer cells migration and invasion
title_short Knockdown of lncRNA TUC338 inhibits esophageal cancer cells migration and invasion
title_sort knockdown of lncrna tuc338 inhibits esophageal cancer cells migration and invasion
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8182530/
https://www.ncbi.nlm.nih.gov/pubmed/34164197
http://dx.doi.org/10.21037/jtd-21-563
work_keys_str_mv AT qianting knockdownoflncrnatuc338inhibitsesophagealcancercellsmigrationandinvasion
AT zhanghui knockdownoflncrnatuc338inhibitsesophagealcancercellsmigrationandinvasion
AT yushaorong knockdownoflncrnatuc338inhibitsesophagealcancercellsmigrationandinvasion
AT chenzhenzhang knockdownoflncrnatuc338inhibitsesophagealcancercellsmigrationandinvasion
AT jiahui knockdownoflncrnatuc338inhibitsesophagealcancercellsmigrationandinvasion
AT pengfanyu knockdownoflncrnatuc338inhibitsesophagealcancercellsmigrationandinvasion
AT caoguochun knockdownoflncrnatuc338inhibitsesophagealcancercellsmigrationandinvasion
AT lujianwei knockdownoflncrnatuc338inhibitsesophagealcancercellsmigrationandinvasion
AT liudelin knockdownoflncrnatuc338inhibitsesophagealcancercellsmigrationandinvasion
AT sundawei knockdownoflncrnatuc338inhibitsesophagealcancercellsmigrationandinvasion