Cargando…
Knockdown of lncRNA TUC338 inhibits esophageal cancer cells migration and invasion
BACKGROUND: Long non-coding RNAs (lncRNAs) are firmly identified with the event and improvement of tumors. Therefore, elucidating the functions and mechanisms of related lncRNAs is significant for understanding the occurrence and advancement of tumors. The recently discovered lncRNA TUC338 has been...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
AME Publishing Company
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8182530/ https://www.ncbi.nlm.nih.gov/pubmed/34164197 http://dx.doi.org/10.21037/jtd-21-563 |
_version_ | 1783704225829617664 |
---|---|
author | Qian, Ting Zhang, Hui Yu, Shaorong Chen, Zhenzhang Jia, Hui Peng, Fanyu Cao, Guochun Lu, Jianwei Liu, Delin Sun, Dawei |
author_facet | Qian, Ting Zhang, Hui Yu, Shaorong Chen, Zhenzhang Jia, Hui Peng, Fanyu Cao, Guochun Lu, Jianwei Liu, Delin Sun, Dawei |
author_sort | Qian, Ting |
collection | PubMed |
description | BACKGROUND: Long non-coding RNAs (lncRNAs) are firmly identified with the event and improvement of tumors. Therefore, elucidating the functions and mechanisms of related lncRNAs is significant for understanding the occurrence and advancement of tumors. The recently discovered lncRNA TUC338 has been shown to play the role of an oncogene in an assortment of tumors. Be that as it may, the articulation and elements of lncRNA TUC338 in esophageal cancer are as yet hazy. This investigation plans to explain the capacities and related molecular mechanisms of lncRNA TUC338 in esophageal malignancy. METHODS: Firstly, the expression of TUC338 in 50 instances of esophageal disease tissues and nearby tissues was detected by fluorescence reckonable PCR, and correlations with the clinic pathological characteristics of patients was further analyzed. Then, a lentiviral interference vector was designed and transfected into an esophageal cancer cell line, and knockdown was verified by fluorescence quantitative PCR. The effects of TUC338 knockdown on the proliferation, clone formation, and migration and infringement of esophageal malignancy cells were tested utilizing the CCK-8 assay, clone formation experiments, and Transwell experiments. Western blot detected the expression of invasion-related proteins. RESULTS: Fluorescence reckonable PCR exhibit that TUC338 was exceptionally communicated in esophageal cancer tissues, and was significantly related with metastasis and TNM stage in tolerant. Functional experiments showed that in esophageal disease cell lines, knocking down the declaration of TUC338 significantly inhibited cell multiplication, clone development, and intrusion and movement. Further experiments on molecular mechanisms showed that knocking down TUC338 inhibited statement of N-cadherin and vimentin in cells. CONCLUSIONS: TUC338 is exceptionally communicated in esophageal malignancy tissues and can regulate cell proliferation and invasion. |
format | Online Article Text |
id | pubmed-8182530 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | AME Publishing Company |
record_format | MEDLINE/PubMed |
spelling | pubmed-81825302021-06-22 Knockdown of lncRNA TUC338 inhibits esophageal cancer cells migration and invasion Qian, Ting Zhang, Hui Yu, Shaorong Chen, Zhenzhang Jia, Hui Peng, Fanyu Cao, Guochun Lu, Jianwei Liu, Delin Sun, Dawei J Thorac Dis Original Article BACKGROUND: Long non-coding RNAs (lncRNAs) are firmly identified with the event and improvement of tumors. Therefore, elucidating the functions and mechanisms of related lncRNAs is significant for understanding the occurrence and advancement of tumors. The recently discovered lncRNA TUC338 has been shown to play the role of an oncogene in an assortment of tumors. Be that as it may, the articulation and elements of lncRNA TUC338 in esophageal cancer are as yet hazy. This investigation plans to explain the capacities and related molecular mechanisms of lncRNA TUC338 in esophageal malignancy. METHODS: Firstly, the expression of TUC338 in 50 instances of esophageal disease tissues and nearby tissues was detected by fluorescence reckonable PCR, and correlations with the clinic pathological characteristics of patients was further analyzed. Then, a lentiviral interference vector was designed and transfected into an esophageal cancer cell line, and knockdown was verified by fluorescence quantitative PCR. The effects of TUC338 knockdown on the proliferation, clone formation, and migration and infringement of esophageal malignancy cells were tested utilizing the CCK-8 assay, clone formation experiments, and Transwell experiments. Western blot detected the expression of invasion-related proteins. RESULTS: Fluorescence reckonable PCR exhibit that TUC338 was exceptionally communicated in esophageal cancer tissues, and was significantly related with metastasis and TNM stage in tolerant. Functional experiments showed that in esophageal disease cell lines, knocking down the declaration of TUC338 significantly inhibited cell multiplication, clone development, and intrusion and movement. Further experiments on molecular mechanisms showed that knocking down TUC338 inhibited statement of N-cadherin and vimentin in cells. CONCLUSIONS: TUC338 is exceptionally communicated in esophageal malignancy tissues and can regulate cell proliferation and invasion. AME Publishing Company 2021-05 /pmc/articles/PMC8182530/ /pubmed/34164197 http://dx.doi.org/10.21037/jtd-21-563 Text en 2021 Journal of Thoracic Disease. All rights reserved. https://creativecommons.org/licenses/by-nc-nd/4.0/Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) . |
spellingShingle | Original Article Qian, Ting Zhang, Hui Yu, Shaorong Chen, Zhenzhang Jia, Hui Peng, Fanyu Cao, Guochun Lu, Jianwei Liu, Delin Sun, Dawei Knockdown of lncRNA TUC338 inhibits esophageal cancer cells migration and invasion |
title | Knockdown of lncRNA TUC338 inhibits esophageal cancer cells migration and invasion |
title_full | Knockdown of lncRNA TUC338 inhibits esophageal cancer cells migration and invasion |
title_fullStr | Knockdown of lncRNA TUC338 inhibits esophageal cancer cells migration and invasion |
title_full_unstemmed | Knockdown of lncRNA TUC338 inhibits esophageal cancer cells migration and invasion |
title_short | Knockdown of lncRNA TUC338 inhibits esophageal cancer cells migration and invasion |
title_sort | knockdown of lncrna tuc338 inhibits esophageal cancer cells migration and invasion |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8182530/ https://www.ncbi.nlm.nih.gov/pubmed/34164197 http://dx.doi.org/10.21037/jtd-21-563 |
work_keys_str_mv | AT qianting knockdownoflncrnatuc338inhibitsesophagealcancercellsmigrationandinvasion AT zhanghui knockdownoflncrnatuc338inhibitsesophagealcancercellsmigrationandinvasion AT yushaorong knockdownoflncrnatuc338inhibitsesophagealcancercellsmigrationandinvasion AT chenzhenzhang knockdownoflncrnatuc338inhibitsesophagealcancercellsmigrationandinvasion AT jiahui knockdownoflncrnatuc338inhibitsesophagealcancercellsmigrationandinvasion AT pengfanyu knockdownoflncrnatuc338inhibitsesophagealcancercellsmigrationandinvasion AT caoguochun knockdownoflncrnatuc338inhibitsesophagealcancercellsmigrationandinvasion AT lujianwei knockdownoflncrnatuc338inhibitsesophagealcancercellsmigrationandinvasion AT liudelin knockdownoflncrnatuc338inhibitsesophagealcancercellsmigrationandinvasion AT sundawei knockdownoflncrnatuc338inhibitsesophagealcancercellsmigrationandinvasion |