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Interaction between LINC-ROR and Stemness State in Gastric Cancer Cells with Helicobacter pylori Infection

BACKGROUND: LINC-ROR, as a cancer-related lncRNA, has vital roles in stem cell survival, pluripotency, differentiation, and self-renewal in hESCs. However, cancer-related molecular mechanisms, its functional roles, and clinical value of LINC-ROR in GC remain unclear. In this study, we aimed to inves...

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Autores principales: Alsadat Mahmoudian, Reihaneh, Lotfi Gharaie, Maryam, Abbaszadegan, Roya, Forghanifard, Mohammad Mahdi, Abbaszadegan, Mohammad Reza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pasteur Institute of Iran 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8183384/
https://www.ncbi.nlm.nih.gov/pubmed/33745265
http://dx.doi.org/10.52547/ibj.25.3.157
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author Alsadat Mahmoudian, Reihaneh
Lotfi Gharaie, Maryam
Abbaszadegan, Roya
Forghanifard, Mohammad Mahdi
Abbaszadegan, Mohammad Reza
author_facet Alsadat Mahmoudian, Reihaneh
Lotfi Gharaie, Maryam
Abbaszadegan, Roya
Forghanifard, Mohammad Mahdi
Abbaszadegan, Mohammad Reza
author_sort Alsadat Mahmoudian, Reihaneh
collection PubMed
description BACKGROUND: LINC-ROR, as a cancer-related lncRNA, has vital roles in stem cell survival, pluripotency, differentiation, and self-renewal in hESCs. However, cancer-related molecular mechanisms, its functional roles, and clinical value of LINC-ROR in GC remain unclear. In this study, we aimed to investigate probable interplay between LINC-ROR with SALL4 stemness regulator and their role with the development of the disease. METHODS: The mRNA expression profile of LINC-ROR and SALL4 was assessed in tumoral and adjacent non-cancerous tissues of GC patients, using quantitative real-time PCR. RESULTS: Significant LINC-ROR underexpression and SALL4 overexpression were observed in 55.81% and 75.58% (p < 0.0001) of samples, respectively. The expression of LINC-ROR and SALL4 were significantly correlated with each other (p = 0.044). There was an association between the underexpression of LINC-ROR and sex, stage of tumor progression, tumor type, and location of tumor (p < 0.05), and H. pylori infection with SALL4 expression (p = 0.036). There were also significant correlations between concomitant mRNA expression of SALL4 and LINC-ROR in tumors located at distal noncardiac, positive for H. pylori infection, tumors with invasion into the muscle layer of the stomach, and grade II tumor (p < 0.05). CONCLUSION: The clinical results of the SALL4-LINC-ROR association propose a probable functional interaction between these markers in tumor maintenance and aggressiveness. Our study can help to understand one of the mechanisms involved in the progression of GC through the function of these regulators.
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spelling pubmed-81833842021-06-11 Interaction between LINC-ROR and Stemness State in Gastric Cancer Cells with Helicobacter pylori Infection Alsadat Mahmoudian, Reihaneh Lotfi Gharaie, Maryam Abbaszadegan, Roya Forghanifard, Mohammad Mahdi Abbaszadegan, Mohammad Reza Iran Biomed J Full Length BACKGROUND: LINC-ROR, as a cancer-related lncRNA, has vital roles in stem cell survival, pluripotency, differentiation, and self-renewal in hESCs. However, cancer-related molecular mechanisms, its functional roles, and clinical value of LINC-ROR in GC remain unclear. In this study, we aimed to investigate probable interplay between LINC-ROR with SALL4 stemness regulator and their role with the development of the disease. METHODS: The mRNA expression profile of LINC-ROR and SALL4 was assessed in tumoral and adjacent non-cancerous tissues of GC patients, using quantitative real-time PCR. RESULTS: Significant LINC-ROR underexpression and SALL4 overexpression were observed in 55.81% and 75.58% (p < 0.0001) of samples, respectively. The expression of LINC-ROR and SALL4 were significantly correlated with each other (p = 0.044). There was an association between the underexpression of LINC-ROR and sex, stage of tumor progression, tumor type, and location of tumor (p < 0.05), and H. pylori infection with SALL4 expression (p = 0.036). There were also significant correlations between concomitant mRNA expression of SALL4 and LINC-ROR in tumors located at distal noncardiac, positive for H. pylori infection, tumors with invasion into the muscle layer of the stomach, and grade II tumor (p < 0.05). CONCLUSION: The clinical results of the SALL4-LINC-ROR association propose a probable functional interaction between these markers in tumor maintenance and aggressiveness. Our study can help to understand one of the mechanisms involved in the progression of GC through the function of these regulators. Pasteur Institute of Iran 2021-05 2021-03-01 /pmc/articles/PMC8183384/ /pubmed/33745265 http://dx.doi.org/10.52547/ibj.25.3.157 Text en https://creativecommons.org/licenses/by/3.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/ (https://creativecommons.org/licenses/by/3.0/) ) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Full Length
Alsadat Mahmoudian, Reihaneh
Lotfi Gharaie, Maryam
Abbaszadegan, Roya
Forghanifard, Mohammad Mahdi
Abbaszadegan, Mohammad Reza
Interaction between LINC-ROR and Stemness State in Gastric Cancer Cells with Helicobacter pylori Infection
title Interaction between LINC-ROR and Stemness State in Gastric Cancer Cells with Helicobacter pylori Infection
title_full Interaction between LINC-ROR and Stemness State in Gastric Cancer Cells with Helicobacter pylori Infection
title_fullStr Interaction between LINC-ROR and Stemness State in Gastric Cancer Cells with Helicobacter pylori Infection
title_full_unstemmed Interaction between LINC-ROR and Stemness State in Gastric Cancer Cells with Helicobacter pylori Infection
title_short Interaction between LINC-ROR and Stemness State in Gastric Cancer Cells with Helicobacter pylori Infection
title_sort interaction between linc-ror and stemness state in gastric cancer cells with helicobacter pylori infection
topic Full Length
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8183384/
https://www.ncbi.nlm.nih.gov/pubmed/33745265
http://dx.doi.org/10.52547/ibj.25.3.157
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