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The biological role of MutT in the pathogenesis of the zoonotic pathogen Streptococcus suis serotype 2
Streptococcus suis (S. suis) is an important rising pathogen that causes serious diseases in humans and pigs. Although some putative virulence factors of S. suis have been identified, its pathogenic mechanisms are largely unclear. Here, we identified a putative virulence-associated factor MutT, whic...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8183525/ https://www.ncbi.nlm.nih.gov/pubmed/34077309 http://dx.doi.org/10.1080/21505594.2021.1936770 |
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author | Li, Quan Fei, Xia Zhang, Yuhang Guo, Genglin Shi, Huoying Zhang, Wei |
author_facet | Li, Quan Fei, Xia Zhang, Yuhang Guo, Genglin Shi, Huoying Zhang, Wei |
author_sort | Li, Quan |
collection | PubMed |
description | Streptococcus suis (S. suis) is an important rising pathogen that causes serious diseases in humans and pigs. Although some putative virulence factors of S. suis have been identified, its pathogenic mechanisms are largely unclear. Here, we identified a putative virulence-associated factor MutT, which is unique to S. suis serotype 2 (SS2) virulent strains. To investigate the biological roles of MutT in the SS2 virulent strain ZY05719, the mutT knockout mutant (ΔmutT) was generated and used to explore the phenotypic and virulent variations between the parental and ΔmutT strains. We found that the mutT mutation significantly inhibited cell growth ability, shortened the chain length, and displayed a high susceptibility to H(2)O(2)-induced oxidative stress. Moreover, this study revealed that MutT induced the adhesion and invasion of SS2 to host cells. Deletion of mutT increased microbial clearance in host tissues of the infected mice. Sequence alignment results suggested that mutT was encoded in a strain-specific manner, in which the detection was strongly linked to bacterial pathogenicity. In both zebrafish and mice infection models, the virulence of ΔmutT was largely reduced compared with that of ZY05719. Overall, this study provides compelling evidence that MutT is indispensable for the virulence of SS2 and highlights the biological role of MutT in bacteria pathogenesis during infection. |
format | Online Article Text |
id | pubmed-8183525 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-81835252021-06-11 The biological role of MutT in the pathogenesis of the zoonotic pathogen Streptococcus suis serotype 2 Li, Quan Fei, Xia Zhang, Yuhang Guo, Genglin Shi, Huoying Zhang, Wei Virulence Research Paper Streptococcus suis (S. suis) is an important rising pathogen that causes serious diseases in humans and pigs. Although some putative virulence factors of S. suis have been identified, its pathogenic mechanisms are largely unclear. Here, we identified a putative virulence-associated factor MutT, which is unique to S. suis serotype 2 (SS2) virulent strains. To investigate the biological roles of MutT in the SS2 virulent strain ZY05719, the mutT knockout mutant (ΔmutT) was generated and used to explore the phenotypic and virulent variations between the parental and ΔmutT strains. We found that the mutT mutation significantly inhibited cell growth ability, shortened the chain length, and displayed a high susceptibility to H(2)O(2)-induced oxidative stress. Moreover, this study revealed that MutT induced the adhesion and invasion of SS2 to host cells. Deletion of mutT increased microbial clearance in host tissues of the infected mice. Sequence alignment results suggested that mutT was encoded in a strain-specific manner, in which the detection was strongly linked to bacterial pathogenicity. In both zebrafish and mice infection models, the virulence of ΔmutT was largely reduced compared with that of ZY05719. Overall, this study provides compelling evidence that MutT is indispensable for the virulence of SS2 and highlights the biological role of MutT in bacteria pathogenesis during infection. Taylor & Francis 2021-06-02 /pmc/articles/PMC8183525/ /pubmed/34077309 http://dx.doi.org/10.1080/21505594.2021.1936770 Text en © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Paper Li, Quan Fei, Xia Zhang, Yuhang Guo, Genglin Shi, Huoying Zhang, Wei The biological role of MutT in the pathogenesis of the zoonotic pathogen Streptococcus suis serotype 2 |
title | The biological role of MutT in the pathogenesis of the zoonotic pathogen Streptococcus suis serotype 2 |
title_full | The biological role of MutT in the pathogenesis of the zoonotic pathogen Streptococcus suis serotype 2 |
title_fullStr | The biological role of MutT in the pathogenesis of the zoonotic pathogen Streptococcus suis serotype 2 |
title_full_unstemmed | The biological role of MutT in the pathogenesis of the zoonotic pathogen Streptococcus suis serotype 2 |
title_short | The biological role of MutT in the pathogenesis of the zoonotic pathogen Streptococcus suis serotype 2 |
title_sort | biological role of mutt in the pathogenesis of the zoonotic pathogen streptococcus suis serotype 2 |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8183525/ https://www.ncbi.nlm.nih.gov/pubmed/34077309 http://dx.doi.org/10.1080/21505594.2021.1936770 |
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