Cargando…
Clinical performance of ASTA SepsiPrep kit in direct bacterial identification and antimicrobial susceptibility test using MicroIDSys Elite and VITEK‐2 system
BACKGROUND: Rapid and accurate microbial identification and antimicrobial susceptibility testing (AST) are essential for timely use of appropriate antimicrobial agents for bloodstream infection. To shorten the time for isolating colonies from the positive blood culture, various preparation methods f...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8183931/ https://www.ncbi.nlm.nih.gov/pubmed/33939213 http://dx.doi.org/10.1002/jcla.23744 |
_version_ | 1783704485874368512 |
---|---|
author | Yoo, In Young Han, Jayho Ha, Sung Il Cha, Young Jong Pil, Shin Dong Park, Yeon‐Joon |
author_facet | Yoo, In Young Han, Jayho Ha, Sung Il Cha, Young Jong Pil, Shin Dong Park, Yeon‐Joon |
author_sort | Yoo, In Young |
collection | PubMed |
description | BACKGROUND: Rapid and accurate microbial identification and antimicrobial susceptibility testing (AST) are essential for timely use of appropriate antimicrobial agents for bloodstream infection. To shorten the time for isolating colonies from the positive blood culture, various preparation methods for direct identification using matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOF MS) system were developed. Here, we evaluated the SepsiPrep kit (ASTA Corp.) for direct identification of microorganisms and AST from positive blood cultures using MicroIDSys Elite MALDI‐TOF MS system (ASTA Corp.) and VITEK‐2 system (bioMérieux). METHODS: For direct identification, a total of 124 prospective monomicrobial positive blood culture bottles were included. For direct identification, the pellet was prepared by centrifugation and washing twice. For direct AST, the pellet was suspended in 0.45% saline and adjusted to McFarland 0.5. The results from the direct identification and AST using MicroIDSys Elite and VITEK‐2 system were compared to those from the conventional method performed with pure colony subcultured on agar plate. RESULTS: Compared to the conventional method using pure colony, correct direct identification rate was 96.5% and 98.5% for 57 gram‐positive isolates and 67 gram‐negative isolates, respectively. For direct AST, among the 55 gram‐positive isolates, the categorical agreement (CA) for staphylococci, streptococci, and enterococci was 96.7%, 98.4%, and 94.1%, respectively. For 66 gram‐negative isolates, the CA for Enterobacterales and non‐fermentative gram‐negative rods was 99.0% and 96.6%, respectively. CONCLUSIONS: The SepsiPrep kit was easy to use combined with MicroIDSys Elite and VITEK‐2 system and also, the correct identification and AST rate were very high. |
format | Online Article Text |
id | pubmed-8183931 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-81839312021-06-16 Clinical performance of ASTA SepsiPrep kit in direct bacterial identification and antimicrobial susceptibility test using MicroIDSys Elite and VITEK‐2 system Yoo, In Young Han, Jayho Ha, Sung Il Cha, Young Jong Pil, Shin Dong Park, Yeon‐Joon J Clin Lab Anal Brief Report BACKGROUND: Rapid and accurate microbial identification and antimicrobial susceptibility testing (AST) are essential for timely use of appropriate antimicrobial agents for bloodstream infection. To shorten the time for isolating colonies from the positive blood culture, various preparation methods for direct identification using matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOF MS) system were developed. Here, we evaluated the SepsiPrep kit (ASTA Corp.) for direct identification of microorganisms and AST from positive blood cultures using MicroIDSys Elite MALDI‐TOF MS system (ASTA Corp.) and VITEK‐2 system (bioMérieux). METHODS: For direct identification, a total of 124 prospective monomicrobial positive blood culture bottles were included. For direct identification, the pellet was prepared by centrifugation and washing twice. For direct AST, the pellet was suspended in 0.45% saline and adjusted to McFarland 0.5. The results from the direct identification and AST using MicroIDSys Elite and VITEK‐2 system were compared to those from the conventional method performed with pure colony subcultured on agar plate. RESULTS: Compared to the conventional method using pure colony, correct direct identification rate was 96.5% and 98.5% for 57 gram‐positive isolates and 67 gram‐negative isolates, respectively. For direct AST, among the 55 gram‐positive isolates, the categorical agreement (CA) for staphylococci, streptococci, and enterococci was 96.7%, 98.4%, and 94.1%, respectively. For 66 gram‐negative isolates, the CA for Enterobacterales and non‐fermentative gram‐negative rods was 99.0% and 96.6%, respectively. CONCLUSIONS: The SepsiPrep kit was easy to use combined with MicroIDSys Elite and VITEK‐2 system and also, the correct identification and AST rate were very high. John Wiley and Sons Inc. 2021-05-03 /pmc/articles/PMC8183931/ /pubmed/33939213 http://dx.doi.org/10.1002/jcla.23744 Text en © 2021 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Brief Report Yoo, In Young Han, Jayho Ha, Sung Il Cha, Young Jong Pil, Shin Dong Park, Yeon‐Joon Clinical performance of ASTA SepsiPrep kit in direct bacterial identification and antimicrobial susceptibility test using MicroIDSys Elite and VITEK‐2 system |
title | Clinical performance of ASTA SepsiPrep kit in direct bacterial identification and antimicrobial susceptibility test using MicroIDSys Elite and VITEK‐2 system |
title_full | Clinical performance of ASTA SepsiPrep kit in direct bacterial identification and antimicrobial susceptibility test using MicroIDSys Elite and VITEK‐2 system |
title_fullStr | Clinical performance of ASTA SepsiPrep kit in direct bacterial identification and antimicrobial susceptibility test using MicroIDSys Elite and VITEK‐2 system |
title_full_unstemmed | Clinical performance of ASTA SepsiPrep kit in direct bacterial identification and antimicrobial susceptibility test using MicroIDSys Elite and VITEK‐2 system |
title_short | Clinical performance of ASTA SepsiPrep kit in direct bacterial identification and antimicrobial susceptibility test using MicroIDSys Elite and VITEK‐2 system |
title_sort | clinical performance of asta sepsiprep kit in direct bacterial identification and antimicrobial susceptibility test using microidsys elite and vitek‐2 system |
topic | Brief Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8183931/ https://www.ncbi.nlm.nih.gov/pubmed/33939213 http://dx.doi.org/10.1002/jcla.23744 |
work_keys_str_mv | AT yooinyoung clinicalperformanceofastasepsiprepkitindirectbacterialidentificationandantimicrobialsusceptibilitytestusingmicroidsyseliteandvitek2system AT hanjayho clinicalperformanceofastasepsiprepkitindirectbacterialidentificationandantimicrobialsusceptibilitytestusingmicroidsyseliteandvitek2system AT hasungil clinicalperformanceofastasepsiprepkitindirectbacterialidentificationandantimicrobialsusceptibilitytestusingmicroidsyseliteandvitek2system AT chayoungjong clinicalperformanceofastasepsiprepkitindirectbacterialidentificationandantimicrobialsusceptibilitytestusingmicroidsyseliteandvitek2system AT pilshindong clinicalperformanceofastasepsiprepkitindirectbacterialidentificationandantimicrobialsusceptibilitytestusingmicroidsyseliteandvitek2system AT parkyeonjoon clinicalperformanceofastasepsiprepkitindirectbacterialidentificationandantimicrobialsusceptibilitytestusingmicroidsyseliteandvitek2system |