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Profiling of Bile Microbiome Identifies District Microbial Population between Choledocholithiasis and Cholangiocarcinoma Patients

OBJECTIVE: Choledocholithiasis (CDL), a potential risk for cholangiocarcinoma (CCA) development, is often a consequence of bacterial infection. Thus, the microbial population that contributes to CDL might also be involved in CCA development. We compared the microbiome in bile fluid of CDL patients a...

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Autores principales: Dangtakot, Rungtiwa, Intuyod, Kitti, Ahooja, Anucha, Wongwiwatchai, Jitraporn, Hanpanich, Petcharakorn, Lulitanond, Aroonlug, Chamgramol, Yaovalux, Pinlaor, Somchai, Pinlaor, Porntip
Formato: Online Artículo Texto
Lenguaje:English
Publicado: West Asia Organization for Cancer Prevention 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8184169/
https://www.ncbi.nlm.nih.gov/pubmed/33507704
http://dx.doi.org/10.31557/APJCP.2021.22.1.233
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author Dangtakot, Rungtiwa
Intuyod, Kitti
Ahooja, Anucha
Wongwiwatchai, Jitraporn
Hanpanich, Petcharakorn
Lulitanond, Aroonlug
Chamgramol, Yaovalux
Pinlaor, Somchai
Pinlaor, Porntip
author_facet Dangtakot, Rungtiwa
Intuyod, Kitti
Ahooja, Anucha
Wongwiwatchai, Jitraporn
Hanpanich, Petcharakorn
Lulitanond, Aroonlug
Chamgramol, Yaovalux
Pinlaor, Somchai
Pinlaor, Porntip
author_sort Dangtakot, Rungtiwa
collection PubMed
description OBJECTIVE: Choledocholithiasis (CDL), a potential risk for cholangiocarcinoma (CCA) development, is often a consequence of bacterial infection. Thus, the microbial population that contributes to CDL might also be involved in CCA development. We compared the microbiome in bile fluid of CDL patients and CCA patients. METHODS: Bile samples were collected from CDL (n = 30) and CCA (n =30) patients. Microbial profiling was performed individually by the sequencing of V3-V4 regions of the 16S rRNA gene. RESULTS: Enterobacter, Pseudomonas, and Stenotrophomonas species were much more abundant in bile samples from CCA compared to CDL (p<0.05). However, Cetobacterium, Pyramidobacter, and Streptococcus species were less abundant in bile samples of CCA compared to CDL (p<0.05). Although Escherichia was predominant in the CCA, Escherichia coli itself was more abundant in CDL than in CCA. One CDL case (3.3%) harbored genotoxin-producing E. coli as confirmed by PCR. Enterobacter and Pseudomonas also predominated in CCA according to linear discriminant-analysis effect size. CONCLUSION: we demonstrated vast differences between microbial communities in bile of CDL and CCA patients. These bacteria might be partly involved in CCA genesis and may provide novel biomarkers for CCA.
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spelling pubmed-81841692021-06-11 Profiling of Bile Microbiome Identifies District Microbial Population between Choledocholithiasis and Cholangiocarcinoma Patients Dangtakot, Rungtiwa Intuyod, Kitti Ahooja, Anucha Wongwiwatchai, Jitraporn Hanpanich, Petcharakorn Lulitanond, Aroonlug Chamgramol, Yaovalux Pinlaor, Somchai Pinlaor, Porntip Asian Pac J Cancer Prev Research Article OBJECTIVE: Choledocholithiasis (CDL), a potential risk for cholangiocarcinoma (CCA) development, is often a consequence of bacterial infection. Thus, the microbial population that contributes to CDL might also be involved in CCA development. We compared the microbiome in bile fluid of CDL patients and CCA patients. METHODS: Bile samples were collected from CDL (n = 30) and CCA (n =30) patients. Microbial profiling was performed individually by the sequencing of V3-V4 regions of the 16S rRNA gene. RESULTS: Enterobacter, Pseudomonas, and Stenotrophomonas species were much more abundant in bile samples from CCA compared to CDL (p<0.05). However, Cetobacterium, Pyramidobacter, and Streptococcus species were less abundant in bile samples of CCA compared to CDL (p<0.05). Although Escherichia was predominant in the CCA, Escherichia coli itself was more abundant in CDL than in CCA. One CDL case (3.3%) harbored genotoxin-producing E. coli as confirmed by PCR. Enterobacter and Pseudomonas also predominated in CCA according to linear discriminant-analysis effect size. CONCLUSION: we demonstrated vast differences between microbial communities in bile of CDL and CCA patients. These bacteria might be partly involved in CCA genesis and may provide novel biomarkers for CCA. West Asia Organization for Cancer Prevention 2021-01 /pmc/articles/PMC8184169/ /pubmed/33507704 http://dx.doi.org/10.31557/APJCP.2021.22.1.233 Text en https://creativecommons.org/licenses/by/3.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/ (https://creativecommons.org/licenses/by/3.0/) ) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Dangtakot, Rungtiwa
Intuyod, Kitti
Ahooja, Anucha
Wongwiwatchai, Jitraporn
Hanpanich, Petcharakorn
Lulitanond, Aroonlug
Chamgramol, Yaovalux
Pinlaor, Somchai
Pinlaor, Porntip
Profiling of Bile Microbiome Identifies District Microbial Population between Choledocholithiasis and Cholangiocarcinoma Patients
title Profiling of Bile Microbiome Identifies District Microbial Population between Choledocholithiasis and Cholangiocarcinoma Patients
title_full Profiling of Bile Microbiome Identifies District Microbial Population between Choledocholithiasis and Cholangiocarcinoma Patients
title_fullStr Profiling of Bile Microbiome Identifies District Microbial Population between Choledocholithiasis and Cholangiocarcinoma Patients
title_full_unstemmed Profiling of Bile Microbiome Identifies District Microbial Population between Choledocholithiasis and Cholangiocarcinoma Patients
title_short Profiling of Bile Microbiome Identifies District Microbial Population between Choledocholithiasis and Cholangiocarcinoma Patients
title_sort profiling of bile microbiome identifies district microbial population between choledocholithiasis and cholangiocarcinoma patients
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8184169/
https://www.ncbi.nlm.nih.gov/pubmed/33507704
http://dx.doi.org/10.31557/APJCP.2021.22.1.233
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