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Cross-species analysis defines the conservation of anatomically segregated VMH neuron populations
The ventromedial hypothalamic nucleus (VMH) controls diverse behaviors and physiologic functions, suggesting the existence of multiple VMH neural subtypes with distinct functions. Combing translating ribosome affinity purification with RNA-sequencing (TRAP-seq) data with single-nucleus RNA-sequencin...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8184210/ https://www.ncbi.nlm.nih.gov/pubmed/34018926 http://dx.doi.org/10.7554/eLife.69065 |
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author | Affinati, Alison H Sabatini, Paul V True, Cadence Tomlinson, Abigail J Kirigiti, Melissa Lindsley, Sarah R Li, Chien Olson, David P Kievit, Paul Myers, Martin G Rupp, Alan C |
author_facet | Affinati, Alison H Sabatini, Paul V True, Cadence Tomlinson, Abigail J Kirigiti, Melissa Lindsley, Sarah R Li, Chien Olson, David P Kievit, Paul Myers, Martin G Rupp, Alan C |
author_sort | Affinati, Alison H |
collection | PubMed |
description | The ventromedial hypothalamic nucleus (VMH) controls diverse behaviors and physiologic functions, suggesting the existence of multiple VMH neural subtypes with distinct functions. Combing translating ribosome affinity purification with RNA-sequencing (TRAP-seq) data with single-nucleus RNA-sequencing (snRNA-seq) data, we identified 24 mouse VMH neuron clusters. Further analysis, including snRNA-seq data from macaque tissue, defined a more tractable VMH parceling scheme consisting of six major genetically and anatomically differentiated VMH neuron classes with good cross-species conservation. In addition to two major ventrolateral classes, we identified three distinct classes of dorsomedial VMH neurons. Consistent with previously suggested unique roles for leptin receptor (Lepr)-expressing VMH neurons, Lepr expression marked a single dorsomedial class. We also identified a class of glutamatergic VMH neurons that resides in the tuberal region, anterolateral to the neuroanatomical core of the VMH. This atlas of conserved VMH neuron populations provides an unbiased starting point for the analysis of VMH circuitry and function. |
format | Online Article Text |
id | pubmed-8184210 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-81842102021-06-09 Cross-species analysis defines the conservation of anatomically segregated VMH neuron populations Affinati, Alison H Sabatini, Paul V True, Cadence Tomlinson, Abigail J Kirigiti, Melissa Lindsley, Sarah R Li, Chien Olson, David P Kievit, Paul Myers, Martin G Rupp, Alan C eLife Neuroscience The ventromedial hypothalamic nucleus (VMH) controls diverse behaviors and physiologic functions, suggesting the existence of multiple VMH neural subtypes with distinct functions. Combing translating ribosome affinity purification with RNA-sequencing (TRAP-seq) data with single-nucleus RNA-sequencing (snRNA-seq) data, we identified 24 mouse VMH neuron clusters. Further analysis, including snRNA-seq data from macaque tissue, defined a more tractable VMH parceling scheme consisting of six major genetically and anatomically differentiated VMH neuron classes with good cross-species conservation. In addition to two major ventrolateral classes, we identified three distinct classes of dorsomedial VMH neurons. Consistent with previously suggested unique roles for leptin receptor (Lepr)-expressing VMH neurons, Lepr expression marked a single dorsomedial class. We also identified a class of glutamatergic VMH neurons that resides in the tuberal region, anterolateral to the neuroanatomical core of the VMH. This atlas of conserved VMH neuron populations provides an unbiased starting point for the analysis of VMH circuitry and function. eLife Sciences Publications, Ltd 2021-05-21 /pmc/articles/PMC8184210/ /pubmed/34018926 http://dx.doi.org/10.7554/eLife.69065 Text en © 2021, Affinati et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Neuroscience Affinati, Alison H Sabatini, Paul V True, Cadence Tomlinson, Abigail J Kirigiti, Melissa Lindsley, Sarah R Li, Chien Olson, David P Kievit, Paul Myers, Martin G Rupp, Alan C Cross-species analysis defines the conservation of anatomically segregated VMH neuron populations |
title | Cross-species analysis defines the conservation of anatomically segregated VMH neuron populations |
title_full | Cross-species analysis defines the conservation of anatomically segregated VMH neuron populations |
title_fullStr | Cross-species analysis defines the conservation of anatomically segregated VMH neuron populations |
title_full_unstemmed | Cross-species analysis defines the conservation of anatomically segregated VMH neuron populations |
title_short | Cross-species analysis defines the conservation of anatomically segregated VMH neuron populations |
title_sort | cross-species analysis defines the conservation of anatomically segregated vmh neuron populations |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8184210/ https://www.ncbi.nlm.nih.gov/pubmed/34018926 http://dx.doi.org/10.7554/eLife.69065 |
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