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Involvement of Cerebellar Neural Circuits in Active Avoidance Conditioning in Zebrafish
When animals repeatedly receive a combination of neutral conditional stimulus (CS) and aversive unconditional stimulus (US), they learn the relationship between CS and US, and show conditioned fear responses after CS. They show passive responses such as freezing or panic movements (classical or Pavl...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Society for Neuroscience
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8184220/ https://www.ncbi.nlm.nih.gov/pubmed/33952613 http://dx.doi.org/10.1523/ENEURO.0507-20.2021 |
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author | Koyama, Wataru Hosomi, Ryo Matsuda, Koji Kawakami, Koichi Hibi, Masahiko Shimizu, Takashi |
author_facet | Koyama, Wataru Hosomi, Ryo Matsuda, Koji Kawakami, Koichi Hibi, Masahiko Shimizu, Takashi |
author_sort | Koyama, Wataru |
collection | PubMed |
description | When animals repeatedly receive a combination of neutral conditional stimulus (CS) and aversive unconditional stimulus (US), they learn the relationship between CS and US, and show conditioned fear responses after CS. They show passive responses such as freezing or panic movements (classical or Pavlovian fear conditioning), or active behavioral responses to avoid aversive stimuli (active avoidance). Previous studies suggested the roles of the cerebellum in classical fear conditioning but it remains elusive whether the cerebellum is involved in active avoidance conditioning. In this study, we analyzed the roles of cerebellar neural circuits during active avoidance in adult zebrafish. When pairs of CS (light) and US (electric shock) were administered to wild-type zebrafish, about half of them displayed active avoidance. The expression of botulinum toxin, which inhibits the release of neurotransmitters, in cerebellar granule cells (GCs) or Purkinje cells (PCs) did not affect conditioning-independent swimming behaviors, but did inhibit active avoidance conditioning. Nitroreductase (NTR)-mediated ablation of PCs in adult zebrafish also impaired active avoidance. Furthermore, the inhibited transmission of GCs or PCs resulted in reduced fear-conditioned Pavlovian fear responses. Our findings suggest that the zebrafish cerebellum plays an active role in active avoidance conditioning. |
format | Online Article Text |
id | pubmed-8184220 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Society for Neuroscience |
record_format | MEDLINE/PubMed |
spelling | pubmed-81842202021-06-08 Involvement of Cerebellar Neural Circuits in Active Avoidance Conditioning in Zebrafish Koyama, Wataru Hosomi, Ryo Matsuda, Koji Kawakami, Koichi Hibi, Masahiko Shimizu, Takashi eNeuro Research Article: New Research When animals repeatedly receive a combination of neutral conditional stimulus (CS) and aversive unconditional stimulus (US), they learn the relationship between CS and US, and show conditioned fear responses after CS. They show passive responses such as freezing or panic movements (classical or Pavlovian fear conditioning), or active behavioral responses to avoid aversive stimuli (active avoidance). Previous studies suggested the roles of the cerebellum in classical fear conditioning but it remains elusive whether the cerebellum is involved in active avoidance conditioning. In this study, we analyzed the roles of cerebellar neural circuits during active avoidance in adult zebrafish. When pairs of CS (light) and US (electric shock) were administered to wild-type zebrafish, about half of them displayed active avoidance. The expression of botulinum toxin, which inhibits the release of neurotransmitters, in cerebellar granule cells (GCs) or Purkinje cells (PCs) did not affect conditioning-independent swimming behaviors, but did inhibit active avoidance conditioning. Nitroreductase (NTR)-mediated ablation of PCs in adult zebrafish also impaired active avoidance. Furthermore, the inhibited transmission of GCs or PCs resulted in reduced fear-conditioned Pavlovian fear responses. Our findings suggest that the zebrafish cerebellum plays an active role in active avoidance conditioning. Society for Neuroscience 2021-06-02 /pmc/articles/PMC8184220/ /pubmed/33952613 http://dx.doi.org/10.1523/ENEURO.0507-20.2021 Text en Copyright © 2021 Koyama et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
spellingShingle | Research Article: New Research Koyama, Wataru Hosomi, Ryo Matsuda, Koji Kawakami, Koichi Hibi, Masahiko Shimizu, Takashi Involvement of Cerebellar Neural Circuits in Active Avoidance Conditioning in Zebrafish |
title | Involvement of Cerebellar Neural Circuits in Active Avoidance Conditioning in Zebrafish |
title_full | Involvement of Cerebellar Neural Circuits in Active Avoidance Conditioning in Zebrafish |
title_fullStr | Involvement of Cerebellar Neural Circuits in Active Avoidance Conditioning in Zebrafish |
title_full_unstemmed | Involvement of Cerebellar Neural Circuits in Active Avoidance Conditioning in Zebrafish |
title_short | Involvement of Cerebellar Neural Circuits in Active Avoidance Conditioning in Zebrafish |
title_sort | involvement of cerebellar neural circuits in active avoidance conditioning in zebrafish |
topic | Research Article: New Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8184220/ https://www.ncbi.nlm.nih.gov/pubmed/33952613 http://dx.doi.org/10.1523/ENEURO.0507-20.2021 |
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