Cargando…

Capsid integrity RT-qPCR for the selective detection of intact SARS-CoV-2 in wastewater

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) genomes have been detected in wastewater worldwide. However, the assessment of SARS-CoV-2 infectivity in wastewater has been limited due to the stringent requirements of biosafety level 3. The main objective of this study is to investigate...

Descripción completa

Detalles Bibliográficos
Autores principales:	Canh, Vu Duc, Torii, Shotaro, Yasui, Midori, Kyuwa, Shigeru, Katayama, Hiroyuki
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	The Authors. Published by Elsevier B.V. 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8184355/ https://www.ncbi.nlm.nih.gov/pubmed/34139497 http://dx.doi.org/10.1016/j.scitotenv.2021.148342

Ejemplares similares

Viability RT-qPCR to Distinguish Between HEV and HAV With Intact and Altered Capsids
por: Randazzo, Walter, et al.
Publicado: (2018)

Comparison of five polyethylene glycol precipitation procedures for the RT-qPCR based recovery of murine hepatitis virus, bacteriophage phi6, and pepper mild mottle virus as a surrogate for SARS-CoV-2 from wastewater
por: Torii, Shotaro, et al.
Publicado: (2022)

Detection and identification of potentially infectious gastrointestinal and respiratory viruses at workplaces of wastewater treatment plants with viability qPCR/RT-qPCR
por: Stobnicka-Kupiec, Agata, et al.
Publicado: (2022)

Assessing sensitivity and reproducibility of RT-ddPCR and RT-qPCR for the quantification of SARS-CoV-2 in wastewater
por: Ciesielski, Mark, et al.
Publicado: (2021)

Comparison of RT-qPCR and RT-dPCR Platforms for the Trace Detection of SARS-CoV-2 RNA in Wastewater
por: Ahmed, Warish, et al.
Publicado: (2022)

Tutorial: Guidelines for Single-Cell RT-qPCR
por: Zucha, Daniel, et al.
Publicado: (2021)

Evaluation of process limit of detection and quantification variation of SARS-CoV-2 RT-qPCR and RT-dPCR assays for wastewater surveillance
por: Ahmed, Warish, et al.
Publicado: (2022)

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR
por: Fout, G. Shay, et al.
Publicado: (2016)

RT-qPCR and ATOPlex sequencing for the sensitive detection of SARS-CoV-2 RNA for wastewater surveillance
por: Ahmed, Warish, et al.
Publicado: (2022)

Algorithm Optimization in Methylation Detection with Multiple RT-qPCR
por: Song, Lele, et al.
Publicado: (2016)

Improving the standardization of mRNA measurement by RT-qPCR
por: Sanders, Rebecca, et al.
Publicado: (2018)

COVID-19 diagnosis by RT-qPCR in alternative specimens
por: Gonçalves, Cássia Cristina Alves, et al.
Publicado: (2021)

Rapid and accurate quantification of isomiRs by RT-qPCR
por: Franco, Sandra, et al.
Publicado: (2022)

Comparison of RT-dPCR and RT-qPCR and the effects of freeze–thaw cycle and glycine release buffer for wastewater SARS-CoV-2 analysis
por: Huge, Bonnie Jaskowski, et al.
Publicado: (2022)

Evaluation of the mechanism of action of Bacillus spp. to manage Meloidogyne incognita with split root assay, RT-qPCR and qPCR
por: Gattoni, Kaitlin M., et al.
Publicado: (2023)

SASqPCR: Robust and Rapid Analysis of RT-qPCR Data in SAS
por: Ling, Daijun
Publicado: (2012)

Comparison of RT-qPCR and RT-ddPCR with Rift valley fever virus (RVFV) RNA
por: Park, Changwoo, et al.
Publicado: (2023)

Clinical Evaluation of Self-Collected Saliva by Quantitative Reverse Transcription-PCR (RT-qPCR), Direct RT-qPCR, Reverse Transcription–Loop-Mediated Isothermal Amplification, and a Rapid Antigen Test To Diagnose COVID-19
por: Nagura-Ikeda, Mayu, et al.
Publicado: (2020)

Mathematical modeling based on RT-qPCR analysis of SARS-CoV-2 in wastewater as a tool for epidemiology
por: Krivoňáková, Naďa, et al.
Publicado: (2021)

Selection of Suitable Reference Genes for RT-qPCR Analyses in Cyanobacteria
por: Pinto, Filipe, et al.
Publicado: (2012)

Applicability of RNA standards for evaluating RT-qPCR assays and platforms
por: Devonshire, Alison S, et al.
Publicado: (2011)

RT-qPCR Normalization Genes in the Red Alga Chondrus crispus
por: Kowalczyk, Nathalie, et al.
Publicado: (2014)

Hepatitis A virus subgenotyping based on RT-qPCR assays
por: Coudray-Meunier, Coralie, et al.
Publicado: (2014)

Custom RT-qPCR-array for glaucoma filtering surgery prognosis
por: Rodriguez-Agirretxe, Iñaki, et al.
Publicado: (2017)

Linear-hairpin variable primer RT-qPCR for MicroRNA
por: Lan, Lin, et al.
Publicado: (2018)

Predictive factors of COVID-19 in patients with negative RT-qPCR
por: López de la Iglesia, J., et al.
Publicado: (2020)

Large-Scale RT-qPCR Diagnostics for Seed Potato Certification
por: Schumpp, Olivier, et al.
Publicado: (2021)

Sampling, Logistics, and Analytics of Urine for RT-qPCR-based Diagnostics
por: Kretschmer-Kazemi Far, Rosel, et al.
Publicado: (2021)

RT-qPCR-based pool testing for the diagnosis of COVID-19
por: Sato, Hugo Itaru, et al.
Publicado: (2023)

Detection of Cytoplasmic and Nuclear Circular RNA via RT-qPCR
por: Tan, Ke-En, et al.
Publicado: (2023)

Development of RT-qPCR and semi-nested RT-PCR assays for molecular diagnosis of hantavirus pulmonary syndrome
por: Nunes, Bruno Tardelli Diniz, et al.
Publicado: (2019)

NormQ: RNASeq normalization based on RT-qPCR derived size factors
por: Naraine, Ravindra, et al.
Publicado: (2020)

Analytical performance comparison of four SARS-CoV-2 RT-qPCR primer-probe sets for wastewater samples
por: Zhang, Shuxin, et al.
Publicado: (2022)

RT-qPCR assays for SARS-CoV-2 variants of concern in wastewater reveals compromised vaccination-induced immunity
por: Yaniv, Karin, et al.
Publicado: (2021)

Variability in RT-qPCR assay parameters indicates unreliable SARS-CoV-2 RNA quantification for wastewater surveillance
por: Bivins, Aaron, et al.
Publicado: (2021)

Discrimination and surveillance of infectious severe acute respiratory syndrome Coronavirus 2 in wastewater using cell culture and RT-qPCR
por: Monteiro, Silvia, et al.
Publicado: (2022)

The Inhibition and Variability of Two Different RT-qPCR Assays Used for Quantifying SARS-CoV-2 RNA in Wastewater
por: Scott, George, et al.
Publicado: (2023)

Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies
por: Paiva, Marcelo Henrique Santos, et al.
Publicado: (2017)

Identification of Endogenous Reference Genes for RT-qPCR Expression Analysis in Urochloa brizantha Under Abiotic Stresses
por: Takamori, Luciana Midori, et al.
Publicado: (2017)

Mobile Efficient Diagnostics of Infectious Diseases via On‐Chip RT‐qPCR: MEDIC‐PCR
por: Shrestha, Kiran, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_967sq1r2v1ppn291j47lc13ajd