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The therapeutic effects and mechanisms of Long Chai Fang on chronic hepatitis B

BACKGROUND: Long Chai Fang (LCF) is a traditional Chinese medicine (TCM) formula for treating chronic hepatitis B (CHB) in clinical settings; however, its related mechanism remains unclear. METHODS: To address this issue, network pharmacology and an integrative method that combines dot-blot hybridiz...

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Autores principales: Xu, Tingting, Wang, Pei, Zheng, Xue, Yan, Zhanpeng, Li, Kun, Xu, Jindi, Jiang, Cuihua, Zhu, Fangshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AME Publishing Company 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8184409/
https://www.ncbi.nlm.nih.gov/pubmed/34164499
http://dx.doi.org/10.21037/atm-21-1923
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author Xu, Tingting
Wang, Pei
Zheng, Xue
Yan, Zhanpeng
Li, Kun
Xu, Jindi
Jiang, Cuihua
Zhu, Fangshi
author_facet Xu, Tingting
Wang, Pei
Zheng, Xue
Yan, Zhanpeng
Li, Kun
Xu, Jindi
Jiang, Cuihua
Zhu, Fangshi
author_sort Xu, Tingting
collection PubMed
description BACKGROUND: Long Chai Fang (LCF) is a traditional Chinese medicine (TCM) formula for treating chronic hepatitis B (CHB) in clinical settings; however, its related mechanism remains unclear. METHODS: To address this issue, network pharmacology and an integrative method that combines dot-blot hybridization and metabolomics analysis were employed. Network pharmacology was performed to investigate the material basis and potential mechanisms of LCF against CHB. The effect of LCF on Duck hepatitis B virus (DHBV) replication was evaluated. The metabolomics analysis was conducted to identify potential biomarkers in duck serum. RESULTS: The network pharmacology approach revealed 133 potential active components, 897 drug targets, 979 disease targets, and 185 drug-disease targets, while the Kyoto Encyclopedia of Genes and Genomes enrichment analysis identified 165 pathways. LCF significantly inhibited DHBV-deoxyribonucleic acid replication on day 10 and day 3 after the cessation of treatment. Notably, the low-dose LCF group showed the best inhibitory effect. The obviously sustained anti-DHBV activity of LCF inhibited viral replication, and a rebound reaction was found. Phosphatidylcholine and phosphatidylethanolamine classes, which are mainly involved in liver cell repair and energy metabolism through phospholipid metabolic pathways, were identified by metabolomics analysis. CONCLUSIONS: our results showed that the main active ingredients of LCF appear to be metacarpi, isorhamnetin, glypallichalcone, and phaseolinisoflavan. This study provides novel strategies for using a LCF formula against CHB in future research.
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spelling pubmed-81844092021-06-22 The therapeutic effects and mechanisms of Long Chai Fang on chronic hepatitis B Xu, Tingting Wang, Pei Zheng, Xue Yan, Zhanpeng Li, Kun Xu, Jindi Jiang, Cuihua Zhu, Fangshi Ann Transl Med Original Article BACKGROUND: Long Chai Fang (LCF) is a traditional Chinese medicine (TCM) formula for treating chronic hepatitis B (CHB) in clinical settings; however, its related mechanism remains unclear. METHODS: To address this issue, network pharmacology and an integrative method that combines dot-blot hybridization and metabolomics analysis were employed. Network pharmacology was performed to investigate the material basis and potential mechanisms of LCF against CHB. The effect of LCF on Duck hepatitis B virus (DHBV) replication was evaluated. The metabolomics analysis was conducted to identify potential biomarkers in duck serum. RESULTS: The network pharmacology approach revealed 133 potential active components, 897 drug targets, 979 disease targets, and 185 drug-disease targets, while the Kyoto Encyclopedia of Genes and Genomes enrichment analysis identified 165 pathways. LCF significantly inhibited DHBV-deoxyribonucleic acid replication on day 10 and day 3 after the cessation of treatment. Notably, the low-dose LCF group showed the best inhibitory effect. The obviously sustained anti-DHBV activity of LCF inhibited viral replication, and a rebound reaction was found. Phosphatidylcholine and phosphatidylethanolamine classes, which are mainly involved in liver cell repair and energy metabolism through phospholipid metabolic pathways, were identified by metabolomics analysis. CONCLUSIONS: our results showed that the main active ingredients of LCF appear to be metacarpi, isorhamnetin, glypallichalcone, and phaseolinisoflavan. This study provides novel strategies for using a LCF formula against CHB in future research. AME Publishing Company 2021-05 /pmc/articles/PMC8184409/ /pubmed/34164499 http://dx.doi.org/10.21037/atm-21-1923 Text en 2021 Annals of Translational Medicine. All rights reserved. https://creativecommons.org/licenses/by-nc-nd/4.0/Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Original Article
Xu, Tingting
Wang, Pei
Zheng, Xue
Yan, Zhanpeng
Li, Kun
Xu, Jindi
Jiang, Cuihua
Zhu, Fangshi
The therapeutic effects and mechanisms of Long Chai Fang on chronic hepatitis B
title The therapeutic effects and mechanisms of Long Chai Fang on chronic hepatitis B
title_full The therapeutic effects and mechanisms of Long Chai Fang on chronic hepatitis B
title_fullStr The therapeutic effects and mechanisms of Long Chai Fang on chronic hepatitis B
title_full_unstemmed The therapeutic effects and mechanisms of Long Chai Fang on chronic hepatitis B
title_short The therapeutic effects and mechanisms of Long Chai Fang on chronic hepatitis B
title_sort therapeutic effects and mechanisms of long chai fang on chronic hepatitis b
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8184409/
https://www.ncbi.nlm.nih.gov/pubmed/34164499
http://dx.doi.org/10.21037/atm-21-1923
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