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Identifying molecular pathways and candidate genes associated with knob traits by transcriptome analysis in the goose (Anser cygnoides)

Anser cygnoides has a spherical crest on the beak roof, which is described as knob. However, the mechanisms affecting knob morphology are unclear. Here, we investigated the phenotypic characteristics and molecular basis of knob-size differences in Yangzhou geese. Anatomically, the knob was identifie...

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Autores principales: Ji, Wangyang, Hou, li E, Yuan, Xiaoya, Gu, Tiantian, Chen, ZhuoYu, Zhang, Yu, Zhang, Yang, Chen, Guohong, Xu, Qi, Zhao, Wenming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8184827/
https://www.ncbi.nlm.nih.gov/pubmed/34099774
http://dx.doi.org/10.1038/s41598-021-91269-1
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author Ji, Wangyang
Hou, li E
Yuan, Xiaoya
Gu, Tiantian
Chen, ZhuoYu
Zhang, Yu
Zhang, Yang
Chen, Guohong
Xu, Qi
Zhao, Wenming
author_facet Ji, Wangyang
Hou, li E
Yuan, Xiaoya
Gu, Tiantian
Chen, ZhuoYu
Zhang, Yu
Zhang, Yang
Chen, Guohong
Xu, Qi
Zhao, Wenming
author_sort Ji, Wangyang
collection PubMed
description Anser cygnoides has a spherical crest on the beak roof, which is described as knob. However, the mechanisms affecting knob morphology are unclear. Here, we investigated the phenotypic characteristics and molecular basis of knob-size differences in Yangzhou geese. Anatomically, the knob was identified as frontal hump in the frontal area of the skull, rather than hump of upper beak. Although the frontal hump length, and height varied greatly in geese with different knob phenotypes, little was changed in the width. Histologically, knob skin in large-size knobs geese have a greater length in the stratum corneum, stratum spinosum, and stratum reticular than that in small-size knobs geese. Moveover, the 415 differentially expressed genes were found between the large knobs and small ones through transcriptome profiling. In addition, GO enrichment and KEGG pathway analysis revealed 455 significant GO terms and 210 KEGG pathways were enriched, respectively. Among these, TGF-β signaling and thyroid hormone synthesis-signaling pathways were identified to determine knob-size phenotype. Furthermore, BMP5, DCN, TSHR and ADCY3 were recognized to involve in the growth and development of knob. Our data provide comprehensive molecular determinants of knob size phenotype, which can potentially promote the genetic improvement of goose knobs.
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spelling pubmed-81848272021-06-08 Identifying molecular pathways and candidate genes associated with knob traits by transcriptome analysis in the goose (Anser cygnoides) Ji, Wangyang Hou, li E Yuan, Xiaoya Gu, Tiantian Chen, ZhuoYu Zhang, Yu Zhang, Yang Chen, Guohong Xu, Qi Zhao, Wenming Sci Rep Article Anser cygnoides has a spherical crest on the beak roof, which is described as knob. However, the mechanisms affecting knob morphology are unclear. Here, we investigated the phenotypic characteristics and molecular basis of knob-size differences in Yangzhou geese. Anatomically, the knob was identified as frontal hump in the frontal area of the skull, rather than hump of upper beak. Although the frontal hump length, and height varied greatly in geese with different knob phenotypes, little was changed in the width. Histologically, knob skin in large-size knobs geese have a greater length in the stratum corneum, stratum spinosum, and stratum reticular than that in small-size knobs geese. Moveover, the 415 differentially expressed genes were found between the large knobs and small ones through transcriptome profiling. In addition, GO enrichment and KEGG pathway analysis revealed 455 significant GO terms and 210 KEGG pathways were enriched, respectively. Among these, TGF-β signaling and thyroid hormone synthesis-signaling pathways were identified to determine knob-size phenotype. Furthermore, BMP5, DCN, TSHR and ADCY3 were recognized to involve in the growth and development of knob. Our data provide comprehensive molecular determinants of knob size phenotype, which can potentially promote the genetic improvement of goose knobs. Nature Publishing Group UK 2021-06-07 /pmc/articles/PMC8184827/ /pubmed/34099774 http://dx.doi.org/10.1038/s41598-021-91269-1 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Ji, Wangyang
Hou, li E
Yuan, Xiaoya
Gu, Tiantian
Chen, ZhuoYu
Zhang, Yu
Zhang, Yang
Chen, Guohong
Xu, Qi
Zhao, Wenming
Identifying molecular pathways and candidate genes associated with knob traits by transcriptome analysis in the goose (Anser cygnoides)
title Identifying molecular pathways and candidate genes associated with knob traits by transcriptome analysis in the goose (Anser cygnoides)
title_full Identifying molecular pathways and candidate genes associated with knob traits by transcriptome analysis in the goose (Anser cygnoides)
title_fullStr Identifying molecular pathways and candidate genes associated with knob traits by transcriptome analysis in the goose (Anser cygnoides)
title_full_unstemmed Identifying molecular pathways and candidate genes associated with knob traits by transcriptome analysis in the goose (Anser cygnoides)
title_short Identifying molecular pathways and candidate genes associated with knob traits by transcriptome analysis in the goose (Anser cygnoides)
title_sort identifying molecular pathways and candidate genes associated with knob traits by transcriptome analysis in the goose (anser cygnoides)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8184827/
https://www.ncbi.nlm.nih.gov/pubmed/34099774
http://dx.doi.org/10.1038/s41598-021-91269-1
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