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Biomimetic Nanopillar-Based Biosensor for Label-Free Detection of Influenza A Virus
Since the first emergence of influenza viruses, they have caused the flu seasonally worldwide. Precise detection of influenza viruses is required to prevent the spreading of the disease. Herein, we developed an optical biosensor using peptide-immobilized nanopillar structures for the label-free dete...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean BioChip Society (KBCS)
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8184868/ https://www.ncbi.nlm.nih.gov/pubmed/34122741 http://dx.doi.org/10.1007/s13206-021-00027-y |
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author | Lee, Wang Sik Ahn, Junhyoung Jung, Sanghee Lee, Jaejong Kang, Taejoon Jeong, Jinyoung |
author_facet | Lee, Wang Sik Ahn, Junhyoung Jung, Sanghee Lee, Jaejong Kang, Taejoon Jeong, Jinyoung |
author_sort | Lee, Wang Sik |
collection | PubMed |
description | Since the first emergence of influenza viruses, they have caused the flu seasonally worldwide. Precise detection of influenza viruses is required to prevent the spreading of the disease. Herein, we developed an optical biosensor using peptide-immobilized nanopillar structures for the label-free detection of influenza viruses. The spin-on-glass nanopillar structures were fabricated by nanoimprint lithography. A sialic acid-mimic peptide, which can specifically bind to hemagglutinin on the surface of the influenza virus, was immobilized onto the nanopillars via polymerized dopamine. The constructed nanopillar sensor enabled us to detect influenza A viruses in the range of 10(3)–10(5) plaque-forming units through simple measurements of reflectance. Our findings suggest that biomimetic modification of nanopillar structures can be an alternative method for the immunodiagnosis of influenza viruses. |
format | Online Article Text |
id | pubmed-8184868 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | The Korean BioChip Society (KBCS) |
record_format | MEDLINE/PubMed |
spelling | pubmed-81848682021-06-08 Biomimetic Nanopillar-Based Biosensor for Label-Free Detection of Influenza A Virus Lee, Wang Sik Ahn, Junhyoung Jung, Sanghee Lee, Jaejong Kang, Taejoon Jeong, Jinyoung Biochip J Original Article Since the first emergence of influenza viruses, they have caused the flu seasonally worldwide. Precise detection of influenza viruses is required to prevent the spreading of the disease. Herein, we developed an optical biosensor using peptide-immobilized nanopillar structures for the label-free detection of influenza viruses. The spin-on-glass nanopillar structures were fabricated by nanoimprint lithography. A sialic acid-mimic peptide, which can specifically bind to hemagglutinin on the surface of the influenza virus, was immobilized onto the nanopillars via polymerized dopamine. The constructed nanopillar sensor enabled us to detect influenza A viruses in the range of 10(3)–10(5) plaque-forming units through simple measurements of reflectance. Our findings suggest that biomimetic modification of nanopillar structures can be an alternative method for the immunodiagnosis of influenza viruses. The Korean BioChip Society (KBCS) 2021-06-08 2021 /pmc/articles/PMC8184868/ /pubmed/34122741 http://dx.doi.org/10.1007/s13206-021-00027-y Text en © The Author(s), under exclusive licence to The Korean BioChip Society 2021 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Original Article Lee, Wang Sik Ahn, Junhyoung Jung, Sanghee Lee, Jaejong Kang, Taejoon Jeong, Jinyoung Biomimetic Nanopillar-Based Biosensor for Label-Free Detection of Influenza A Virus |
title | Biomimetic Nanopillar-Based Biosensor for Label-Free Detection of Influenza A Virus |
title_full | Biomimetic Nanopillar-Based Biosensor for Label-Free Detection of Influenza A Virus |
title_fullStr | Biomimetic Nanopillar-Based Biosensor for Label-Free Detection of Influenza A Virus |
title_full_unstemmed | Biomimetic Nanopillar-Based Biosensor for Label-Free Detection of Influenza A Virus |
title_short | Biomimetic Nanopillar-Based Biosensor for Label-Free Detection of Influenza A Virus |
title_sort | biomimetic nanopillar-based biosensor for label-free detection of influenza a virus |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8184868/ https://www.ncbi.nlm.nih.gov/pubmed/34122741 http://dx.doi.org/10.1007/s13206-021-00027-y |
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