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Multiplexed live-cell profiling with Raman probes

Single-cell multiparameter measurement has been increasingly recognized as a key technology toward systematic understandings of complex molecular and cellular functions in biological systems. Despite extensive efforts in analytical techniques, it is still generally challenging for existing methods t...

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Autores principales: Chen, Chen, Zhao, Zhilun, Qian, Naixin, Wei, Shixuan, Hu, Fanghao, Min, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8184955/
https://www.ncbi.nlm.nih.gov/pubmed/34099708
http://dx.doi.org/10.1038/s41467-021-23700-0
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author Chen, Chen
Zhao, Zhilun
Qian, Naixin
Wei, Shixuan
Hu, Fanghao
Min, Wei
author_facet Chen, Chen
Zhao, Zhilun
Qian, Naixin
Wei, Shixuan
Hu, Fanghao
Min, Wei
author_sort Chen, Chen
collection PubMed
description Single-cell multiparameter measurement has been increasingly recognized as a key technology toward systematic understandings of complex molecular and cellular functions in biological systems. Despite extensive efforts in analytical techniques, it is still generally challenging for existing methods to decipher a large number of phenotypes in a single living cell. Herein we devise a multiplexed Raman probe panel with sharp and mutually resolvable Raman peaks to simultaneously quantify cell surface proteins, endocytosis activities, and metabolic dynamics of an individual live cell. When coupling it to whole-cell spontaneous Raman micro-spectroscopy, we demonstrate the utility of this technique in 14-plexed live-cell profiling and phenotyping under various drug perturbations. In particular, single-cell multiparameter measurement enables powerful clustering, correlation, and network analysis with biological insights. This profiling platform is compatible with live-cell cytometry, of low instrument complexity and capable of highly multiplexed measurement in a robust and straightforward manner, thereby contributing a valuable tool for both basic single-cell biology and translation applications such as high-content cell sorting and drug discovery.
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spelling pubmed-81849552021-06-11 Multiplexed live-cell profiling with Raman probes Chen, Chen Zhao, Zhilun Qian, Naixin Wei, Shixuan Hu, Fanghao Min, Wei Nat Commun Article Single-cell multiparameter measurement has been increasingly recognized as a key technology toward systematic understandings of complex molecular and cellular functions in biological systems. Despite extensive efforts in analytical techniques, it is still generally challenging for existing methods to decipher a large number of phenotypes in a single living cell. Herein we devise a multiplexed Raman probe panel with sharp and mutually resolvable Raman peaks to simultaneously quantify cell surface proteins, endocytosis activities, and metabolic dynamics of an individual live cell. When coupling it to whole-cell spontaneous Raman micro-spectroscopy, we demonstrate the utility of this technique in 14-plexed live-cell profiling and phenotyping under various drug perturbations. In particular, single-cell multiparameter measurement enables powerful clustering, correlation, and network analysis with biological insights. This profiling platform is compatible with live-cell cytometry, of low instrument complexity and capable of highly multiplexed measurement in a robust and straightforward manner, thereby contributing a valuable tool for both basic single-cell biology and translation applications such as high-content cell sorting and drug discovery. Nature Publishing Group UK 2021-06-07 /pmc/articles/PMC8184955/ /pubmed/34099708 http://dx.doi.org/10.1038/s41467-021-23700-0 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Chen, Chen
Zhao, Zhilun
Qian, Naixin
Wei, Shixuan
Hu, Fanghao
Min, Wei
Multiplexed live-cell profiling with Raman probes
title Multiplexed live-cell profiling with Raman probes
title_full Multiplexed live-cell profiling with Raman probes
title_fullStr Multiplexed live-cell profiling with Raman probes
title_full_unstemmed Multiplexed live-cell profiling with Raman probes
title_short Multiplexed live-cell profiling with Raman probes
title_sort multiplexed live-cell profiling with raman probes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8184955/
https://www.ncbi.nlm.nih.gov/pubmed/34099708
http://dx.doi.org/10.1038/s41467-021-23700-0
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