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Electricity-producing Staphylococcus epidermidis counteracts Cutibacterium acnes

Staphylococcus epidermidis (S. epidermidis) ATCC 12228 was incubated with 2% polyethylene glycol (PEG)-8 Laurate to yield electricity which was measured by a voltage difference between electrodes. Production of electron was validated by a Ferrozine assay. The anti-Cutibacterium acnes (C. acnes) acti...

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Autores principales: Marito, Shinta, Keshari, Sunita, Traisaeng, Supitchaya, My, Do Thi Tra, Balasubramaniam, Arun, Adi, Prakoso, Hsieh, Ming-Fa, Herr, Deron Raymond, Huang, Chun-Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8184966/
https://www.ncbi.nlm.nih.gov/pubmed/34099817
http://dx.doi.org/10.1038/s41598-021-91398-7
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author Marito, Shinta
Keshari, Sunita
Traisaeng, Supitchaya
My, Do Thi Tra
Balasubramaniam, Arun
Adi, Prakoso
Hsieh, Ming-Fa
Herr, Deron Raymond
Huang, Chun-Ming
author_facet Marito, Shinta
Keshari, Sunita
Traisaeng, Supitchaya
My, Do Thi Tra
Balasubramaniam, Arun
Adi, Prakoso
Hsieh, Ming-Fa
Herr, Deron Raymond
Huang, Chun-Ming
author_sort Marito, Shinta
collection PubMed
description Staphylococcus epidermidis (S. epidermidis) ATCC 12228 was incubated with 2% polyethylene glycol (PEG)-8 Laurate to yield electricity which was measured by a voltage difference between electrodes. Production of electron was validated by a Ferrozine assay. The anti-Cutibacterium acnes (C. acnes) activity of electrogenic S. epidermidis was assessed in vitro and in vivo. The voltage change (~ 4.4 mV) reached a peak 60 min after pipetting S. epidermidis plus 2% PEG-8 Laurate onto anodes. The electricity produced by S. epidermidis caused significant growth attenuation and cell lysis of C. acnes. Intradermal injection of C. acnes and S. epidermidis plus PEG-8 Laurate into the mouse ear considerably suppressed the growth of C. acnes. This suppressive effect was noticeably reversed when cyclophilin A of S. epidermidis was inhibited, indicating the essential role of cyclophilin A in electricity production of S. epidermidis against C. acnes. In summary, we demonstrate for the first time that skin S. epidermidis, in the presence of PEG-8 Laurate, can mediate cyclophilin A to elicit an electrical current that has anti-C. acnes effects. Electricity generated by S. epidermidis may confer immediate innate immunity in acne lesions to rein in the overgrowth of C. acnes at the onset of acne vulgaris.
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spelling pubmed-81849662021-06-08 Electricity-producing Staphylococcus epidermidis counteracts Cutibacterium acnes Marito, Shinta Keshari, Sunita Traisaeng, Supitchaya My, Do Thi Tra Balasubramaniam, Arun Adi, Prakoso Hsieh, Ming-Fa Herr, Deron Raymond Huang, Chun-Ming Sci Rep Article Staphylococcus epidermidis (S. epidermidis) ATCC 12228 was incubated with 2% polyethylene glycol (PEG)-8 Laurate to yield electricity which was measured by a voltage difference between electrodes. Production of electron was validated by a Ferrozine assay. The anti-Cutibacterium acnes (C. acnes) activity of electrogenic S. epidermidis was assessed in vitro and in vivo. The voltage change (~ 4.4 mV) reached a peak 60 min after pipetting S. epidermidis plus 2% PEG-8 Laurate onto anodes. The electricity produced by S. epidermidis caused significant growth attenuation and cell lysis of C. acnes. Intradermal injection of C. acnes and S. epidermidis plus PEG-8 Laurate into the mouse ear considerably suppressed the growth of C. acnes. This suppressive effect was noticeably reversed when cyclophilin A of S. epidermidis was inhibited, indicating the essential role of cyclophilin A in electricity production of S. epidermidis against C. acnes. In summary, we demonstrate for the first time that skin S. epidermidis, in the presence of PEG-8 Laurate, can mediate cyclophilin A to elicit an electrical current that has anti-C. acnes effects. Electricity generated by S. epidermidis may confer immediate innate immunity in acne lesions to rein in the overgrowth of C. acnes at the onset of acne vulgaris. Nature Publishing Group UK 2021-06-07 /pmc/articles/PMC8184966/ /pubmed/34099817 http://dx.doi.org/10.1038/s41598-021-91398-7 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Marito, Shinta
Keshari, Sunita
Traisaeng, Supitchaya
My, Do Thi Tra
Balasubramaniam, Arun
Adi, Prakoso
Hsieh, Ming-Fa
Herr, Deron Raymond
Huang, Chun-Ming
Electricity-producing Staphylococcus epidermidis counteracts Cutibacterium acnes
title Electricity-producing Staphylococcus epidermidis counteracts Cutibacterium acnes
title_full Electricity-producing Staphylococcus epidermidis counteracts Cutibacterium acnes
title_fullStr Electricity-producing Staphylococcus epidermidis counteracts Cutibacterium acnes
title_full_unstemmed Electricity-producing Staphylococcus epidermidis counteracts Cutibacterium acnes
title_short Electricity-producing Staphylococcus epidermidis counteracts Cutibacterium acnes
title_sort electricity-producing staphylococcus epidermidis counteracts cutibacterium acnes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8184966/
https://www.ncbi.nlm.nih.gov/pubmed/34099817
http://dx.doi.org/10.1038/s41598-021-91398-7
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