Cargando…
Lipopolysaccharide stimulation test on cultured PBMCs assists the discrimination of cryopyrin-associated periodic syndrome from systemic juvenile idiopathic arthritis
Systemic juvenile idiopathic arthritis (sJIA) and cryopyrin-associated periodic syndrome (CAPS) share many common manifestations. We aim to identify an applicable method to assist disease discrimination. Inflammatory cytokines were measured in the plasma of patients with CAPS, sJIA with persistent d...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185076/ https://www.ncbi.nlm.nih.gov/pubmed/34099791 http://dx.doi.org/10.1038/s41598-021-91354-5 |
_version_ | 1783704710566379520 |
---|---|
author | Wu, Chao-Yi Fan, Wen-Lang Chiu, Ying-Ming Yang, Huang-Yu Lee, Wen-I. Huang, Jing-Long |
author_facet | Wu, Chao-Yi Fan, Wen-Lang Chiu, Ying-Ming Yang, Huang-Yu Lee, Wen-I. Huang, Jing-Long |
author_sort | Wu, Chao-Yi |
collection | PubMed |
description | Systemic juvenile idiopathic arthritis (sJIA) and cryopyrin-associated periodic syndrome (CAPS) share many common manifestations. We aim to identify an applicable method to assist disease discrimination. Inflammatory cytokines were measured in the plasma of patients with CAPS, sJIA with persistent disease course and healthy controls. Supernatants collected from non-stimulated peripheral blood mononuclear cells (PBMCs) and those undergone inflammasome stimulation tests utilizing lipopolysaccharide (LPS) with and without adenosine triphosphate (ATP) were investigated. Inflammatory cytokines in patient plasma fail to differentiate sJIA from CAPS. PBMCs from sJIA secrets higher amount of IL-1β and IL-18 while CAPS PBMCs produces more caspase-1 without stimulation. IL-1β, IL-18, and caspase-1 were significantly elevated among CAPS PBMCs (all p < 0.05) upon LPS stimulation, but not when additional ATPs were provided. Levels of cytokines and PBMC responses to the stimulation assays were similar among all sJIA patients regardless of their history of macrophage activation syndrome. Unstimulated PBMC activities and the LPS inflammasome stimulation assay without exogenic ATPs can assist the differentiation of CAPS from sJIA with persistent disease course. |
format | Online Article Text |
id | pubmed-8185076 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-81850762021-06-09 Lipopolysaccharide stimulation test on cultured PBMCs assists the discrimination of cryopyrin-associated periodic syndrome from systemic juvenile idiopathic arthritis Wu, Chao-Yi Fan, Wen-Lang Chiu, Ying-Ming Yang, Huang-Yu Lee, Wen-I. Huang, Jing-Long Sci Rep Article Systemic juvenile idiopathic arthritis (sJIA) and cryopyrin-associated periodic syndrome (CAPS) share many common manifestations. We aim to identify an applicable method to assist disease discrimination. Inflammatory cytokines were measured in the plasma of patients with CAPS, sJIA with persistent disease course and healthy controls. Supernatants collected from non-stimulated peripheral blood mononuclear cells (PBMCs) and those undergone inflammasome stimulation tests utilizing lipopolysaccharide (LPS) with and without adenosine triphosphate (ATP) were investigated. Inflammatory cytokines in patient plasma fail to differentiate sJIA from CAPS. PBMCs from sJIA secrets higher amount of IL-1β and IL-18 while CAPS PBMCs produces more caspase-1 without stimulation. IL-1β, IL-18, and caspase-1 were significantly elevated among CAPS PBMCs (all p < 0.05) upon LPS stimulation, but not when additional ATPs were provided. Levels of cytokines and PBMC responses to the stimulation assays were similar among all sJIA patients regardless of their history of macrophage activation syndrome. Unstimulated PBMC activities and the LPS inflammasome stimulation assay without exogenic ATPs can assist the differentiation of CAPS from sJIA with persistent disease course. Nature Publishing Group UK 2021-06-07 /pmc/articles/PMC8185076/ /pubmed/34099791 http://dx.doi.org/10.1038/s41598-021-91354-5 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Wu, Chao-Yi Fan, Wen-Lang Chiu, Ying-Ming Yang, Huang-Yu Lee, Wen-I. Huang, Jing-Long Lipopolysaccharide stimulation test on cultured PBMCs assists the discrimination of cryopyrin-associated periodic syndrome from systemic juvenile idiopathic arthritis |
title | Lipopolysaccharide stimulation test on cultured PBMCs assists the discrimination of cryopyrin-associated periodic syndrome from systemic juvenile idiopathic arthritis |
title_full | Lipopolysaccharide stimulation test on cultured PBMCs assists the discrimination of cryopyrin-associated periodic syndrome from systemic juvenile idiopathic arthritis |
title_fullStr | Lipopolysaccharide stimulation test on cultured PBMCs assists the discrimination of cryopyrin-associated periodic syndrome from systemic juvenile idiopathic arthritis |
title_full_unstemmed | Lipopolysaccharide stimulation test on cultured PBMCs assists the discrimination of cryopyrin-associated periodic syndrome from systemic juvenile idiopathic arthritis |
title_short | Lipopolysaccharide stimulation test on cultured PBMCs assists the discrimination of cryopyrin-associated periodic syndrome from systemic juvenile idiopathic arthritis |
title_sort | lipopolysaccharide stimulation test on cultured pbmcs assists the discrimination of cryopyrin-associated periodic syndrome from systemic juvenile idiopathic arthritis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185076/ https://www.ncbi.nlm.nih.gov/pubmed/34099791 http://dx.doi.org/10.1038/s41598-021-91354-5 |
work_keys_str_mv | AT wuchaoyi lipopolysaccharidestimulationtestonculturedpbmcsassiststhediscriminationofcryopyrinassociatedperiodicsyndromefromsystemicjuvenileidiopathicarthritis AT fanwenlang lipopolysaccharidestimulationtestonculturedpbmcsassiststhediscriminationofcryopyrinassociatedperiodicsyndromefromsystemicjuvenileidiopathicarthritis AT chiuyingming lipopolysaccharidestimulationtestonculturedpbmcsassiststhediscriminationofcryopyrinassociatedperiodicsyndromefromsystemicjuvenileidiopathicarthritis AT yanghuangyu lipopolysaccharidestimulationtestonculturedpbmcsassiststhediscriminationofcryopyrinassociatedperiodicsyndromefromsystemicjuvenileidiopathicarthritis AT leeweni lipopolysaccharidestimulationtestonculturedpbmcsassiststhediscriminationofcryopyrinassociatedperiodicsyndromefromsystemicjuvenileidiopathicarthritis AT huangjinglong lipopolysaccharidestimulationtestonculturedpbmcsassiststhediscriminationofcryopyrinassociatedperiodicsyndromefromsystemicjuvenileidiopathicarthritis |