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Nucleolar Phosphoprotein NPM1 Interacts With Porcine Circovirus Type 3 Cap Protein and Facilitates Viral Replication

Porcine circovirus type 3 (PCV3) is a recently discovered virus with potentially significant implications on the global swine industry. PCV3 replication involves the entry of the viral capsid (Cap) protein with nucleolar localization signals into the nucleus. Using liquid chromatography-mass spectro...

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Autores principales: Song, Jiangwei, Hou, Lei, Wang, Dan, Wei, Li, Zhu, Shanshan, Wang, Jing, Quan, Rong, Jiang, Haijun, Shi, Ruihan, Liu, Jue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185148/
https://www.ncbi.nlm.nih.gov/pubmed/34113334
http://dx.doi.org/10.3389/fmicb.2021.679341
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author Song, Jiangwei
Hou, Lei
Wang, Dan
Wei, Li
Zhu, Shanshan
Wang, Jing
Quan, Rong
Jiang, Haijun
Shi, Ruihan
Liu, Jue
author_facet Song, Jiangwei
Hou, Lei
Wang, Dan
Wei, Li
Zhu, Shanshan
Wang, Jing
Quan, Rong
Jiang, Haijun
Shi, Ruihan
Liu, Jue
author_sort Song, Jiangwei
collection PubMed
description Porcine circovirus type 3 (PCV3) is a recently discovered virus with potentially significant implications on the global swine industry. PCV3 replication involves the entry of the viral capsid (Cap) protein with nucleolar localization signals into the nucleus. Using liquid chromatography-mass spectrometry analysis, nucleolar phosphoprotein NPM1 was identified as one of the cellular proteins bound to PCV3 Cap. Co-immunoprecipitation demonstrated that PCV3 Cap interacts directly with NPM1, where the region binding with NPM1 is mapped to amino acid residues 1–38 of Cap. Upon co-transfection, the expression of Cap protein promoted the redistribution of NPM1, which translocated from the nucleus to the cytoplasm and colocalized with Cap in cultured PK15 cells. NPM1 expression was upregulated and translocated from the nucleus to the cytoplasm in PCV3-infected cells, upon siRNA-mediated depletion, or upon treatment with NPM1 inhibitor in PK15 cells with impaired PCV3 replication, as evidenced by decreased levels of viral DNA synthesis and protein expression. By contrast, the replication of PCV3 was enhanced in stably NPM1-expressing cells via a lentivirus-delivered system. Taken together, these findings indicate that NPM1 interacts with PCV3 Cap and plays a crucial role in PCV3 replication.
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spelling pubmed-81851482021-06-09 Nucleolar Phosphoprotein NPM1 Interacts With Porcine Circovirus Type 3 Cap Protein and Facilitates Viral Replication Song, Jiangwei Hou, Lei Wang, Dan Wei, Li Zhu, Shanshan Wang, Jing Quan, Rong Jiang, Haijun Shi, Ruihan Liu, Jue Front Microbiol Microbiology Porcine circovirus type 3 (PCV3) is a recently discovered virus with potentially significant implications on the global swine industry. PCV3 replication involves the entry of the viral capsid (Cap) protein with nucleolar localization signals into the nucleus. Using liquid chromatography-mass spectrometry analysis, nucleolar phosphoprotein NPM1 was identified as one of the cellular proteins bound to PCV3 Cap. Co-immunoprecipitation demonstrated that PCV3 Cap interacts directly with NPM1, where the region binding with NPM1 is mapped to amino acid residues 1–38 of Cap. Upon co-transfection, the expression of Cap protein promoted the redistribution of NPM1, which translocated from the nucleus to the cytoplasm and colocalized with Cap in cultured PK15 cells. NPM1 expression was upregulated and translocated from the nucleus to the cytoplasm in PCV3-infected cells, upon siRNA-mediated depletion, or upon treatment with NPM1 inhibitor in PK15 cells with impaired PCV3 replication, as evidenced by decreased levels of viral DNA synthesis and protein expression. By contrast, the replication of PCV3 was enhanced in stably NPM1-expressing cells via a lentivirus-delivered system. Taken together, these findings indicate that NPM1 interacts with PCV3 Cap and plays a crucial role in PCV3 replication. Frontiers Media S.A. 2021-05-25 /pmc/articles/PMC8185148/ /pubmed/34113334 http://dx.doi.org/10.3389/fmicb.2021.679341 Text en Copyright © 2021 Song, Hou, Wang, Wei, Zhu, Wang, Quan, Jiang, Shi and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Song, Jiangwei
Hou, Lei
Wang, Dan
Wei, Li
Zhu, Shanshan
Wang, Jing
Quan, Rong
Jiang, Haijun
Shi, Ruihan
Liu, Jue
Nucleolar Phosphoprotein NPM1 Interacts With Porcine Circovirus Type 3 Cap Protein and Facilitates Viral Replication
title Nucleolar Phosphoprotein NPM1 Interacts With Porcine Circovirus Type 3 Cap Protein and Facilitates Viral Replication
title_full Nucleolar Phosphoprotein NPM1 Interacts With Porcine Circovirus Type 3 Cap Protein and Facilitates Viral Replication
title_fullStr Nucleolar Phosphoprotein NPM1 Interacts With Porcine Circovirus Type 3 Cap Protein and Facilitates Viral Replication
title_full_unstemmed Nucleolar Phosphoprotein NPM1 Interacts With Porcine Circovirus Type 3 Cap Protein and Facilitates Viral Replication
title_short Nucleolar Phosphoprotein NPM1 Interacts With Porcine Circovirus Type 3 Cap Protein and Facilitates Viral Replication
title_sort nucleolar phosphoprotein npm1 interacts with porcine circovirus type 3 cap protein and facilitates viral replication
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185148/
https://www.ncbi.nlm.nih.gov/pubmed/34113334
http://dx.doi.org/10.3389/fmicb.2021.679341
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