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Consequences of Mixotrophy on Cell Energetic Metabolism in Microchloropsis gaditana Revealed by Genetic Engineering and Metabolic Approaches

Algae belonging to the Microchloropsis genus are promising organisms for biotech purposes, being able to accumulate large amounts of lipid reserves. These organisms adapt to different trophic conditions, thriving in strict photoautotrophic conditions, as well as in the concomitant presence of light...

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Autores principales: Bo, Davide Dal, Magneschi, Leonardo, Bedhomme, Mariette, Billey, Elodie, Deragon, Etienne, Storti, Mattia, Menneteau, Mathilde, Richard, Christelle, Rak, Camille, Lapeyre, Morgane, Lembrouk, Mehdi, Conte, Melissa, Gros, Valérie, Tourcier, Guillaume, Giustini, Cécile, Falconet, Denis, Curien, Gilles, Allorent, Guillaume, Petroutsos, Dimitris, Laeuffer, Frédéric, Fourage, Laurent, Jouhet, Juliette, Maréchal, Eric, Finazzi, Giovanni, Collin, Séverine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185151/
https://www.ncbi.nlm.nih.gov/pubmed/34113360
http://dx.doi.org/10.3389/fpls.2021.628684
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author Bo, Davide Dal
Magneschi, Leonardo
Bedhomme, Mariette
Billey, Elodie
Deragon, Etienne
Storti, Mattia
Menneteau, Mathilde
Richard, Christelle
Rak, Camille
Lapeyre, Morgane
Lembrouk, Mehdi
Conte, Melissa
Gros, Valérie
Tourcier, Guillaume
Giustini, Cécile
Falconet, Denis
Curien, Gilles
Allorent, Guillaume
Petroutsos, Dimitris
Laeuffer, Frédéric
Fourage, Laurent
Jouhet, Juliette
Maréchal, Eric
Finazzi, Giovanni
Collin, Séverine
author_facet Bo, Davide Dal
Magneschi, Leonardo
Bedhomme, Mariette
Billey, Elodie
Deragon, Etienne
Storti, Mattia
Menneteau, Mathilde
Richard, Christelle
Rak, Camille
Lapeyre, Morgane
Lembrouk, Mehdi
Conte, Melissa
Gros, Valérie
Tourcier, Guillaume
Giustini, Cécile
Falconet, Denis
Curien, Gilles
Allorent, Guillaume
Petroutsos, Dimitris
Laeuffer, Frédéric
Fourage, Laurent
Jouhet, Juliette
Maréchal, Eric
Finazzi, Giovanni
Collin, Séverine
author_sort Bo, Davide Dal
collection PubMed
description Algae belonging to the Microchloropsis genus are promising organisms for biotech purposes, being able to accumulate large amounts of lipid reserves. These organisms adapt to different trophic conditions, thriving in strict photoautotrophic conditions, as well as in the concomitant presence of light plus reduced external carbon as energy sources (mixotrophy). In this work, we investigated the mixotrophic responses of Microchloropsis gaditana (formerly Nannochloropsis gaditana). Using the Biolog growth test, in which cells are loaded into multiwell plates coated with different organic compounds, we could not find a suitable substrate for Microchloropsis mixotrophy. By contrast, addition of the Lysogeny broth (LB) to the inorganic growth medium had a benefit on growth, enhancing respiratory activity at the expense of photosynthetic performances. To further dissect the role of respiration in Microchloropsis mixotrophy, we focused on the mitochondrial alternative oxidase (AOX), a protein involved in energy management in other algae prospering in mixotrophy. Knocking-out the AOX1 gene by transcription activator-like effector nuclease (TALE-N) led to the loss of capacity to implement growth upon addition of LB supporting the hypothesis that the effect of this medium was related to a provision of reduced carbon. We conclude that mixotrophic growth in Microchloropsis is dominated by respiratory rather than by photosynthetic energetic metabolism and discuss the possible reasons for this behavior in relationship with fatty acid breakdown via β-oxidation in this oleaginous alga.
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spelling pubmed-81851512021-06-09 Consequences of Mixotrophy on Cell Energetic Metabolism in Microchloropsis gaditana Revealed by Genetic Engineering and Metabolic Approaches Bo, Davide Dal Magneschi, Leonardo Bedhomme, Mariette Billey, Elodie Deragon, Etienne Storti, Mattia Menneteau, Mathilde Richard, Christelle Rak, Camille Lapeyre, Morgane Lembrouk, Mehdi Conte, Melissa Gros, Valérie Tourcier, Guillaume Giustini, Cécile Falconet, Denis Curien, Gilles Allorent, Guillaume Petroutsos, Dimitris Laeuffer, Frédéric Fourage, Laurent Jouhet, Juliette Maréchal, Eric Finazzi, Giovanni Collin, Séverine Front Plant Sci Plant Science Algae belonging to the Microchloropsis genus are promising organisms for biotech purposes, being able to accumulate large amounts of lipid reserves. These organisms adapt to different trophic conditions, thriving in strict photoautotrophic conditions, as well as in the concomitant presence of light plus reduced external carbon as energy sources (mixotrophy). In this work, we investigated the mixotrophic responses of Microchloropsis gaditana (formerly Nannochloropsis gaditana). Using the Biolog growth test, in which cells are loaded into multiwell plates coated with different organic compounds, we could not find a suitable substrate for Microchloropsis mixotrophy. By contrast, addition of the Lysogeny broth (LB) to the inorganic growth medium had a benefit on growth, enhancing respiratory activity at the expense of photosynthetic performances. To further dissect the role of respiration in Microchloropsis mixotrophy, we focused on the mitochondrial alternative oxidase (AOX), a protein involved in energy management in other algae prospering in mixotrophy. Knocking-out the AOX1 gene by transcription activator-like effector nuclease (TALE-N) led to the loss of capacity to implement growth upon addition of LB supporting the hypothesis that the effect of this medium was related to a provision of reduced carbon. We conclude that mixotrophic growth in Microchloropsis is dominated by respiratory rather than by photosynthetic energetic metabolism and discuss the possible reasons for this behavior in relationship with fatty acid breakdown via β-oxidation in this oleaginous alga. Frontiers Media S.A. 2021-05-25 /pmc/articles/PMC8185151/ /pubmed/34113360 http://dx.doi.org/10.3389/fpls.2021.628684 Text en Copyright © 2021 Bo, Magneschi, Bedhomme, Billey, Deragon, Storti, Menneteau, Richard, Rak, Lapeyre, Lembrouk, Conte, Gros, Tourcier, Giustini, Falconet, Curien, Allorent, Petroutsos, Laeuffer, Fourage, Jouhet, Maréchal, Finazzi and Collin. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Bo, Davide Dal
Magneschi, Leonardo
Bedhomme, Mariette
Billey, Elodie
Deragon, Etienne
Storti, Mattia
Menneteau, Mathilde
Richard, Christelle
Rak, Camille
Lapeyre, Morgane
Lembrouk, Mehdi
Conte, Melissa
Gros, Valérie
Tourcier, Guillaume
Giustini, Cécile
Falconet, Denis
Curien, Gilles
Allorent, Guillaume
Petroutsos, Dimitris
Laeuffer, Frédéric
Fourage, Laurent
Jouhet, Juliette
Maréchal, Eric
Finazzi, Giovanni
Collin, Séverine
Consequences of Mixotrophy on Cell Energetic Metabolism in Microchloropsis gaditana Revealed by Genetic Engineering and Metabolic Approaches
title Consequences of Mixotrophy on Cell Energetic Metabolism in Microchloropsis gaditana Revealed by Genetic Engineering and Metabolic Approaches
title_full Consequences of Mixotrophy on Cell Energetic Metabolism in Microchloropsis gaditana Revealed by Genetic Engineering and Metabolic Approaches
title_fullStr Consequences of Mixotrophy on Cell Energetic Metabolism in Microchloropsis gaditana Revealed by Genetic Engineering and Metabolic Approaches
title_full_unstemmed Consequences of Mixotrophy on Cell Energetic Metabolism in Microchloropsis gaditana Revealed by Genetic Engineering and Metabolic Approaches
title_short Consequences of Mixotrophy on Cell Energetic Metabolism in Microchloropsis gaditana Revealed by Genetic Engineering and Metabolic Approaches
title_sort consequences of mixotrophy on cell energetic metabolism in microchloropsis gaditana revealed by genetic engineering and metabolic approaches
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185151/
https://www.ncbi.nlm.nih.gov/pubmed/34113360
http://dx.doi.org/10.3389/fpls.2021.628684
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