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Overexpression of a SOC1-Related Gene Promotes Bud Break in Ecodormant Poplars

Perennial species in the boreal and temperate regions are subject to extreme annual variations in light and temperature. They precisely adapt to seasonal changes by synchronizing cycles of growth and dormancy with external cues. Annual dormancy–growth transitions and flowering involve factors that i...

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Autores principales: Gómez-Soto, Daniela, Ramos-Sánchez, José M., Alique, Daniel, Conde, Daniel, Triozzi, Paolo M., Perales, Mariano, Allona, Isabel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185274/
https://www.ncbi.nlm.nih.gov/pubmed/34113369
http://dx.doi.org/10.3389/fpls.2021.670497
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author Gómez-Soto, Daniela
Ramos-Sánchez, José M.
Alique, Daniel
Conde, Daniel
Triozzi, Paolo M.
Perales, Mariano
Allona, Isabel
author_facet Gómez-Soto, Daniela
Ramos-Sánchez, José M.
Alique, Daniel
Conde, Daniel
Triozzi, Paolo M.
Perales, Mariano
Allona, Isabel
author_sort Gómez-Soto, Daniela
collection PubMed
description Perennial species in the boreal and temperate regions are subject to extreme annual variations in light and temperature. They precisely adapt to seasonal changes by synchronizing cycles of growth and dormancy with external cues. Annual dormancy–growth transitions and flowering involve factors that integrate environmental and endogenous signals. MADS-box transcription factors have been extensively described in the regulation of Arabidopsis flowering. However, their participation in annual dormancy–growth transitions in trees is minimal. In this study, we investigate the function of MADS12, a Populus tremula × alba SUPPRESSOR OF CONSTANS OVEREXPRESSION 1 (SOC1)-related gene. Our gene expression analysis reveals that MADS12 displays lower mRNA levels during the winter than during early spring and mid-spring. Moreover, MADS12 activation depends on the fulfillment of the chilling requirement. Hybrid poplars overexpressing MADS12 show no differences in growth cessation and bud set, while ecodormant plants display an early bud break, indicating that MADS12 overexpression promotes bud growth reactivation. Comparative expression analysis of available bud break-promoting genes reveals that MADS12 overexpression downregulates the GIBBERELLINS 2 OXIDASE 4 (GA2ox4), a gene involved in gibberellin catabolism. Moreover, the mid-winter to mid-spring RNAseq profiling indicates that MADS12 and GA2ox4 show antagonistic expression during bud dormancy release. Our results support MADS12 participation in the reactivation of shoot meristem growth during ecodormancy and link MADS12 activation and GA2ox4 downregulation within the temporal events that lead to poplar bud break.
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spelling pubmed-81852742021-06-09 Overexpression of a SOC1-Related Gene Promotes Bud Break in Ecodormant Poplars Gómez-Soto, Daniela Ramos-Sánchez, José M. Alique, Daniel Conde, Daniel Triozzi, Paolo M. Perales, Mariano Allona, Isabel Front Plant Sci Plant Science Perennial species in the boreal and temperate regions are subject to extreme annual variations in light and temperature. They precisely adapt to seasonal changes by synchronizing cycles of growth and dormancy with external cues. Annual dormancy–growth transitions and flowering involve factors that integrate environmental and endogenous signals. MADS-box transcription factors have been extensively described in the regulation of Arabidopsis flowering. However, their participation in annual dormancy–growth transitions in trees is minimal. In this study, we investigate the function of MADS12, a Populus tremula × alba SUPPRESSOR OF CONSTANS OVEREXPRESSION 1 (SOC1)-related gene. Our gene expression analysis reveals that MADS12 displays lower mRNA levels during the winter than during early spring and mid-spring. Moreover, MADS12 activation depends on the fulfillment of the chilling requirement. Hybrid poplars overexpressing MADS12 show no differences in growth cessation and bud set, while ecodormant plants display an early bud break, indicating that MADS12 overexpression promotes bud growth reactivation. Comparative expression analysis of available bud break-promoting genes reveals that MADS12 overexpression downregulates the GIBBERELLINS 2 OXIDASE 4 (GA2ox4), a gene involved in gibberellin catabolism. Moreover, the mid-winter to mid-spring RNAseq profiling indicates that MADS12 and GA2ox4 show antagonistic expression during bud dormancy release. Our results support MADS12 participation in the reactivation of shoot meristem growth during ecodormancy and link MADS12 activation and GA2ox4 downregulation within the temporal events that lead to poplar bud break. Frontiers Media S.A. 2021-05-25 /pmc/articles/PMC8185274/ /pubmed/34113369 http://dx.doi.org/10.3389/fpls.2021.670497 Text en Copyright © 2021 Gómez-Soto, Ramos-Sánchez, Alique, Conde, Triozzi, Perales and Allona. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Gómez-Soto, Daniela
Ramos-Sánchez, José M.
Alique, Daniel
Conde, Daniel
Triozzi, Paolo M.
Perales, Mariano
Allona, Isabel
Overexpression of a SOC1-Related Gene Promotes Bud Break in Ecodormant Poplars
title Overexpression of a SOC1-Related Gene Promotes Bud Break in Ecodormant Poplars
title_full Overexpression of a SOC1-Related Gene Promotes Bud Break in Ecodormant Poplars
title_fullStr Overexpression of a SOC1-Related Gene Promotes Bud Break in Ecodormant Poplars
title_full_unstemmed Overexpression of a SOC1-Related Gene Promotes Bud Break in Ecodormant Poplars
title_short Overexpression of a SOC1-Related Gene Promotes Bud Break in Ecodormant Poplars
title_sort overexpression of a soc1-related gene promotes bud break in ecodormant poplars
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185274/
https://www.ncbi.nlm.nih.gov/pubmed/34113369
http://dx.doi.org/10.3389/fpls.2021.670497
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