Cargando…

Phagocytosis and Efferocytosis by Resident Macrophages in the Mouse Pancreas

The tissue microenvironment in the mouse pancreas has been shown to promote very different polarizations of resident macrophages with islet-resident macrophages displaying an inflammatory “M1” profile and macrophages in the exocrine tissue mostly displaying an alternatively activated “M2” profile. T...

Descripción completa

Detalles Bibliográficos
Autores principales: Parv, Kristel, Westerlund, Nestori, Merchant, Kevin, Komijani, Milad, Lindsay, Robin S., Christoffersson, Gustaf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185276/
https://www.ncbi.nlm.nih.gov/pubmed/34113315
http://dx.doi.org/10.3389/fendo.2021.606175
_version_ 1783704755473743872
author Parv, Kristel
Westerlund, Nestori
Merchant, Kevin
Komijani, Milad
Lindsay, Robin S.
Christoffersson, Gustaf
author_facet Parv, Kristel
Westerlund, Nestori
Merchant, Kevin
Komijani, Milad
Lindsay, Robin S.
Christoffersson, Gustaf
author_sort Parv, Kristel
collection PubMed
description The tissue microenvironment in the mouse pancreas has been shown to promote very different polarizations of resident macrophages with islet-resident macrophages displaying an inflammatory “M1” profile and macrophages in the exocrine tissue mostly displaying an alternatively activated “M2” profile. The impact of this polarization on tissue homeostasis and diabetes development is unclear. In this study, the ability of pancreas-resident macrophages to phagocyte bacterial and endogenous debris was investigated. Mouse endocrine and exocrine tissues were separated, and tissue-resident macrophages were isolated by magnetic immunolabeling. Isolated macrophages were subjected to flow cytometry for polarization markers and qPCR for phagocytosis-related genes. Functional in vitro investigations included phagocytosis and efferocytosis assays using pH-sensitive fluorescent bacterial particles and dead fluorescent neutrophils, respectively. Intravital confocal imaging of in situ phagocytosis and efferocytosis in the pancreas was used to confirm findings in vivo. Gene expression analysis revealed no significant overall difference in expression of most phagocytosis-related genes in islet-resident vs. exocrine-resident macrophages included in the analysis. In this study, pancreas-resident macrophages were shown to differ in their ability to phagocyte bacterial and endogenous debris depending on their microenvironment. This difference in abilities may be one of the factors polarizing islet-resident macrophages to an inflammatory state since phagocytosis has been found to imprint macrophage heterogeneity. It remains unclear if this difference has any implications in the development of islet dysfunction or autoimmunity.
format Online
Article
Text
id pubmed-8185276
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-81852762021-06-09 Phagocytosis and Efferocytosis by Resident Macrophages in the Mouse Pancreas Parv, Kristel Westerlund, Nestori Merchant, Kevin Komijani, Milad Lindsay, Robin S. Christoffersson, Gustaf Front Endocrinol (Lausanne) Endocrinology The tissue microenvironment in the mouse pancreas has been shown to promote very different polarizations of resident macrophages with islet-resident macrophages displaying an inflammatory “M1” profile and macrophages in the exocrine tissue mostly displaying an alternatively activated “M2” profile. The impact of this polarization on tissue homeostasis and diabetes development is unclear. In this study, the ability of pancreas-resident macrophages to phagocyte bacterial and endogenous debris was investigated. Mouse endocrine and exocrine tissues were separated, and tissue-resident macrophages were isolated by magnetic immunolabeling. Isolated macrophages were subjected to flow cytometry for polarization markers and qPCR for phagocytosis-related genes. Functional in vitro investigations included phagocytosis and efferocytosis assays using pH-sensitive fluorescent bacterial particles and dead fluorescent neutrophils, respectively. Intravital confocal imaging of in situ phagocytosis and efferocytosis in the pancreas was used to confirm findings in vivo. Gene expression analysis revealed no significant overall difference in expression of most phagocytosis-related genes in islet-resident vs. exocrine-resident macrophages included in the analysis. In this study, pancreas-resident macrophages were shown to differ in their ability to phagocyte bacterial and endogenous debris depending on their microenvironment. This difference in abilities may be one of the factors polarizing islet-resident macrophages to an inflammatory state since phagocytosis has been found to imprint macrophage heterogeneity. It remains unclear if this difference has any implications in the development of islet dysfunction or autoimmunity. Frontiers Media S.A. 2021-05-25 /pmc/articles/PMC8185276/ /pubmed/34113315 http://dx.doi.org/10.3389/fendo.2021.606175 Text en Copyright © 2021 Parv, Westerlund, Merchant, Komijani, Lindsay and Christoffersson https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Endocrinology
Parv, Kristel
Westerlund, Nestori
Merchant, Kevin
Komijani, Milad
Lindsay, Robin S.
Christoffersson, Gustaf
Phagocytosis and Efferocytosis by Resident Macrophages in the Mouse Pancreas
title Phagocytosis and Efferocytosis by Resident Macrophages in the Mouse Pancreas
title_full Phagocytosis and Efferocytosis by Resident Macrophages in the Mouse Pancreas
title_fullStr Phagocytosis and Efferocytosis by Resident Macrophages in the Mouse Pancreas
title_full_unstemmed Phagocytosis and Efferocytosis by Resident Macrophages in the Mouse Pancreas
title_short Phagocytosis and Efferocytosis by Resident Macrophages in the Mouse Pancreas
title_sort phagocytosis and efferocytosis by resident macrophages in the mouse pancreas
topic Endocrinology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185276/
https://www.ncbi.nlm.nih.gov/pubmed/34113315
http://dx.doi.org/10.3389/fendo.2021.606175
work_keys_str_mv AT parvkristel phagocytosisandefferocytosisbyresidentmacrophagesinthemousepancreas
AT westerlundnestori phagocytosisandefferocytosisbyresidentmacrophagesinthemousepancreas
AT merchantkevin phagocytosisandefferocytosisbyresidentmacrophagesinthemousepancreas
AT komijanimilad phagocytosisandefferocytosisbyresidentmacrophagesinthemousepancreas
AT lindsayrobins phagocytosisandefferocytosisbyresidentmacrophagesinthemousepancreas
AT christofferssongustaf phagocytosisandefferocytosisbyresidentmacrophagesinthemousepancreas