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Borealin directs recruitment of the CPC to oocyte chromosomes and movement to the microtubules
The chromosomes in the oocytes of many animals appear to promote bipolar spindle assembly. In Drosophila oocytes, spindle assembly requires the chromosome passenger complex (CPC), which consists of INCENP, Borealin, Survivin, and Aurora B. To determine what recruits the CPC to the chromosomes and it...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Rockefeller University Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185691/ https://www.ncbi.nlm.nih.gov/pubmed/33836043 http://dx.doi.org/10.1083/jcb.202006018 |
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author | Wang, Lin-Ing DeFosse, Tyler Jang, Janet K. Battaglia, Rachel A. Wagner, Victoria F. McKim, Kim S. |
author_facet | Wang, Lin-Ing DeFosse, Tyler Jang, Janet K. Battaglia, Rachel A. Wagner, Victoria F. McKim, Kim S. |
author_sort | Wang, Lin-Ing |
collection | PubMed |
description | The chromosomes in the oocytes of many animals appear to promote bipolar spindle assembly. In Drosophila oocytes, spindle assembly requires the chromosome passenger complex (CPC), which consists of INCENP, Borealin, Survivin, and Aurora B. To determine what recruits the CPC to the chromosomes and its role in spindle assembly, we developed a strategy to manipulate the function and localization of INCENP, which is critical for recruiting the Aurora B kinase. We found that an interaction between Borealin and the chromatin is crucial for the recruitment of the CPC to the chromosomes and is sufficient to build kinetochores and recruit spindle microtubules. HP1 colocalizes with the CPC on the chromosomes and together they move to the spindle microtubules. We propose that the Borealin interaction with HP1 promotes the movement of the CPC from the chromosomes to the microtubules. In addition, within the central spindle, rather than at the centromeres, the CPC and HP1 are required for homologous chromosome bi-orientation. |
format | Online Article Text |
id | pubmed-8185691 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-81856912021-12-07 Borealin directs recruitment of the CPC to oocyte chromosomes and movement to the microtubules Wang, Lin-Ing DeFosse, Tyler Jang, Janet K. Battaglia, Rachel A. Wagner, Victoria F. McKim, Kim S. J Cell Biol Article The chromosomes in the oocytes of many animals appear to promote bipolar spindle assembly. In Drosophila oocytes, spindle assembly requires the chromosome passenger complex (CPC), which consists of INCENP, Borealin, Survivin, and Aurora B. To determine what recruits the CPC to the chromosomes and its role in spindle assembly, we developed a strategy to manipulate the function and localization of INCENP, which is critical for recruiting the Aurora B kinase. We found that an interaction between Borealin and the chromatin is crucial for the recruitment of the CPC to the chromosomes and is sufficient to build kinetochores and recruit spindle microtubules. HP1 colocalizes with the CPC on the chromosomes and together they move to the spindle microtubules. We propose that the Borealin interaction with HP1 promotes the movement of the CPC from the chromosomes to the microtubules. In addition, within the central spindle, rather than at the centromeres, the CPC and HP1 are required for homologous chromosome bi-orientation. Rockefeller University Press 2021-04-09 /pmc/articles/PMC8185691/ /pubmed/33836043 http://dx.doi.org/10.1083/jcb.202006018 Text en © 2021 Wang et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Wang, Lin-Ing DeFosse, Tyler Jang, Janet K. Battaglia, Rachel A. Wagner, Victoria F. McKim, Kim S. Borealin directs recruitment of the CPC to oocyte chromosomes and movement to the microtubules |
title | Borealin directs recruitment of the CPC to oocyte chromosomes and movement to the microtubules |
title_full | Borealin directs recruitment of the CPC to oocyte chromosomes and movement to the microtubules |
title_fullStr | Borealin directs recruitment of the CPC to oocyte chromosomes and movement to the microtubules |
title_full_unstemmed | Borealin directs recruitment of the CPC to oocyte chromosomes and movement to the microtubules |
title_short | Borealin directs recruitment of the CPC to oocyte chromosomes and movement to the microtubules |
title_sort | borealin directs recruitment of the cpc to oocyte chromosomes and movement to the microtubules |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185691/ https://www.ncbi.nlm.nih.gov/pubmed/33836043 http://dx.doi.org/10.1083/jcb.202006018 |
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