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Evaluation on two types of paramyosin vaccines for the control of Haemaphysalislongicornis infestations in rabbits
BACKGROUND: Haemaphysalislongicornis is an obligate hematophagous ectoparasite that transmits a variety of pathogens causing life-threatening diseases in humans and animals. Paramyosin (Pmy) is not only an invertebrate-specific myofibrillar protein but also an important immunomodulatory protein. The...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185926/ https://www.ncbi.nlm.nih.gov/pubmed/34099029 http://dx.doi.org/10.1186/s13071-021-04812-4 |
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author | Wu, Pin-Xing Cui, Xue-Jiao Cao, Mi-Xue Lv, Li-Hong Dong, Hong-Meng Xiao, Shu-Wen Liu, Jing-Ze Hu, Yong-Hong |
author_facet | Wu, Pin-Xing Cui, Xue-Jiao Cao, Mi-Xue Lv, Li-Hong Dong, Hong-Meng Xiao, Shu-Wen Liu, Jing-Ze Hu, Yong-Hong |
author_sort | Wu, Pin-Xing |
collection | PubMed |
description | BACKGROUND: Haemaphysalislongicornis is an obligate hematophagous ectoparasite that transmits a variety of pathogens causing life-threatening diseases in humans and animals. Paramyosin (Pmy) is not only an invertebrate-specific myofibrillar protein but also an important immunomodulatory protein. Therefore, it is one of the ideal candidate antigens for vaccines. METHODS: We conducted two vaccine trials to evaluate the protective efficacy of Pmy recombinant protein (rPmy) and peptide vaccine (KLH-LEE). Each rabbit was immunized with three doses of rPmy or KLH-LEE adjuvanted with Freund’s complete/incomplete at 500 μg/dose at 2-week intervals before challenge with 40 female H. longicornis/rabbit. PBS plus adjuvant, Trx or KLH was used as control group. The antibodies of rabbits were detected by ELISA. Then, female ticks were fed on the rabbits until detachment. RESULTS: ELISA results showed that both vaccines induced rabbits to produce antibodies. Compared with the Trx group, the engorgement weight, oviposition and hatchability of the rPmy group decreased by 8.87%, 26.83% and 38.86%, respectively. On the other hand, engorgement weight, oviposition and hatchability of female ticks in the KLH-LEE group correspondingly resulted in 27.03%, 53.15% and 38.40% reduction compared with that of the KLH group. Considering the cumulative effect of vaccination on the evaluated parameters, results showed 60.37% efficacy of the rPmy vaccine formulation and 70.86% efficacy in the KLH-LEE group. CONCLUSIONS: Pmy and particularly epitope LEE have potential for further development of an effective candidate vaccine to protect the host against tick infection. GRAPHIC ABSTARCT: [Image: see text] |
format | Online Article Text |
id | pubmed-8185926 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-81859262021-06-09 Evaluation on two types of paramyosin vaccines for the control of Haemaphysalislongicornis infestations in rabbits Wu, Pin-Xing Cui, Xue-Jiao Cao, Mi-Xue Lv, Li-Hong Dong, Hong-Meng Xiao, Shu-Wen Liu, Jing-Ze Hu, Yong-Hong Parasit Vectors Short Report BACKGROUND: Haemaphysalislongicornis is an obligate hematophagous ectoparasite that transmits a variety of pathogens causing life-threatening diseases in humans and animals. Paramyosin (Pmy) is not only an invertebrate-specific myofibrillar protein but also an important immunomodulatory protein. Therefore, it is one of the ideal candidate antigens for vaccines. METHODS: We conducted two vaccine trials to evaluate the protective efficacy of Pmy recombinant protein (rPmy) and peptide vaccine (KLH-LEE). Each rabbit was immunized with three doses of rPmy or KLH-LEE adjuvanted with Freund’s complete/incomplete at 500 μg/dose at 2-week intervals before challenge with 40 female H. longicornis/rabbit. PBS plus adjuvant, Trx or KLH was used as control group. The antibodies of rabbits were detected by ELISA. Then, female ticks were fed on the rabbits until detachment. RESULTS: ELISA results showed that both vaccines induced rabbits to produce antibodies. Compared with the Trx group, the engorgement weight, oviposition and hatchability of the rPmy group decreased by 8.87%, 26.83% and 38.86%, respectively. On the other hand, engorgement weight, oviposition and hatchability of female ticks in the KLH-LEE group correspondingly resulted in 27.03%, 53.15% and 38.40% reduction compared with that of the KLH group. Considering the cumulative effect of vaccination on the evaluated parameters, results showed 60.37% efficacy of the rPmy vaccine formulation and 70.86% efficacy in the KLH-LEE group. CONCLUSIONS: Pmy and particularly epitope LEE have potential for further development of an effective candidate vaccine to protect the host against tick infection. GRAPHIC ABSTARCT: [Image: see text] BioMed Central 2021-06-07 /pmc/articles/PMC8185926/ /pubmed/34099029 http://dx.doi.org/10.1186/s13071-021-04812-4 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Short Report Wu, Pin-Xing Cui, Xue-Jiao Cao, Mi-Xue Lv, Li-Hong Dong, Hong-Meng Xiao, Shu-Wen Liu, Jing-Ze Hu, Yong-Hong Evaluation on two types of paramyosin vaccines for the control of Haemaphysalislongicornis infestations in rabbits |
title | Evaluation on two types of paramyosin vaccines for the control of Haemaphysalislongicornis infestations in rabbits |
title_full | Evaluation on two types of paramyosin vaccines for the control of Haemaphysalislongicornis infestations in rabbits |
title_fullStr | Evaluation on two types of paramyosin vaccines for the control of Haemaphysalislongicornis infestations in rabbits |
title_full_unstemmed | Evaluation on two types of paramyosin vaccines for the control of Haemaphysalislongicornis infestations in rabbits |
title_short | Evaluation on two types of paramyosin vaccines for the control of Haemaphysalislongicornis infestations in rabbits |
title_sort | evaluation on two types of paramyosin vaccines for the control of haemaphysalislongicornis infestations in rabbits |
topic | Short Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185926/ https://www.ncbi.nlm.nih.gov/pubmed/34099029 http://dx.doi.org/10.1186/s13071-021-04812-4 |
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