Cargando…
Pulmonary hydatidosis genotypes isolates from human clinical surgery based on sequencing of mitochondrial genes in Fars, Iran
BACKGROUND: Cystic echinococcosis (CE)/hydatidosis is an important neglected parasitic zoonotic disease caused by the metacestode of Echinococcus granulosus s.l. The present study was designed to identify the pulmonary CE species/genotypes in isolated human underwent to surgery in our center in Sout...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8186107/ https://www.ncbi.nlm.nih.gov/pubmed/34099002 http://dx.doi.org/10.1186/s13019-021-01547-2 |
_version_ | 1783704895324422144 |
---|---|
author | Mardani, Parviz Ezabadi, Ali Talebi Sedaghat, Bahareh Sadjjadi, Seyed Mahmoud |
author_facet | Mardani, Parviz Ezabadi, Ali Talebi Sedaghat, Bahareh Sadjjadi, Seyed Mahmoud |
author_sort | Mardani, Parviz |
collection | PubMed |
description | BACKGROUND: Cystic echinococcosis (CE)/hydatidosis is an important neglected parasitic zoonotic disease caused by the metacestode of Echinococcus granulosus s.l. The present study was designed to identify the pulmonary CE species/genotypes in isolated human underwent to surgery in our center in Southern Iran. METHODS: The study population of this study were all patients in Fars province who were admitted to Namazi Hospitals for pulmonary hydatid cyst surgery. Thoracic surgery was performed in the thoracic ward and the cyst/s was removed by open surgery via posterolateral or lateral thoracotomy. DNA was extracted from the germinal layer or the protoscoleces. PCR technique was performed using the cytochrome C oxidase subunit1 (cox1) gene, and the products were sequenced. RESULTS: A total of 32 pulmonary hydatid cyst samples were collected from 9 (28%) female and 23 (72%) male aged from 4 to 74 years old. A total of 18(56%) cyst/s were in the left lobe and 14 (44%) cysts in the right lobe. Sequence analysis of the cysts showed that 24 samples (75%) were E. granulosus s.s (G1-G3) genotype and 8 (25%) were E. canadensis (G6/G7) genotype. CONCLUSION: E.granulosus s.s genotype was the most prevalent genotype followed by E. canadensis (G6/G7) genotype. There was no significant statistical correlation between cysts’ size, location, genotype strain, and patients’ age and gender. |
format | Online Article Text |
id | pubmed-8186107 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-81861072021-06-10 Pulmonary hydatidosis genotypes isolates from human clinical surgery based on sequencing of mitochondrial genes in Fars, Iran Mardani, Parviz Ezabadi, Ali Talebi Sedaghat, Bahareh Sadjjadi, Seyed Mahmoud J Cardiothorac Surg Research Article BACKGROUND: Cystic echinococcosis (CE)/hydatidosis is an important neglected parasitic zoonotic disease caused by the metacestode of Echinococcus granulosus s.l. The present study was designed to identify the pulmonary CE species/genotypes in isolated human underwent to surgery in our center in Southern Iran. METHODS: The study population of this study were all patients in Fars province who were admitted to Namazi Hospitals for pulmonary hydatid cyst surgery. Thoracic surgery was performed in the thoracic ward and the cyst/s was removed by open surgery via posterolateral or lateral thoracotomy. DNA was extracted from the germinal layer or the protoscoleces. PCR technique was performed using the cytochrome C oxidase subunit1 (cox1) gene, and the products were sequenced. RESULTS: A total of 32 pulmonary hydatid cyst samples were collected from 9 (28%) female and 23 (72%) male aged from 4 to 74 years old. A total of 18(56%) cyst/s were in the left lobe and 14 (44%) cysts in the right lobe. Sequence analysis of the cysts showed that 24 samples (75%) were E. granulosus s.s (G1-G3) genotype and 8 (25%) were E. canadensis (G6/G7) genotype. CONCLUSION: E.granulosus s.s genotype was the most prevalent genotype followed by E. canadensis (G6/G7) genotype. There was no significant statistical correlation between cysts’ size, location, genotype strain, and patients’ age and gender. BioMed Central 2021-06-07 /pmc/articles/PMC8186107/ /pubmed/34099002 http://dx.doi.org/10.1186/s13019-021-01547-2 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Mardani, Parviz Ezabadi, Ali Talebi Sedaghat, Bahareh Sadjjadi, Seyed Mahmoud Pulmonary hydatidosis genotypes isolates from human clinical surgery based on sequencing of mitochondrial genes in Fars, Iran |
title | Pulmonary hydatidosis genotypes isolates from human clinical surgery based on sequencing of mitochondrial genes in Fars, Iran |
title_full | Pulmonary hydatidosis genotypes isolates from human clinical surgery based on sequencing of mitochondrial genes in Fars, Iran |
title_fullStr | Pulmonary hydatidosis genotypes isolates from human clinical surgery based on sequencing of mitochondrial genes in Fars, Iran |
title_full_unstemmed | Pulmonary hydatidosis genotypes isolates from human clinical surgery based on sequencing of mitochondrial genes in Fars, Iran |
title_short | Pulmonary hydatidosis genotypes isolates from human clinical surgery based on sequencing of mitochondrial genes in Fars, Iran |
title_sort | pulmonary hydatidosis genotypes isolates from human clinical surgery based on sequencing of mitochondrial genes in fars, iran |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8186107/ https://www.ncbi.nlm.nih.gov/pubmed/34099002 http://dx.doi.org/10.1186/s13019-021-01547-2 |
work_keys_str_mv | AT mardaniparviz pulmonaryhydatidosisgenotypesisolatesfromhumanclinicalsurgerybasedonsequencingofmitochondrialgenesinfarsiran AT ezabadialitalebi pulmonaryhydatidosisgenotypesisolatesfromhumanclinicalsurgerybasedonsequencingofmitochondrialgenesinfarsiran AT sedaghatbahareh pulmonaryhydatidosisgenotypesisolatesfromhumanclinicalsurgerybasedonsequencingofmitochondrialgenesinfarsiran AT sadjjadiseyedmahmoud pulmonaryhydatidosisgenotypesisolatesfromhumanclinicalsurgerybasedonsequencingofmitochondrialgenesinfarsiran |