Cargando…
Implication of Toll/IL-1 receptor domain containing adapters in Porphyromonas gingivalis-induced inflammation
Periodontitis is induced by periodontal dysbiosis characterized by the predominance of anaerobic species. TLRs constitute the classical pathway for cell activation by infection. Interestingly, the Toll/IL-1 receptor homology domain adapters initiate signaling events, leading to the activation of the...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8186158/ https://www.ncbi.nlm.nih.gov/pubmed/34018827 http://dx.doi.org/10.1177/17534259211013087 |
_version_ | 1783704906144677888 |
---|---|
author | Bugueno, Isaac M Benkirane-Jessel, Nadia Huck, Olivier |
author_facet | Bugueno, Isaac M Benkirane-Jessel, Nadia Huck, Olivier |
author_sort | Bugueno, Isaac M |
collection | PubMed |
description | Periodontitis is induced by periodontal dysbiosis characterized by the predominance of anaerobic species. TLRs constitute the classical pathway for cell activation by infection. Interestingly, the Toll/IL-1 receptor homology domain adapters initiate signaling events, leading to the activation of the expression of the genes involved in the host immune response. The aim of this study was to evaluate the effects of Porphyromonas gingivalis on the expression and protein-protein interactions among five TIR adapters (MAL, MyD88, TRIF, TRAM and SARM) in gingival epithelial cells and endothelial cells. It was observed that P. gingivalis is able to modulate the signaling cascades activated through its recognition by TLR4/2 in gingival epithelial cells and endothelial cells. Indeed, MAL-MyD88 protein-protein interactions associated with TLR4 was the main pathway activated by P. gingivalis infection. When transient siRNA inhibition was performed, cell viability, inflammation, and cell death induced by infection decreased and such deleterious effects were almost absent when MAL or TRAM were targeted. This study emphasizes the role of such TIR adapter proteins in P. gingivalis elicited inflammation and the precise evaluation of TIR adapter protein interactions may pave the way for future therapeutics in both periodontitis and systemic disease with a P. gingivalis involvement, such as atherothrombosis. |
format | Online Article Text |
id | pubmed-8186158 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-81861582021-06-21 Implication of Toll/IL-1 receptor domain containing adapters in Porphyromonas gingivalis-induced inflammation Bugueno, Isaac M Benkirane-Jessel, Nadia Huck, Olivier Innate Immun Original Articles Periodontitis is induced by periodontal dysbiosis characterized by the predominance of anaerobic species. TLRs constitute the classical pathway for cell activation by infection. Interestingly, the Toll/IL-1 receptor homology domain adapters initiate signaling events, leading to the activation of the expression of the genes involved in the host immune response. The aim of this study was to evaluate the effects of Porphyromonas gingivalis on the expression and protein-protein interactions among five TIR adapters (MAL, MyD88, TRIF, TRAM and SARM) in gingival epithelial cells and endothelial cells. It was observed that P. gingivalis is able to modulate the signaling cascades activated through its recognition by TLR4/2 in gingival epithelial cells and endothelial cells. Indeed, MAL-MyD88 protein-protein interactions associated with TLR4 was the main pathway activated by P. gingivalis infection. When transient siRNA inhibition was performed, cell viability, inflammation, and cell death induced by infection decreased and such deleterious effects were almost absent when MAL or TRAM were targeted. This study emphasizes the role of such TIR adapter proteins in P. gingivalis elicited inflammation and the precise evaluation of TIR adapter protein interactions may pave the way for future therapeutics in both periodontitis and systemic disease with a P. gingivalis involvement, such as atherothrombosis. SAGE Publications 2021-05-21 2021-05 /pmc/articles/PMC8186158/ /pubmed/34018827 http://dx.doi.org/10.1177/17534259211013087 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by-nc/4.0/Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Original Articles Bugueno, Isaac M Benkirane-Jessel, Nadia Huck, Olivier Implication of Toll/IL-1 receptor domain containing adapters in Porphyromonas gingivalis-induced inflammation |
title | Implication of Toll/IL-1 receptor domain containing adapters in Porphyromonas gingivalis-induced inflammation |
title_full | Implication of Toll/IL-1 receptor domain containing adapters in Porphyromonas gingivalis-induced inflammation |
title_fullStr | Implication of Toll/IL-1 receptor domain containing adapters in Porphyromonas gingivalis-induced inflammation |
title_full_unstemmed | Implication of Toll/IL-1 receptor domain containing adapters in Porphyromonas gingivalis-induced inflammation |
title_short | Implication of Toll/IL-1 receptor domain containing adapters in Porphyromonas gingivalis-induced inflammation |
title_sort | implication of toll/il-1 receptor domain containing adapters in porphyromonas gingivalis-induced inflammation |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8186158/ https://www.ncbi.nlm.nih.gov/pubmed/34018827 http://dx.doi.org/10.1177/17534259211013087 |
work_keys_str_mv | AT buguenoisaacm implicationoftollil1receptordomaincontainingadaptersinporphyromonasgingivalisinducedinflammation AT benkiranejesselnadia implicationoftollil1receptordomaincontainingadaptersinporphyromonasgingivalisinducedinflammation AT huckolivier implicationoftollil1receptordomaincontainingadaptersinporphyromonasgingivalisinducedinflammation |