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Implication of Toll/IL-1 receptor domain containing adapters in Porphyromonas gingivalis-induced inflammation

Periodontitis is induced by periodontal dysbiosis characterized by the predominance of anaerobic species. TLRs constitute the classical pathway for cell activation by infection. Interestingly, the Toll/IL-1 receptor homology domain adapters initiate signaling events, leading to the activation of the...

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Autores principales: Bugueno, Isaac M, Benkirane-Jessel, Nadia, Huck, Olivier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8186158/
https://www.ncbi.nlm.nih.gov/pubmed/34018827
http://dx.doi.org/10.1177/17534259211013087
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author Bugueno, Isaac M
Benkirane-Jessel, Nadia
Huck, Olivier
author_facet Bugueno, Isaac M
Benkirane-Jessel, Nadia
Huck, Olivier
author_sort Bugueno, Isaac M
collection PubMed
description Periodontitis is induced by periodontal dysbiosis characterized by the predominance of anaerobic species. TLRs constitute the classical pathway for cell activation by infection. Interestingly, the Toll/IL-1 receptor homology domain adapters initiate signaling events, leading to the activation of the expression of the genes involved in the host immune response. The aim of this study was to evaluate the effects of Porphyromonas gingivalis on the expression and protein-protein interactions among five TIR adapters (MAL, MyD88, TRIF, TRAM and SARM) in gingival epithelial cells and endothelial cells. It was observed that P. gingivalis is able to modulate the signaling cascades activated through its recognition by TLR4/2 in gingival epithelial cells and endothelial cells. Indeed, MAL-MyD88 protein-protein interactions associated with TLR4 was the main pathway activated by P. gingivalis infection. When transient siRNA inhibition was performed, cell viability, inflammation, and cell death induced by infection decreased and such deleterious effects were almost absent when MAL or TRAM were targeted. This study emphasizes the role of such TIR adapter proteins in P. gingivalis elicited inflammation and the precise evaluation of TIR adapter protein interactions may pave the way for future therapeutics in both periodontitis and systemic disease with a P. gingivalis involvement, such as atherothrombosis.
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spelling pubmed-81861582021-06-21 Implication of Toll/IL-1 receptor domain containing adapters in Porphyromonas gingivalis-induced inflammation Bugueno, Isaac M Benkirane-Jessel, Nadia Huck, Olivier Innate Immun Original Articles Periodontitis is induced by periodontal dysbiosis characterized by the predominance of anaerobic species. TLRs constitute the classical pathway for cell activation by infection. Interestingly, the Toll/IL-1 receptor homology domain adapters initiate signaling events, leading to the activation of the expression of the genes involved in the host immune response. The aim of this study was to evaluate the effects of Porphyromonas gingivalis on the expression and protein-protein interactions among five TIR adapters (MAL, MyD88, TRIF, TRAM and SARM) in gingival epithelial cells and endothelial cells. It was observed that P. gingivalis is able to modulate the signaling cascades activated through its recognition by TLR4/2 in gingival epithelial cells and endothelial cells. Indeed, MAL-MyD88 protein-protein interactions associated with TLR4 was the main pathway activated by P. gingivalis infection. When transient siRNA inhibition was performed, cell viability, inflammation, and cell death induced by infection decreased and such deleterious effects were almost absent when MAL or TRAM were targeted. This study emphasizes the role of such TIR adapter proteins in P. gingivalis elicited inflammation and the precise evaluation of TIR adapter protein interactions may pave the way for future therapeutics in both periodontitis and systemic disease with a P. gingivalis involvement, such as atherothrombosis. SAGE Publications 2021-05-21 2021-05 /pmc/articles/PMC8186158/ /pubmed/34018827 http://dx.doi.org/10.1177/17534259211013087 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by-nc/4.0/Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Articles
Bugueno, Isaac M
Benkirane-Jessel, Nadia
Huck, Olivier
Implication of Toll/IL-1 receptor domain containing adapters in Porphyromonas gingivalis-induced inflammation
title Implication of Toll/IL-1 receptor domain containing adapters in Porphyromonas gingivalis-induced inflammation
title_full Implication of Toll/IL-1 receptor domain containing adapters in Porphyromonas gingivalis-induced inflammation
title_fullStr Implication of Toll/IL-1 receptor domain containing adapters in Porphyromonas gingivalis-induced inflammation
title_full_unstemmed Implication of Toll/IL-1 receptor domain containing adapters in Porphyromonas gingivalis-induced inflammation
title_short Implication of Toll/IL-1 receptor domain containing adapters in Porphyromonas gingivalis-induced inflammation
title_sort implication of toll/il-1 receptor domain containing adapters in porphyromonas gingivalis-induced inflammation
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8186158/
https://www.ncbi.nlm.nih.gov/pubmed/34018827
http://dx.doi.org/10.1177/17534259211013087
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