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The Presence of Circulating Nucleated Red Blood Cells Is Associated With Disease Severity in Patients of Hemorrhagic Fever With Renal Syndrome

Hemorrhagic fever with renal syndrome (HFRS) is a regional infectious disease of epidemic potential caused by the Hantaan virus (HTNV). Red blood cells (RBCs) are the major components of peripheral blood. However, pathological changes in RBCs and the underlying mechanisms during HTNV infection remai...

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Autores principales: Zhang, Jingang, Tang, Kang, Zhang, Yun, Ma, Ying, Zhang, Chunmei, Hu, Haifeng, Jia, Xiaozhou, Zhuang, Ran, Jin, Boquan, Wang, Meng, Zhang, Xiyue, Liu, Dalu, Zhang, Yusi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8186265/
https://www.ncbi.nlm.nih.gov/pubmed/34113638
http://dx.doi.org/10.3389/fmed.2021.665410
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author Zhang, Jingang
Tang, Kang
Zhang, Yun
Ma, Ying
Zhang, Chunmei
Hu, Haifeng
Jia, Xiaozhou
Zhuang, Ran
Jin, Boquan
Wang, Meng
Zhang, Xiyue
Liu, Dalu
Zhang, Yusi
author_facet Zhang, Jingang
Tang, Kang
Zhang, Yun
Ma, Ying
Zhang, Chunmei
Hu, Haifeng
Jia, Xiaozhou
Zhuang, Ran
Jin, Boquan
Wang, Meng
Zhang, Xiyue
Liu, Dalu
Zhang, Yusi
author_sort Zhang, Jingang
collection PubMed
description Hemorrhagic fever with renal syndrome (HFRS) is a regional infectious disease of epidemic potential caused by the Hantaan virus (HTNV). Red blood cells (RBCs) are the major components of peripheral blood. However, pathological changes in RBCs and the underlying mechanisms during HTNV infection remain largely unclear. Therefore, this study sought to explore changes in RBCs in the peripheral blood of HFRS patients. We isolated PBMCs from HFRS patients and performed single-cell RNA sequencing. The results showed that clusters of RBCs in the peripheral blood of HFRS could be classified as nucleated red blood cells (NRBC) based on their cellular components, gene expression profiles and cell surface markers. In addition, it was shown that the higher the count of NRBC in peripheral blood, the more severe the disease status was. Moreover, hematological indices related to RBCs were analyzed and the results showed that impairment in the folate pathway might be the possible reason behind the presence of NRBCs. This study, for the first time showed that the presence of NRBCs in the peripheral blood of HFRS patients was associated with disease severity. This was also the first study to show that infection with the HTNV virus hindered the maturation of RBCs. Therefore, this work provides further insights on the role of and pathological changes in RBCs during HTNV infection.
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spelling pubmed-81862652021-06-09 The Presence of Circulating Nucleated Red Blood Cells Is Associated With Disease Severity in Patients of Hemorrhagic Fever With Renal Syndrome Zhang, Jingang Tang, Kang Zhang, Yun Ma, Ying Zhang, Chunmei Hu, Haifeng Jia, Xiaozhou Zhuang, Ran Jin, Boquan Wang, Meng Zhang, Xiyue Liu, Dalu Zhang, Yusi Front Med (Lausanne) Medicine Hemorrhagic fever with renal syndrome (HFRS) is a regional infectious disease of epidemic potential caused by the Hantaan virus (HTNV). Red blood cells (RBCs) are the major components of peripheral blood. However, pathological changes in RBCs and the underlying mechanisms during HTNV infection remain largely unclear. Therefore, this study sought to explore changes in RBCs in the peripheral blood of HFRS patients. We isolated PBMCs from HFRS patients and performed single-cell RNA sequencing. The results showed that clusters of RBCs in the peripheral blood of HFRS could be classified as nucleated red blood cells (NRBC) based on their cellular components, gene expression profiles and cell surface markers. In addition, it was shown that the higher the count of NRBC in peripheral blood, the more severe the disease status was. Moreover, hematological indices related to RBCs were analyzed and the results showed that impairment in the folate pathway might be the possible reason behind the presence of NRBCs. This study, for the first time showed that the presence of NRBCs in the peripheral blood of HFRS patients was associated with disease severity. This was also the first study to show that infection with the HTNV virus hindered the maturation of RBCs. Therefore, this work provides further insights on the role of and pathological changes in RBCs during HTNV infection. Frontiers Media S.A. 2021-05-25 /pmc/articles/PMC8186265/ /pubmed/34113638 http://dx.doi.org/10.3389/fmed.2021.665410 Text en Copyright © 2021 Zhang, Tang, Zhang, Ma, Zhang, Hu, Jia, Zhuang, Jin, Wang, Zhang, Liu and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Medicine
Zhang, Jingang
Tang, Kang
Zhang, Yun
Ma, Ying
Zhang, Chunmei
Hu, Haifeng
Jia, Xiaozhou
Zhuang, Ran
Jin, Boquan
Wang, Meng
Zhang, Xiyue
Liu, Dalu
Zhang, Yusi
The Presence of Circulating Nucleated Red Blood Cells Is Associated With Disease Severity in Patients of Hemorrhagic Fever With Renal Syndrome
title The Presence of Circulating Nucleated Red Blood Cells Is Associated With Disease Severity in Patients of Hemorrhagic Fever With Renal Syndrome
title_full The Presence of Circulating Nucleated Red Blood Cells Is Associated With Disease Severity in Patients of Hemorrhagic Fever With Renal Syndrome
title_fullStr The Presence of Circulating Nucleated Red Blood Cells Is Associated With Disease Severity in Patients of Hemorrhagic Fever With Renal Syndrome
title_full_unstemmed The Presence of Circulating Nucleated Red Blood Cells Is Associated With Disease Severity in Patients of Hemorrhagic Fever With Renal Syndrome
title_short The Presence of Circulating Nucleated Red Blood Cells Is Associated With Disease Severity in Patients of Hemorrhagic Fever With Renal Syndrome
title_sort presence of circulating nucleated red blood cells is associated with disease severity in patients of hemorrhagic fever with renal syndrome
topic Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8186265/
https://www.ncbi.nlm.nih.gov/pubmed/34113638
http://dx.doi.org/10.3389/fmed.2021.665410
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