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Oocyte maturation with royal jelly increases embryo development and reduces apoptosis in goats
Royal jelly (RJ) was supplemented to goat oocyte in vitro maturation (IVM) medium at three different concentrations (2.5, 5, and 10 mg/ml). Maturation rate, embryo cleavage, and blastocyst rate were recorded. Gene expression of apoptosis-related transcripts was investigated in matured oocytes. Perce...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Colégio Brasileiro de Reprodução Animal
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8186877/ https://www.ncbi.nlm.nih.gov/pubmed/34122643 http://dx.doi.org/10.21451/1984-3143-2017-AR986 |
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author | Veshkini, Arash Mohammadi-Sangcheshmeh, Abdollah Ghanem, Nasser Abazari-kia, Amir Hossein Mottaghi, Elmira Kamaledini, Reza Deldar, Hamid Ozturk, Irfan Gastal, Eduardo Leite |
author_facet | Veshkini, Arash Mohammadi-Sangcheshmeh, Abdollah Ghanem, Nasser Abazari-kia, Amir Hossein Mottaghi, Elmira Kamaledini, Reza Deldar, Hamid Ozturk, Irfan Gastal, Eduardo Leite |
author_sort | Veshkini, Arash |
collection | PubMed |
description | Royal jelly (RJ) was supplemented to goat oocyte in vitro maturation (IVM) medium at three different concentrations (2.5, 5, and 10 mg/ml). Maturation rate, embryo cleavage, and blastocyst rate were recorded. Gene expression of apoptosis-related transcripts was investigated in matured oocytes. Percentage of oocytes that reached MII-stage was increased in RJ-treated groups compared to the control group. Glutathione (GSH) content of mature oocytes was enhanced when RJ was added to IVM medium at any supplementation compared with control. Percentage of cleaved embryos and blastocysts was higher in the RJ-treated groups at a concentration of 5 than in the 2.5 mg/ml and control group. Total number of cells per blastocyst was not different in the control and RJ-treated group at 5 mg/ml. However, number of apoptotic cells per blastocyst was higher in the control group than in the RJ-treated group at 5 mg/ml. Expression profile of Bax, and p53 was down-regulated while Bcl-2 was up-regulated in oocytes treated with RJ at 5 and 10 mg/ml compared with the control group. Addition of RJ at concentrations of 5 mg/ml improved embryo production through increasing maturation rate. RJ seems to improve the IVM microenvironment by reducing expression of genes inducing apoptosis, enhancing GSH content, and reducing incidence of apoptosis in blastocysts. |
format | Online Article Text |
id | pubmed-8186877 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Colégio Brasileiro de Reprodução Animal |
record_format | MEDLINE/PubMed |
spelling | pubmed-81868772021-06-11 Oocyte maturation with royal jelly increases embryo development and reduces apoptosis in goats Veshkini, Arash Mohammadi-Sangcheshmeh, Abdollah Ghanem, Nasser Abazari-kia, Amir Hossein Mottaghi, Elmira Kamaledini, Reza Deldar, Hamid Ozturk, Irfan Gastal, Eduardo Leite Anim Reprod Original Article Royal jelly (RJ) was supplemented to goat oocyte in vitro maturation (IVM) medium at three different concentrations (2.5, 5, and 10 mg/ml). Maturation rate, embryo cleavage, and blastocyst rate were recorded. Gene expression of apoptosis-related transcripts was investigated in matured oocytes. Percentage of oocytes that reached MII-stage was increased in RJ-treated groups compared to the control group. Glutathione (GSH) content of mature oocytes was enhanced when RJ was added to IVM medium at any supplementation compared with control. Percentage of cleaved embryos and blastocysts was higher in the RJ-treated groups at a concentration of 5 than in the 2.5 mg/ml and control group. Total number of cells per blastocyst was not different in the control and RJ-treated group at 5 mg/ml. However, number of apoptotic cells per blastocyst was higher in the control group than in the RJ-treated group at 5 mg/ml. Expression profile of Bax, and p53 was down-regulated while Bcl-2 was up-regulated in oocytes treated with RJ at 5 and 10 mg/ml compared with the control group. Addition of RJ at concentrations of 5 mg/ml improved embryo production through increasing maturation rate. RJ seems to improve the IVM microenvironment by reducing expression of genes inducing apoptosis, enhancing GSH content, and reducing incidence of apoptosis in blastocysts. Colégio Brasileiro de Reprodução Animal 2018-08-16 /pmc/articles/PMC8186877/ /pubmed/34122643 http://dx.doi.org/10.21451/1984-3143-2017-AR986 Text en https://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Veshkini, Arash Mohammadi-Sangcheshmeh, Abdollah Ghanem, Nasser Abazari-kia, Amir Hossein Mottaghi, Elmira Kamaledini, Reza Deldar, Hamid Ozturk, Irfan Gastal, Eduardo Leite Oocyte maturation with royal jelly increases embryo development and reduces apoptosis in goats |
title |
Oocyte maturation with royal jelly increases embryo development and reduces apoptosis in
goats
|
title_full |
Oocyte maturation with royal jelly increases embryo development and reduces apoptosis in
goats
|
title_fullStr |
Oocyte maturation with royal jelly increases embryo development and reduces apoptosis in
goats
|
title_full_unstemmed |
Oocyte maturation with royal jelly increases embryo development and reduces apoptosis in
goats
|
title_short |
Oocyte maturation with royal jelly increases embryo development and reduces apoptosis in
goats
|
title_sort | oocyte maturation with royal jelly increases embryo development and reduces apoptosis in
goats |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8186877/ https://www.ncbi.nlm.nih.gov/pubmed/34122643 http://dx.doi.org/10.21451/1984-3143-2017-AR986 |
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