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Effect of Cytokine Gene Polymorphisms on Eltrombopag Reactivity in Japanese Patients with Immune Thrombocytopenia

BACKGROUND: Immune thrombocytopenic purpura (ITP) is an autoimmune disease characterized by low platelet counts resulting from antiplatelet autoantibodies. Analysis of polymorphisms in cytokine-encoding genes is important for understanding the pathophysiology of ITP and selecting appropriate treatme...

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Autores principales: Nomura, Shosaku, Abe, Misao, Yamaoka, Manabu, Ito, Tomoki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187034/
https://www.ncbi.nlm.nih.gov/pubmed/34113203
http://dx.doi.org/10.2147/JBM.S309680
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author Nomura, Shosaku
Abe, Misao
Yamaoka, Manabu
Ito, Tomoki
author_facet Nomura, Shosaku
Abe, Misao
Yamaoka, Manabu
Ito, Tomoki
author_sort Nomura, Shosaku
collection PubMed
description BACKGROUND: Immune thrombocytopenic purpura (ITP) is an autoimmune disease characterized by low platelet counts resulting from antiplatelet autoantibodies. Analysis of polymorphisms in cytokine-encoding genes is important for understanding the pathophysiology of ITP and selecting appropriate treatments. We investigated associations between polymorphisms in cytokine-encoding genes and responses to therapy in Japanese patients with ITP. METHODS: The participants in this study comprised 153 patients with ITP and 70 healthy controls. We extracted data on sex, age, platelet counts, bleeding symptoms, and therapeutic responses, including those to prednisolone (PSL) and eltrombopag. Genomic DNA was isolated from peripheral blood and polymorphisms in TNF-α, IL-10, TGF-β(1), and IFN-γ genes were analyzed using the PCR-SSP method. RESULTS: Our results showed that the TGF-β(1) +869 C/C genotype might be related to ITP in Japanese patients. The IL-10 −592 C/C and A/A, −819 C/C and T/T, and −1082, −819, −592 ATA/ATA genotypes might be associated with reactivity to PSL. Furthermore, the IL-10 −592 C/A −819 C/T genotypes, IL-10 ACC/ATA genotype, and TGF-β(1) +869 T/T and T/C genotypes might be linked to the response to eltrombopag. CONCLUSION: Our results indicate that analysis of polymorphisms in cytokine-encoding genes could aid in understanding PSL and eltrombopag responsiveness in Japanese patients with ITP.
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spelling pubmed-81870342021-06-09 Effect of Cytokine Gene Polymorphisms on Eltrombopag Reactivity in Japanese Patients with Immune Thrombocytopenia Nomura, Shosaku Abe, Misao Yamaoka, Manabu Ito, Tomoki J Blood Med Original Research BACKGROUND: Immune thrombocytopenic purpura (ITP) is an autoimmune disease characterized by low platelet counts resulting from antiplatelet autoantibodies. Analysis of polymorphisms in cytokine-encoding genes is important for understanding the pathophysiology of ITP and selecting appropriate treatments. We investigated associations between polymorphisms in cytokine-encoding genes and responses to therapy in Japanese patients with ITP. METHODS: The participants in this study comprised 153 patients with ITP and 70 healthy controls. We extracted data on sex, age, platelet counts, bleeding symptoms, and therapeutic responses, including those to prednisolone (PSL) and eltrombopag. Genomic DNA was isolated from peripheral blood and polymorphisms in TNF-α, IL-10, TGF-β(1), and IFN-γ genes were analyzed using the PCR-SSP method. RESULTS: Our results showed that the TGF-β(1) +869 C/C genotype might be related to ITP in Japanese patients. The IL-10 −592 C/C and A/A, −819 C/C and T/T, and −1082, −819, −592 ATA/ATA genotypes might be associated with reactivity to PSL. Furthermore, the IL-10 −592 C/A −819 C/T genotypes, IL-10 ACC/ATA genotype, and TGF-β(1) +869 T/T and T/C genotypes might be linked to the response to eltrombopag. CONCLUSION: Our results indicate that analysis of polymorphisms in cytokine-encoding genes could aid in understanding PSL and eltrombopag responsiveness in Japanese patients with ITP. Dove 2021-06-04 /pmc/articles/PMC8187034/ /pubmed/34113203 http://dx.doi.org/10.2147/JBM.S309680 Text en © 2021 Nomura et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Nomura, Shosaku
Abe, Misao
Yamaoka, Manabu
Ito, Tomoki
Effect of Cytokine Gene Polymorphisms on Eltrombopag Reactivity in Japanese Patients with Immune Thrombocytopenia
title Effect of Cytokine Gene Polymorphisms on Eltrombopag Reactivity in Japanese Patients with Immune Thrombocytopenia
title_full Effect of Cytokine Gene Polymorphisms on Eltrombopag Reactivity in Japanese Patients with Immune Thrombocytopenia
title_fullStr Effect of Cytokine Gene Polymorphisms on Eltrombopag Reactivity in Japanese Patients with Immune Thrombocytopenia
title_full_unstemmed Effect of Cytokine Gene Polymorphisms on Eltrombopag Reactivity in Japanese Patients with Immune Thrombocytopenia
title_short Effect of Cytokine Gene Polymorphisms on Eltrombopag Reactivity in Japanese Patients with Immune Thrombocytopenia
title_sort effect of cytokine gene polymorphisms on eltrombopag reactivity in japanese patients with immune thrombocytopenia
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187034/
https://www.ncbi.nlm.nih.gov/pubmed/34113203
http://dx.doi.org/10.2147/JBM.S309680
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