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2,3-Butanediol synthesis from glucose supplies NADH for elimination of toxic acetate produced during overflow metabolism

Overflow metabolism-caused acetate accumulation is a major problem that restricts industrial applications of various bacteria. 2,3-Butanediol (2,3-BD) synthesis in microorganisms is an ancient metabolic process with unidentified functions. We demonstrate here that acetate increases and then decrease...

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Autores principales: Meng, Wensi, Zhang, Lijie, Cao, Menghao, Zhang, Yongjia, Zhang, Yipeng, Li, Ping, Kang, Zhaoqi, Guo, Shiting, Xu, Ping, Ma, Cuiqing, Gao, Chao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Singapore 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187413/
https://www.ncbi.nlm.nih.gov/pubmed/34103474
http://dx.doi.org/10.1038/s41421-021-00273-2
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author Meng, Wensi
Zhang, Lijie
Cao, Menghao
Zhang, Yongjia
Zhang, Yipeng
Li, Ping
Kang, Zhaoqi
Guo, Shiting
Xu, Ping
Ma, Cuiqing
Gao, Chao
author_facet Meng, Wensi
Zhang, Lijie
Cao, Menghao
Zhang, Yongjia
Zhang, Yipeng
Li, Ping
Kang, Zhaoqi
Guo, Shiting
Xu, Ping
Ma, Cuiqing
Gao, Chao
author_sort Meng, Wensi
collection PubMed
description Overflow metabolism-caused acetate accumulation is a major problem that restricts industrial applications of various bacteria. 2,3-Butanediol (2,3-BD) synthesis in microorganisms is an ancient metabolic process with unidentified functions. We demonstrate here that acetate increases and then decreases during the growth of a bacterium Enterobacter cloacae subsp. dissolvens SDM. Both bifunctional acetaldehyde/ethanol dehydrogenase AdhE-catalyzed ethanol production and acetate-induced 2,3-BD biosynthesis are indispensable for the elimination of acetate generated during overflow metabolism. 2,3-BD biosynthesis from glucose supplies NADH required for acetate elimination via AdhE-catalyzed ethanol production. The coupling strategy involving 2,3-BD biosynthesis and ethanol production is widely distributed in bacteria and is important for toxic acetate elimination. Finally, we realized the co-production of ethanol and acetoin from chitin, the second most abundant natural biopolymer whose catabolism involves inevitable acetate production through the coupling acetate elimination strategy. The synthesis of a non-toxic chemical such as 2,3-BD may be viewed as a unique overflow metabolism with desirable metabolic functions.
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spelling pubmed-81874132021-06-11 2,3-Butanediol synthesis from glucose supplies NADH for elimination of toxic acetate produced during overflow metabolism Meng, Wensi Zhang, Lijie Cao, Menghao Zhang, Yongjia Zhang, Yipeng Li, Ping Kang, Zhaoqi Guo, Shiting Xu, Ping Ma, Cuiqing Gao, Chao Cell Discov Article Overflow metabolism-caused acetate accumulation is a major problem that restricts industrial applications of various bacteria. 2,3-Butanediol (2,3-BD) synthesis in microorganisms is an ancient metabolic process with unidentified functions. We demonstrate here that acetate increases and then decreases during the growth of a bacterium Enterobacter cloacae subsp. dissolvens SDM. Both bifunctional acetaldehyde/ethanol dehydrogenase AdhE-catalyzed ethanol production and acetate-induced 2,3-BD biosynthesis are indispensable for the elimination of acetate generated during overflow metabolism. 2,3-BD biosynthesis from glucose supplies NADH required for acetate elimination via AdhE-catalyzed ethanol production. The coupling strategy involving 2,3-BD biosynthesis and ethanol production is widely distributed in bacteria and is important for toxic acetate elimination. Finally, we realized the co-production of ethanol and acetoin from chitin, the second most abundant natural biopolymer whose catabolism involves inevitable acetate production through the coupling acetate elimination strategy. The synthesis of a non-toxic chemical such as 2,3-BD may be viewed as a unique overflow metabolism with desirable metabolic functions. Springer Singapore 2021-06-08 /pmc/articles/PMC8187413/ /pubmed/34103474 http://dx.doi.org/10.1038/s41421-021-00273-2 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Meng, Wensi
Zhang, Lijie
Cao, Menghao
Zhang, Yongjia
Zhang, Yipeng
Li, Ping
Kang, Zhaoqi
Guo, Shiting
Xu, Ping
Ma, Cuiqing
Gao, Chao
2,3-Butanediol synthesis from glucose supplies NADH for elimination of toxic acetate produced during overflow metabolism
title 2,3-Butanediol synthesis from glucose supplies NADH for elimination of toxic acetate produced during overflow metabolism
title_full 2,3-Butanediol synthesis from glucose supplies NADH for elimination of toxic acetate produced during overflow metabolism
title_fullStr 2,3-Butanediol synthesis from glucose supplies NADH for elimination of toxic acetate produced during overflow metabolism
title_full_unstemmed 2,3-Butanediol synthesis from glucose supplies NADH for elimination of toxic acetate produced during overflow metabolism
title_short 2,3-Butanediol synthesis from glucose supplies NADH for elimination of toxic acetate produced during overflow metabolism
title_sort 2,3-butanediol synthesis from glucose supplies nadh for elimination of toxic acetate produced during overflow metabolism
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187413/
https://www.ncbi.nlm.nih.gov/pubmed/34103474
http://dx.doi.org/10.1038/s41421-021-00273-2
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