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Biomechanical forces enhance directed migration and activation of bone marrow-derived dendritic cells
Mechanical forces are pervasive in the inflammatory site where dendritic cells (DCs) are activated to migrate into draining lymph nodes. For example, fluid shear stress modulates the movement patterns of DCs, including directness and forward migration indices (FMIs), without chemokine effects. Howev...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187447/ https://www.ncbi.nlm.nih.gov/pubmed/34103554 http://dx.doi.org/10.1038/s41598-021-91117-2 |
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author | Kang, Ji-Hun Lee, Hyun Joo Kim, Ok-Hyeon Yun, Yong Ju Seo, Young-Jin Lee, Hyun Jung |
author_facet | Kang, Ji-Hun Lee, Hyun Joo Kim, Ok-Hyeon Yun, Yong Ju Seo, Young-Jin Lee, Hyun Jung |
author_sort | Kang, Ji-Hun |
collection | PubMed |
description | Mechanical forces are pervasive in the inflammatory site where dendritic cells (DCs) are activated to migrate into draining lymph nodes. For example, fluid shear stress modulates the movement patterns of DCs, including directness and forward migration indices (FMIs), without chemokine effects. However, little is known about the effects of biomechanical forces on the activation of DCs. Accordingly, here we fabricated a microfluidics system to assess how biomechanical forces affect the migration and activity of DCs during inflammation. Based on the structure of edema, we proposed and experimentally analyzed a novel concept for a microchip model that mimicked such vascular architecture. The intensity of shear stress generated in our engineered chip was found as 0.2–0.6 dyne/cm(2) by computational simulation; this value corresponded to inflammation in tissues. In this platform, the directness and FMIs of DCs were significantly increased, whereas the migration velocity of DCs was not altered by shear stress, indicating that mechanical stimuli influenced DC migration. Moreover, DCs with shear stress showed increased expression of the DC activation markers MHC class I and CD86 compared with DCs under static conditions. Taken together, these data suggest that the biomechanical forces are important to regulate the migration and activity of DCs. |
format | Online Article Text |
id | pubmed-8187447 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-81874472021-06-09 Biomechanical forces enhance directed migration and activation of bone marrow-derived dendritic cells Kang, Ji-Hun Lee, Hyun Joo Kim, Ok-Hyeon Yun, Yong Ju Seo, Young-Jin Lee, Hyun Jung Sci Rep Article Mechanical forces are pervasive in the inflammatory site where dendritic cells (DCs) are activated to migrate into draining lymph nodes. For example, fluid shear stress modulates the movement patterns of DCs, including directness and forward migration indices (FMIs), without chemokine effects. However, little is known about the effects of biomechanical forces on the activation of DCs. Accordingly, here we fabricated a microfluidics system to assess how biomechanical forces affect the migration and activity of DCs during inflammation. Based on the structure of edema, we proposed and experimentally analyzed a novel concept for a microchip model that mimicked such vascular architecture. The intensity of shear stress generated in our engineered chip was found as 0.2–0.6 dyne/cm(2) by computational simulation; this value corresponded to inflammation in tissues. In this platform, the directness and FMIs of DCs were significantly increased, whereas the migration velocity of DCs was not altered by shear stress, indicating that mechanical stimuli influenced DC migration. Moreover, DCs with shear stress showed increased expression of the DC activation markers MHC class I and CD86 compared with DCs under static conditions. Taken together, these data suggest that the biomechanical forces are important to regulate the migration and activity of DCs. Nature Publishing Group UK 2021-06-08 /pmc/articles/PMC8187447/ /pubmed/34103554 http://dx.doi.org/10.1038/s41598-021-91117-2 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Kang, Ji-Hun Lee, Hyun Joo Kim, Ok-Hyeon Yun, Yong Ju Seo, Young-Jin Lee, Hyun Jung Biomechanical forces enhance directed migration and activation of bone marrow-derived dendritic cells |
title | Biomechanical forces enhance directed migration and activation of bone marrow-derived dendritic cells |
title_full | Biomechanical forces enhance directed migration and activation of bone marrow-derived dendritic cells |
title_fullStr | Biomechanical forces enhance directed migration and activation of bone marrow-derived dendritic cells |
title_full_unstemmed | Biomechanical forces enhance directed migration and activation of bone marrow-derived dendritic cells |
title_short | Biomechanical forces enhance directed migration and activation of bone marrow-derived dendritic cells |
title_sort | biomechanical forces enhance directed migration and activation of bone marrow-derived dendritic cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187447/ https://www.ncbi.nlm.nih.gov/pubmed/34103554 http://dx.doi.org/10.1038/s41598-021-91117-2 |
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