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Heat Shock Protein-70 Levels Are Associated With a State of Oxidative Damage in the Development of Bronchopulmonary Dysplasia

Background: Heat shock protein-70 (Hsp-70) exhibits cytoprotective effects against oxidative stress-induced airway injury. This study aimed to examine Hsp-70 and 8-hydroxy-2′-deoxyguanosine (8-OHdG) from tracheal aspirates (TA) in very low-birth weight (VLBW) preterm infants to predict the developme...

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Autores principales: Hsiao, Chien-Chou, Lee, Cheng-Han, Yang, Rei-Cheng, Chen, Jia-Yuh, Su, Tzu-Cheng, Chang, Yu-Jun, Lin, Ching-Yuang, Tsai, Yi-Giien
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187579/
https://www.ncbi.nlm.nih.gov/pubmed/34123957
http://dx.doi.org/10.3389/fped.2021.616452
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author Hsiao, Chien-Chou
Lee, Cheng-Han
Yang, Rei-Cheng
Chen, Jia-Yuh
Su, Tzu-Cheng
Chang, Yu-Jun
Lin, Ching-Yuang
Tsai, Yi-Giien
author_facet Hsiao, Chien-Chou
Lee, Cheng-Han
Yang, Rei-Cheng
Chen, Jia-Yuh
Su, Tzu-Cheng
Chang, Yu-Jun
Lin, Ching-Yuang
Tsai, Yi-Giien
author_sort Hsiao, Chien-Chou
collection PubMed
description Background: Heat shock protein-70 (Hsp-70) exhibits cytoprotective effects against oxidative stress-induced airway injury. This study aimed to examine Hsp-70 and 8-hydroxy-2′-deoxyguanosine (8-OHdG) from tracheal aspirates (TA) in very low-birth weight (VLBW) preterm infants to predict the development of bronchopulmonary dysplasia (BPD). Methods: This birth cohort study enrolled 109 VLBW preterm infants, including 32 infants who developed BPD. Hsp-70 and 8-OHdG concentrations from TA were measured by immunoassay. The apoptosis of TA epithelial cells obtained on Day 28 after birth was measured using annexin-V staining assay. Results: Hsp-70 and 8-OHdG levels in TA fluid were persistently increased from Day 1 to Day 28 of life in the BPD group. Multiple linear regression analysis demonstrated that BPD was significantly associated with gestational age, respiratory distress syndrome, and TA Hsp-70 and 8-OHdG levels on post-natal Day 28. The TA Hsp-70 level positively correlated with TA 8-OHdG level on the Day 1 (r = 0.47) and Day 28 of life (r = 0.68). Incubation of recombinant Hsp-70 with primary epithelial cells derived from TA of patients decreased hydrogen peroxide-induced epithelial cell death. Conclusions: Hsp-70 levels are associated with a state of oxidative injury in the development of BPD.
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spelling pubmed-81875792021-06-10 Heat Shock Protein-70 Levels Are Associated With a State of Oxidative Damage in the Development of Bronchopulmonary Dysplasia Hsiao, Chien-Chou Lee, Cheng-Han Yang, Rei-Cheng Chen, Jia-Yuh Su, Tzu-Cheng Chang, Yu-Jun Lin, Ching-Yuang Tsai, Yi-Giien Front Pediatr Pediatrics Background: Heat shock protein-70 (Hsp-70) exhibits cytoprotective effects against oxidative stress-induced airway injury. This study aimed to examine Hsp-70 and 8-hydroxy-2′-deoxyguanosine (8-OHdG) from tracheal aspirates (TA) in very low-birth weight (VLBW) preterm infants to predict the development of bronchopulmonary dysplasia (BPD). Methods: This birth cohort study enrolled 109 VLBW preterm infants, including 32 infants who developed BPD. Hsp-70 and 8-OHdG concentrations from TA were measured by immunoassay. The apoptosis of TA epithelial cells obtained on Day 28 after birth was measured using annexin-V staining assay. Results: Hsp-70 and 8-OHdG levels in TA fluid were persistently increased from Day 1 to Day 28 of life in the BPD group. Multiple linear regression analysis demonstrated that BPD was significantly associated with gestational age, respiratory distress syndrome, and TA Hsp-70 and 8-OHdG levels on post-natal Day 28. The TA Hsp-70 level positively correlated with TA 8-OHdG level on the Day 1 (r = 0.47) and Day 28 of life (r = 0.68). Incubation of recombinant Hsp-70 with primary epithelial cells derived from TA of patients decreased hydrogen peroxide-induced epithelial cell death. Conclusions: Hsp-70 levels are associated with a state of oxidative injury in the development of BPD. Frontiers Media S.A. 2021-05-26 /pmc/articles/PMC8187579/ /pubmed/34123957 http://dx.doi.org/10.3389/fped.2021.616452 Text en Copyright © 2021 Hsiao, Lee, Yang, Chen, Su, Chang, Lin and Tsai. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pediatrics
Hsiao, Chien-Chou
Lee, Cheng-Han
Yang, Rei-Cheng
Chen, Jia-Yuh
Su, Tzu-Cheng
Chang, Yu-Jun
Lin, Ching-Yuang
Tsai, Yi-Giien
Heat Shock Protein-70 Levels Are Associated With a State of Oxidative Damage in the Development of Bronchopulmonary Dysplasia
title Heat Shock Protein-70 Levels Are Associated With a State of Oxidative Damage in the Development of Bronchopulmonary Dysplasia
title_full Heat Shock Protein-70 Levels Are Associated With a State of Oxidative Damage in the Development of Bronchopulmonary Dysplasia
title_fullStr Heat Shock Protein-70 Levels Are Associated With a State of Oxidative Damage in the Development of Bronchopulmonary Dysplasia
title_full_unstemmed Heat Shock Protein-70 Levels Are Associated With a State of Oxidative Damage in the Development of Bronchopulmonary Dysplasia
title_short Heat Shock Protein-70 Levels Are Associated With a State of Oxidative Damage in the Development of Bronchopulmonary Dysplasia
title_sort heat shock protein-70 levels are associated with a state of oxidative damage in the development of bronchopulmonary dysplasia
topic Pediatrics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187579/
https://www.ncbi.nlm.nih.gov/pubmed/34123957
http://dx.doi.org/10.3389/fped.2021.616452
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