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Proteomic Analysis of Humoral Immune Components in Bronchoalveolar Lavage of Patients Infected or Colonized by Aspergillus fumigatus

Humoral immune components have been individually studied in the context of interaction of host with Aspergillus fumigatus, a major airborne fungal pathogen. However, a global view of the multitude and complex nature of humoral immune components is needed to bring new insight into host-Aspergillus in...

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Autores principales: Dellière, Sarah, Duchateau, Magalie, Wong, Sarah Sze Wah, Giai Gianetto, Quentin, Guegan, Hélène, Matondo, Mariette, Gangneux, Jean-Pierre, Aimanianda, Vishukumar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187748/
https://www.ncbi.nlm.nih.gov/pubmed/34122441
http://dx.doi.org/10.3389/fimmu.2021.677798
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author Dellière, Sarah
Duchateau, Magalie
Wong, Sarah Sze Wah
Giai Gianetto, Quentin
Guegan, Hélène
Matondo, Mariette
Gangneux, Jean-Pierre
Aimanianda, Vishukumar
author_facet Dellière, Sarah
Duchateau, Magalie
Wong, Sarah Sze Wah
Giai Gianetto, Quentin
Guegan, Hélène
Matondo, Mariette
Gangneux, Jean-Pierre
Aimanianda, Vishukumar
author_sort Dellière, Sarah
collection PubMed
description Humoral immune components have been individually studied in the context of interaction of host with Aspergillus fumigatus, a major airborne fungal pathogen. However, a global view of the multitude and complex nature of humoral immune components is needed to bring new insight into host-Aspergillus interaction. Therefore, we undertook comparative proteomic analysis of the bronchoalveolar lavage fluid collected from individuals infected or colonized with A. fumigatus versus controls, to identify those alveolar humoral components affected upon A. fumigatus infection. Complement proteins C1q, C8 beta-chain, factor-H, ficolin-1, ficolin-2, mannan binding lectin serine peptidase 2, pentraxin-3 and the surfactant protein-D were identified as the major humoral immune components affected by A. fumigatus infection and colonization. Based on this observation, we hypothesize that crosstalk between these humoral components is essential during host-Aspergillus interaction giving new specific leads to study for better understanding the pathogenesis. Furthermore, the affected humoral components could be potential diagnostic markers of A. fumigatus infection or colonization.
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spelling pubmed-81877482021-06-10 Proteomic Analysis of Humoral Immune Components in Bronchoalveolar Lavage of Patients Infected or Colonized by Aspergillus fumigatus Dellière, Sarah Duchateau, Magalie Wong, Sarah Sze Wah Giai Gianetto, Quentin Guegan, Hélène Matondo, Mariette Gangneux, Jean-Pierre Aimanianda, Vishukumar Front Immunol Immunology Humoral immune components have been individually studied in the context of interaction of host with Aspergillus fumigatus, a major airborne fungal pathogen. However, a global view of the multitude and complex nature of humoral immune components is needed to bring new insight into host-Aspergillus interaction. Therefore, we undertook comparative proteomic analysis of the bronchoalveolar lavage fluid collected from individuals infected or colonized with A. fumigatus versus controls, to identify those alveolar humoral components affected upon A. fumigatus infection. Complement proteins C1q, C8 beta-chain, factor-H, ficolin-1, ficolin-2, mannan binding lectin serine peptidase 2, pentraxin-3 and the surfactant protein-D were identified as the major humoral immune components affected by A. fumigatus infection and colonization. Based on this observation, we hypothesize that crosstalk between these humoral components is essential during host-Aspergillus interaction giving new specific leads to study for better understanding the pathogenesis. Furthermore, the affected humoral components could be potential diagnostic markers of A. fumigatus infection or colonization. Frontiers Media S.A. 2021-05-26 /pmc/articles/PMC8187748/ /pubmed/34122441 http://dx.doi.org/10.3389/fimmu.2021.677798 Text en Copyright © 2021 Dellière, Duchateau, Wong, Giai Gianetto, Guegan, Matondo, Gangneux and Aimanianda https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Dellière, Sarah
Duchateau, Magalie
Wong, Sarah Sze Wah
Giai Gianetto, Quentin
Guegan, Hélène
Matondo, Mariette
Gangneux, Jean-Pierre
Aimanianda, Vishukumar
Proteomic Analysis of Humoral Immune Components in Bronchoalveolar Lavage of Patients Infected or Colonized by Aspergillus fumigatus
title Proteomic Analysis of Humoral Immune Components in Bronchoalveolar Lavage of Patients Infected or Colonized by Aspergillus fumigatus
title_full Proteomic Analysis of Humoral Immune Components in Bronchoalveolar Lavage of Patients Infected or Colonized by Aspergillus fumigatus
title_fullStr Proteomic Analysis of Humoral Immune Components in Bronchoalveolar Lavage of Patients Infected or Colonized by Aspergillus fumigatus
title_full_unstemmed Proteomic Analysis of Humoral Immune Components in Bronchoalveolar Lavage of Patients Infected or Colonized by Aspergillus fumigatus
title_short Proteomic Analysis of Humoral Immune Components in Bronchoalveolar Lavage of Patients Infected or Colonized by Aspergillus fumigatus
title_sort proteomic analysis of humoral immune components in bronchoalveolar lavage of patients infected or colonized by aspergillus fumigatus
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187748/
https://www.ncbi.nlm.nih.gov/pubmed/34122441
http://dx.doi.org/10.3389/fimmu.2021.677798
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