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Genetic Differentiation and Mixed Reproductive Strategies in the Northern Corn Leaf Blight Pathogen Setosphaeria turcica From Sweet Corn in Fujian Province, China
The northern corn leaf blight (NCLB) pathogen Setosphaeria turcica (Luttrell) Leonard and Suggs is one of the main biotic constraints on sweet corn (Zea mays L.) yield and quality in Fujian Province, China. Currently, however, there is comparatively little information available regarding the distrib...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187859/ https://www.ncbi.nlm.nih.gov/pubmed/34122358 http://dx.doi.org/10.3389/fmicb.2021.632575 |
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author | Dai, Yuli Gan, Lin Lan, Chengzhong Lu, Xuesong Yang, Xiujuan Gao, Zhimou |
author_facet | Dai, Yuli Gan, Lin Lan, Chengzhong Lu, Xuesong Yang, Xiujuan Gao, Zhimou |
author_sort | Dai, Yuli |
collection | PubMed |
description | The northern corn leaf blight (NCLB) pathogen Setosphaeria turcica (Luttrell) Leonard and Suggs is one of the main biotic constraints on sweet corn (Zea mays L.) yield and quality in Fujian Province, China. Currently, however, there is comparatively little information available regarding the distribution of mating types, population genetics, and reproductive strategies of this pathogen in Fujian. In this study, we investigated the distribution of mating types and population genetics of 117 isolates of S. turcica collected from seven of the main sweet corn-growing regions in Fujian Province, based on multiple polymerase chain reaction analyses using two mating type-specific primer pairs and 11 inter-simple sequence repeat markers. Furthermore, we examined the mode of reproduction of Fujian S. turcica populations. Both MAT1-1 and MAT1-2 mating types were detected throughout all seven sampling locations. The majority of MAT1-2 isolates were detected from Dongyou, Jian’ou, Pingnan, Songxi, and Longyan, whereas a large proportion of the detected MAT1-1 isolates were among those collected from Dongfeng and Nanjing. Furthermore, we detected five shared multi-locus haplotypes among S. turcica isolates from Dongyou, Jian’ou, Pingnan, Nanjing, and Songxi, whereas no shared haplotypes were observed between the Dongfeng (or Longyan) population and these five populations. Pairwise comparisons of the indices Φ(PT) and Nm, and population structure and principal coordinate analyses indicated genetic differentiation between both the regional and the mating type populations of S. turcica in Fujian. The skewed mating type ratio associated with low a haplotypic diversity and evident linkage disequilibrium reveals a mixed reproductive strategy for S. turcica populations in Fujian Province. The findings of this study advance our current understanding of the genetic diversity, population structure, and reproductive strategies of S. turcica populations infecting sweet corn in Fujian Province, and will potentially contribute to further resistance breeding efforts. |
format | Online Article Text |
id | pubmed-8187859 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-81878592021-06-10 Genetic Differentiation and Mixed Reproductive Strategies in the Northern Corn Leaf Blight Pathogen Setosphaeria turcica From Sweet Corn in Fujian Province, China Dai, Yuli Gan, Lin Lan, Chengzhong Lu, Xuesong Yang, Xiujuan Gao, Zhimou Front Microbiol Microbiology The northern corn leaf blight (NCLB) pathogen Setosphaeria turcica (Luttrell) Leonard and Suggs is one of the main biotic constraints on sweet corn (Zea mays L.) yield and quality in Fujian Province, China. Currently, however, there is comparatively little information available regarding the distribution of mating types, population genetics, and reproductive strategies of this pathogen in Fujian. In this study, we investigated the distribution of mating types and population genetics of 117 isolates of S. turcica collected from seven of the main sweet corn-growing regions in Fujian Province, based on multiple polymerase chain reaction analyses using two mating type-specific primer pairs and 11 inter-simple sequence repeat markers. Furthermore, we examined the mode of reproduction of Fujian S. turcica populations. Both MAT1-1 and MAT1-2 mating types were detected throughout all seven sampling locations. The majority of MAT1-2 isolates were detected from Dongyou, Jian’ou, Pingnan, Songxi, and Longyan, whereas a large proportion of the detected MAT1-1 isolates were among those collected from Dongfeng and Nanjing. Furthermore, we detected five shared multi-locus haplotypes among S. turcica isolates from Dongyou, Jian’ou, Pingnan, Nanjing, and Songxi, whereas no shared haplotypes were observed between the Dongfeng (or Longyan) population and these five populations. Pairwise comparisons of the indices Φ(PT) and Nm, and population structure and principal coordinate analyses indicated genetic differentiation between both the regional and the mating type populations of S. turcica in Fujian. The skewed mating type ratio associated with low a haplotypic diversity and evident linkage disequilibrium reveals a mixed reproductive strategy for S. turcica populations in Fujian Province. The findings of this study advance our current understanding of the genetic diversity, population structure, and reproductive strategies of S. turcica populations infecting sweet corn in Fujian Province, and will potentially contribute to further resistance breeding efforts. Frontiers Media S.A. 2021-05-26 /pmc/articles/PMC8187859/ /pubmed/34122358 http://dx.doi.org/10.3389/fmicb.2021.632575 Text en Copyright © 2021 Dai, Gan, Lan, Lu, Yang and Gao. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Dai, Yuli Gan, Lin Lan, Chengzhong Lu, Xuesong Yang, Xiujuan Gao, Zhimou Genetic Differentiation and Mixed Reproductive Strategies in the Northern Corn Leaf Blight Pathogen Setosphaeria turcica From Sweet Corn in Fujian Province, China |
title | Genetic Differentiation and Mixed Reproductive Strategies in the Northern Corn Leaf Blight Pathogen Setosphaeria turcica From Sweet Corn in Fujian Province, China |
title_full | Genetic Differentiation and Mixed Reproductive Strategies in the Northern Corn Leaf Blight Pathogen Setosphaeria turcica From Sweet Corn in Fujian Province, China |
title_fullStr | Genetic Differentiation and Mixed Reproductive Strategies in the Northern Corn Leaf Blight Pathogen Setosphaeria turcica From Sweet Corn in Fujian Province, China |
title_full_unstemmed | Genetic Differentiation and Mixed Reproductive Strategies in the Northern Corn Leaf Blight Pathogen Setosphaeria turcica From Sweet Corn in Fujian Province, China |
title_short | Genetic Differentiation and Mixed Reproductive Strategies in the Northern Corn Leaf Blight Pathogen Setosphaeria turcica From Sweet Corn in Fujian Province, China |
title_sort | genetic differentiation and mixed reproductive strategies in the northern corn leaf blight pathogen setosphaeria turcica from sweet corn in fujian province, china |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187859/ https://www.ncbi.nlm.nih.gov/pubmed/34122358 http://dx.doi.org/10.3389/fmicb.2021.632575 |
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