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A One Health approach to study the circulation of tick-borne pathogens: A preliminary study

Tick-borne pathogens (TBPs) have complex life cycles involving tick vectors and vertebrate hosts. However, there is limited empirical evidence on the zoonotic circulation of TBPs. In this study, we used a One Health approach to study the possible circulation of TBPs in ticks, animals and humans with...

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Autores principales: Banović, Pavle, Díaz-Sánchez, Adrian Alberto, Galon, Clemence, Foucault-Simonin, Angélique, Simin, Verica, Mijatović, Dragana, Papić, Luka, Wu-Chuang, Alejandra, Obregón, Dasiel, Moutailler, Sara, Cabezas-Cruz, Alejandro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8188046/
https://www.ncbi.nlm.nih.gov/pubmed/34141849
http://dx.doi.org/10.1016/j.onehlt.2021.100270
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author Banović, Pavle
Díaz-Sánchez, Adrian Alberto
Galon, Clemence
Foucault-Simonin, Angélique
Simin, Verica
Mijatović, Dragana
Papić, Luka
Wu-Chuang, Alejandra
Obregón, Dasiel
Moutailler, Sara
Cabezas-Cruz, Alejandro
author_facet Banović, Pavle
Díaz-Sánchez, Adrian Alberto
Galon, Clemence
Foucault-Simonin, Angélique
Simin, Verica
Mijatović, Dragana
Papić, Luka
Wu-Chuang, Alejandra
Obregón, Dasiel
Moutailler, Sara
Cabezas-Cruz, Alejandro
author_sort Banović, Pavle
collection PubMed
description Tick-borne pathogens (TBPs) have complex life cycles involving tick vectors and vertebrate hosts. However, there is limited empirical evidence on the zoonotic circulation of TBPs. In this study, we used a One Health approach to study the possible circulation of TBPs in ticks, animals and humans within a rural household in the foothills of the Fruška Gora mountain, northern Serbia. The presence of TBP DNA was assessed using microfluidic PCR (25 bacterial species, 7 parasite species, 5 bacterial genera, 3 parasite genera) in animal, human and tick samples and the presence of tick-borne encephalitis virus (TBEV) RNA was screened for using RT-qPCR on tick samples. In addition, Lyme borreliosis serology was assessed in patients sera. Rhipicephalus sanguineus and Ixodes ricinus ticks were identified on dogs and Haemaphysalis punctata was identified on house walls. Rickettsia helvetica was the most common pathogen detected in pooled R. sanguineus and I. ricinus tick samples, followed by Hepatozoon canis. None of the H. punctata tick samples tested positive for the presence of TBPs. Anaplasma phagocytophilum and Rickettsia monacensis were the most frequent pathogens detected in dogs, followed by Rickettsia felis, whereas Anaplasma bovis was the only pathogen found in one of the goats tested. None of the human blood samples collected from family members tested positive for the presence of TBPs. Although microfluidic PCR did not detect Borrelia sp. in any of the tested tick or blood samples, a family member with a history of Lyme disease was seropositive for Borrelia burgdorferi sensu lato (s.l.). We conclude that, despite the presence of TBPs in tick and vertebrate reservoirs, there is no evidence of infection with TBPs across various components of the epidemiological chain in a rural Fruška Gora household.
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spelling pubmed-81880462021-06-16 A One Health approach to study the circulation of tick-borne pathogens: A preliminary study Banović, Pavle Díaz-Sánchez, Adrian Alberto Galon, Clemence Foucault-Simonin, Angélique Simin, Verica Mijatović, Dragana Papić, Luka Wu-Chuang, Alejandra Obregón, Dasiel Moutailler, Sara Cabezas-Cruz, Alejandro One Health Research Paper Tick-borne pathogens (TBPs) have complex life cycles involving tick vectors and vertebrate hosts. However, there is limited empirical evidence on the zoonotic circulation of TBPs. In this study, we used a One Health approach to study the possible circulation of TBPs in ticks, animals and humans within a rural household in the foothills of the Fruška Gora mountain, northern Serbia. The presence of TBP DNA was assessed using microfluidic PCR (25 bacterial species, 7 parasite species, 5 bacterial genera, 3 parasite genera) in animal, human and tick samples and the presence of tick-borne encephalitis virus (TBEV) RNA was screened for using RT-qPCR on tick samples. In addition, Lyme borreliosis serology was assessed in patients sera. Rhipicephalus sanguineus and Ixodes ricinus ticks were identified on dogs and Haemaphysalis punctata was identified on house walls. Rickettsia helvetica was the most common pathogen detected in pooled R. sanguineus and I. ricinus tick samples, followed by Hepatozoon canis. None of the H. punctata tick samples tested positive for the presence of TBPs. Anaplasma phagocytophilum and Rickettsia monacensis were the most frequent pathogens detected in dogs, followed by Rickettsia felis, whereas Anaplasma bovis was the only pathogen found in one of the goats tested. None of the human blood samples collected from family members tested positive for the presence of TBPs. Although microfluidic PCR did not detect Borrelia sp. in any of the tested tick or blood samples, a family member with a history of Lyme disease was seropositive for Borrelia burgdorferi sensu lato (s.l.). We conclude that, despite the presence of TBPs in tick and vertebrate reservoirs, there is no evidence of infection with TBPs across various components of the epidemiological chain in a rural Fruška Gora household. Elsevier 2021-06-01 /pmc/articles/PMC8188046/ /pubmed/34141849 http://dx.doi.org/10.1016/j.onehlt.2021.100270 Text en © 2021 Published by Elsevier B.V. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Paper
Banović, Pavle
Díaz-Sánchez, Adrian Alberto
Galon, Clemence
Foucault-Simonin, Angélique
Simin, Verica
Mijatović, Dragana
Papić, Luka
Wu-Chuang, Alejandra
Obregón, Dasiel
Moutailler, Sara
Cabezas-Cruz, Alejandro
A One Health approach to study the circulation of tick-borne pathogens: A preliminary study
title A One Health approach to study the circulation of tick-borne pathogens: A preliminary study
title_full A One Health approach to study the circulation of tick-borne pathogens: A preliminary study
title_fullStr A One Health approach to study the circulation of tick-borne pathogens: A preliminary study
title_full_unstemmed A One Health approach to study the circulation of tick-borne pathogens: A preliminary study
title_short A One Health approach to study the circulation of tick-borne pathogens: A preliminary study
title_sort one health approach to study the circulation of tick-borne pathogens: a preliminary study
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8188046/
https://www.ncbi.nlm.nih.gov/pubmed/34141849
http://dx.doi.org/10.1016/j.onehlt.2021.100270
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