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Detection of Extended-Spectrum β-Lactamases among Acinetobacter Baumannii Isolated from Hospitals of Qazvin, Iran
BACKGROUND: Acinetobacter baumannii is a major contributor to nosocomial infections. Extended-spectrum β-lactamase (ESBL)-producing A. baumannii is spreading worldwide. We aimed to determine the frequency of ESBL-encoding genes in clinical isolates of A. baumannii and to access their clonal relation...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Research and Publications Office of Jimma University
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8188086/ https://www.ncbi.nlm.nih.gov/pubmed/34158773 http://dx.doi.org/10.4314/ejhs.v31i2.4 |
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author | Zarabadi-Pour, Mina Peymani, Amir Habibollah-Pourzereshki, Narges Sarookhani, Mohammad Reza Karami, Ali Akbar Javadi, Amir |
author_facet | Zarabadi-Pour, Mina Peymani, Amir Habibollah-Pourzereshki, Narges Sarookhani, Mohammad Reza Karami, Ali Akbar Javadi, Amir |
author_sort | Zarabadi-Pour, Mina |
collection | PubMed |
description | BACKGROUND: Acinetobacter baumannii is a major contributor to nosocomial infections. Extended-spectrum β-lactamase (ESBL)-producing A. baumannii is spreading worldwide. We aimed to determine the frequency of ESBL-encoding genes in clinical isolates of A. baumannii and to access their clonal relationship by repetitive extragenic palindromic-PCR (rep-PCR). METHODS: In this descriptive cross-sectional study, 203 isolates of A. baumannii were collected from Qazvin hospitals. The Identification of isolates was performed by standard laboratory methods. To verify ESBL production, all isolates were screened by disk agar diffusion and confirmed by the combined disk method. Subsequently, ESBL-encoding genes were detected by PCR and sequencing. Possible clonal association of ESBL-producing isolates was evaluated using rep-PCR. RESULTS: Two hundred (98.5%) isolates showed reduced susceptibility to one of the antibiotics used in the ESBL screening test, of which 127 isolates (62.6%) produced ESBL. PCR results showed bla(OXA-1) (20.5%) was the most prevalent gene followed by bla(TEM-1) (20%), bla(GES-1) (15.7%), bla(CTX-M-15) (7.9%), and bla(PER-1) (1.6%). Rep-PCR results revealed that ESBL-producing isolates belonged to clones A (85%), B (13.4%), and C (1.6%). CONCLUSION: Our study showed the significant presence of bla(OXA-1), bla(TEM-1), bla(GES-1), bla(CTX-M-15), and bla(PER-1) genes in ESBL-producing A. baumannii isolates in the studied hospitals. This is the first report on the emergence of bla(OXA-1) gene in these isolates in Iran. The use of comprehensive antimicrobial treatment guidelines based on laboratory data and appropriate infection control interventions are essential. |
format | Online Article Text |
id | pubmed-8188086 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Research and Publications Office of Jimma University |
record_format | MEDLINE/PubMed |
spelling | pubmed-81880862021-06-21 Detection of Extended-Spectrum β-Lactamases among Acinetobacter Baumannii Isolated from Hospitals of Qazvin, Iran Zarabadi-Pour, Mina Peymani, Amir Habibollah-Pourzereshki, Narges Sarookhani, Mohammad Reza Karami, Ali Akbar Javadi, Amir Ethiop J Health Sci Original Article BACKGROUND: Acinetobacter baumannii is a major contributor to nosocomial infections. Extended-spectrum β-lactamase (ESBL)-producing A. baumannii is spreading worldwide. We aimed to determine the frequency of ESBL-encoding genes in clinical isolates of A. baumannii and to access their clonal relationship by repetitive extragenic palindromic-PCR (rep-PCR). METHODS: In this descriptive cross-sectional study, 203 isolates of A. baumannii were collected from Qazvin hospitals. The Identification of isolates was performed by standard laboratory methods. To verify ESBL production, all isolates were screened by disk agar diffusion and confirmed by the combined disk method. Subsequently, ESBL-encoding genes were detected by PCR and sequencing. Possible clonal association of ESBL-producing isolates was evaluated using rep-PCR. RESULTS: Two hundred (98.5%) isolates showed reduced susceptibility to one of the antibiotics used in the ESBL screening test, of which 127 isolates (62.6%) produced ESBL. PCR results showed bla(OXA-1) (20.5%) was the most prevalent gene followed by bla(TEM-1) (20%), bla(GES-1) (15.7%), bla(CTX-M-15) (7.9%), and bla(PER-1) (1.6%). Rep-PCR results revealed that ESBL-producing isolates belonged to clones A (85%), B (13.4%), and C (1.6%). CONCLUSION: Our study showed the significant presence of bla(OXA-1), bla(TEM-1), bla(GES-1), bla(CTX-M-15), and bla(PER-1) genes in ESBL-producing A. baumannii isolates in the studied hospitals. This is the first report on the emergence of bla(OXA-1) gene in these isolates in Iran. The use of comprehensive antimicrobial treatment guidelines based on laboratory data and appropriate infection control interventions are essential. Research and Publications Office of Jimma University 2021-03 /pmc/articles/PMC8188086/ /pubmed/34158773 http://dx.doi.org/10.4314/ejhs.v31i2.4 Text en © 2021 Mina Z., et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Original Article Zarabadi-Pour, Mina Peymani, Amir Habibollah-Pourzereshki, Narges Sarookhani, Mohammad Reza Karami, Ali Akbar Javadi, Amir Detection of Extended-Spectrum β-Lactamases among Acinetobacter Baumannii Isolated from Hospitals of Qazvin, Iran |
title | Detection of Extended-Spectrum β-Lactamases among Acinetobacter Baumannii Isolated from Hospitals of Qazvin, Iran |
title_full | Detection of Extended-Spectrum β-Lactamases among Acinetobacter Baumannii Isolated from Hospitals of Qazvin, Iran |
title_fullStr | Detection of Extended-Spectrum β-Lactamases among Acinetobacter Baumannii Isolated from Hospitals of Qazvin, Iran |
title_full_unstemmed | Detection of Extended-Spectrum β-Lactamases among Acinetobacter Baumannii Isolated from Hospitals of Qazvin, Iran |
title_short | Detection of Extended-Spectrum β-Lactamases among Acinetobacter Baumannii Isolated from Hospitals of Qazvin, Iran |
title_sort | detection of extended-spectrum β-lactamases among acinetobacter baumannii isolated from hospitals of qazvin, iran |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8188086/ https://www.ncbi.nlm.nih.gov/pubmed/34158773 http://dx.doi.org/10.4314/ejhs.v31i2.4 |
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