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Microbiome-encoded bile acid metabolism modulates colonic transit times
Gut motility is regulated by the microbiome via mechanisms that include bile acid metabolism. To localize the effects of microbiome-generated bile acids, we colonized gnotobiotic mice with different synthetic gut bacterial communities that were metabolically phenotyped using a functional in vitro sc...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8188381/ https://www.ncbi.nlm.nih.gov/pubmed/34142026 http://dx.doi.org/10.1016/j.isci.2021.102508 |
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author | Li, Naisi Koester, Sean T. Lachance, Daniel M. Dutta, Moumita Cui, Julia Yue Dey, Neelendu |
author_facet | Li, Naisi Koester, Sean T. Lachance, Daniel M. Dutta, Moumita Cui, Julia Yue Dey, Neelendu |
author_sort | Li, Naisi |
collection | PubMed |
description | Gut motility is regulated by the microbiome via mechanisms that include bile acid metabolism. To localize the effects of microbiome-generated bile acids, we colonized gnotobiotic mice with different synthetic gut bacterial communities that were metabolically phenotyped using a functional in vitro screen. Using two different marker-based assays of gut transit, we inferred that bile acids exert effects on colonic transit. We validated this using an intra-colonic bile acid infusion assay and determined that these effects were dependent upon signaling via the bile acid receptor, TGR5. The intra-colonic bile acid infusion experiments further revealed sex-biased bile acid-specific effects on colonic transit, with lithocholic acid having the largest pro-motility effect. Transcriptional responses of the enteric nervous system (ENS) were stereotypic, regional, and observed in response to different microbiota, their associated bile acid profiles, and even to a single diet ingredient, evidencing exquisite sensitivity of the ENS to environmental perturbations. |
format | Online Article Text |
id | pubmed-8188381 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-81883812021-06-16 Microbiome-encoded bile acid metabolism modulates colonic transit times Li, Naisi Koester, Sean T. Lachance, Daniel M. Dutta, Moumita Cui, Julia Yue Dey, Neelendu iScience Article Gut motility is regulated by the microbiome via mechanisms that include bile acid metabolism. To localize the effects of microbiome-generated bile acids, we colonized gnotobiotic mice with different synthetic gut bacterial communities that were metabolically phenotyped using a functional in vitro screen. Using two different marker-based assays of gut transit, we inferred that bile acids exert effects on colonic transit. We validated this using an intra-colonic bile acid infusion assay and determined that these effects were dependent upon signaling via the bile acid receptor, TGR5. The intra-colonic bile acid infusion experiments further revealed sex-biased bile acid-specific effects on colonic transit, with lithocholic acid having the largest pro-motility effect. Transcriptional responses of the enteric nervous system (ENS) were stereotypic, regional, and observed in response to different microbiota, their associated bile acid profiles, and even to a single diet ingredient, evidencing exquisite sensitivity of the ENS to environmental perturbations. Elsevier 2021-05-05 /pmc/articles/PMC8188381/ /pubmed/34142026 http://dx.doi.org/10.1016/j.isci.2021.102508 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Li, Naisi Koester, Sean T. Lachance, Daniel M. Dutta, Moumita Cui, Julia Yue Dey, Neelendu Microbiome-encoded bile acid metabolism modulates colonic transit times |
title | Microbiome-encoded bile acid metabolism modulates colonic transit times |
title_full | Microbiome-encoded bile acid metabolism modulates colonic transit times |
title_fullStr | Microbiome-encoded bile acid metabolism modulates colonic transit times |
title_full_unstemmed | Microbiome-encoded bile acid metabolism modulates colonic transit times |
title_short | Microbiome-encoded bile acid metabolism modulates colonic transit times |
title_sort | microbiome-encoded bile acid metabolism modulates colonic transit times |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8188381/ https://www.ncbi.nlm.nih.gov/pubmed/34142026 http://dx.doi.org/10.1016/j.isci.2021.102508 |
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