Protein purification and crystallization of HLA-A∗02:01 in complex with SARS-CoV-2 peptides

Understanding T-cell responses requires identifying viral peptides presented by human leukocyte antigens (HLAs). X-ray crystallography can be used to visualize their presentation. This protocol describes the expression, purification, and crystallization of HLA-A∗02:01, one of the most frequent HLA i...

Descripción completa

Detalles Bibliográficos
Autores principales: Chatzileontiadou, Demetra S.M., Szeto, Christopher, Jayasinghe, Dhilshan, Gras, Stephanie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8188458/
https://www.ncbi.nlm.nih.gov/pubmed/34124695
http://dx.doi.org/10.1016/j.xpro.2021.100635
Descripción
Sumario:Understanding T-cell responses requires identifying viral peptides presented by human leukocyte antigens (HLAs). X-ray crystallography can be used to visualize their presentation. This protocol describes the expression, purification, and crystallization of HLA-A∗02:01, one of the most frequent HLA in the global population in complex with peptides derived from the SARS-CoV-2 nucleocapsid protein. This protocol can be applied to different HLA class I molecules bound to other peptides. For complete details on the use and execution of this protocol, please refer to Szeto et al. (2021).

Ejemplares similares