Protein purification and crystallization of HLA-A∗02:01 in complex with SARS-CoV-2 peptides

Understanding T-cell responses requires identifying viral peptides presented by human leukocyte antigens (HLAs). X-ray crystallography can be used to visualize their presentation. This protocol describes the expression, purification, and crystallization of HLA-A∗02:01, one of the most frequent HLA i...

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Autores principales: Chatzileontiadou, Demetra S.M., Szeto, Christopher, Jayasinghe, Dhilshan, Gras, Stephanie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8188458/
https://www.ncbi.nlm.nih.gov/pubmed/34124695
http://dx.doi.org/10.1016/j.xpro.2021.100635
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author Chatzileontiadou, Demetra S.M.
Szeto, Christopher
Jayasinghe, Dhilshan
Gras, Stephanie
author_facet Chatzileontiadou, Demetra S.M.
Szeto, Christopher
Jayasinghe, Dhilshan
Gras, Stephanie
author_sort Chatzileontiadou, Demetra S.M.
collection PubMed
description Understanding T-cell responses requires identifying viral peptides presented by human leukocyte antigens (HLAs). X-ray crystallography can be used to visualize their presentation. This protocol describes the expression, purification, and crystallization of HLA-A∗02:01, one of the most frequent HLA in the global population in complex with peptides derived from the SARS-CoV-2 nucleocapsid protein. This protocol can be applied to different HLA class I molecules bound to other peptides. For complete details on the use and execution of this protocol, please refer to Szeto et al. (2021).
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spelling pubmed-81884582021-06-09 Protein purification and crystallization of HLA-A∗02:01 in complex with SARS-CoV-2 peptides Chatzileontiadou, Demetra S.M. Szeto, Christopher Jayasinghe, Dhilshan Gras, Stephanie STAR Protoc Protocol Understanding T-cell responses requires identifying viral peptides presented by human leukocyte antigens (HLAs). X-ray crystallography can be used to visualize their presentation. This protocol describes the expression, purification, and crystallization of HLA-A∗02:01, one of the most frequent HLA in the global population in complex with peptides derived from the SARS-CoV-2 nucleocapsid protein. This protocol can be applied to different HLA class I molecules bound to other peptides. For complete details on the use and execution of this protocol, please refer to Szeto et al. (2021). Elsevier 2021-06-09 /pmc/articles/PMC8188458/ /pubmed/34124695 http://dx.doi.org/10.1016/j.xpro.2021.100635 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Chatzileontiadou, Demetra S.M.
Szeto, Christopher
Jayasinghe, Dhilshan
Gras, Stephanie
Protein purification and crystallization of HLA-A∗02:01 in complex with SARS-CoV-2 peptides
title Protein purification and crystallization of HLA-A∗02:01 in complex with SARS-CoV-2 peptides
title_full Protein purification and crystallization of HLA-A∗02:01 in complex with SARS-CoV-2 peptides
title_fullStr Protein purification and crystallization of HLA-A∗02:01 in complex with SARS-CoV-2 peptides
title_full_unstemmed Protein purification and crystallization of HLA-A∗02:01 in complex with SARS-CoV-2 peptides
title_short Protein purification and crystallization of HLA-A∗02:01 in complex with SARS-CoV-2 peptides
title_sort protein purification and crystallization of hla-a∗02:01 in complex with sars-cov-2 peptides
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8188458/
https://www.ncbi.nlm.nih.gov/pubmed/34124695
http://dx.doi.org/10.1016/j.xpro.2021.100635
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