Cargando…

MiRNA post-transcriptional modification dynamics in T cell activation

T cell activation leads to extensive changes in the miRNA repertoire. Although overall miRNA expression decreases within a few hours of T cell activation, some individual miRNAs are specifically upregulated. Using next-generation sequencing, we assessed miRNA expression and post-transcriptional modi...

Descripción completa

Detalles Bibliográficos
Autores principales: Rodríguez-Galán, Ana, Dosil, Sara G., Gómez, Manuel José, Fernández-Delgado, Irene, Fernández-Messina, Lola, Sánchez-Cabo, Fátima, Sánchez-Madrid, Francisco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8188497/
https://www.ncbi.nlm.nih.gov/pubmed/34142042
http://dx.doi.org/10.1016/j.isci.2021.102530
_version_ 1783705341557473280
author Rodríguez-Galán, Ana
Dosil, Sara G.
Gómez, Manuel José
Fernández-Delgado, Irene
Fernández-Messina, Lola
Sánchez-Cabo, Fátima
Sánchez-Madrid, Francisco
author_facet Rodríguez-Galán, Ana
Dosil, Sara G.
Gómez, Manuel José
Fernández-Delgado, Irene
Fernández-Messina, Lola
Sánchez-Cabo, Fátima
Sánchez-Madrid, Francisco
author_sort Rodríguez-Galán, Ana
collection PubMed
description T cell activation leads to extensive changes in the miRNA repertoire. Although overall miRNA expression decreases within a few hours of T cell activation, some individual miRNAs are specifically upregulated. Using next-generation sequencing, we assessed miRNA expression and post-transcriptional modification kinetics in human primary CD4+ T cells upon T cell receptor (TCR) or type I interferon stimulation. This analysis identified differential expression of multiple miRNAs not previously linked to T cell activation. Remarkably, upregulated miRNAs showed a higher frequency of 3′ adenylation. TCR stimulation was followed by increased expression of RNA modifying enzymes and the RNA degrading enzymes Dis3L2 and Eri1. In the midst of this adverse environment, 3′ adenylation may serve a protective function that could be exploited to improve miRNA stability for T cell-targeted therapy.
format Online
Article
Text
id pubmed-8188497
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-81884972021-06-16 MiRNA post-transcriptional modification dynamics in T cell activation Rodríguez-Galán, Ana Dosil, Sara G. Gómez, Manuel José Fernández-Delgado, Irene Fernández-Messina, Lola Sánchez-Cabo, Fátima Sánchez-Madrid, Francisco iScience Article T cell activation leads to extensive changes in the miRNA repertoire. Although overall miRNA expression decreases within a few hours of T cell activation, some individual miRNAs are specifically upregulated. Using next-generation sequencing, we assessed miRNA expression and post-transcriptional modification kinetics in human primary CD4+ T cells upon T cell receptor (TCR) or type I interferon stimulation. This analysis identified differential expression of multiple miRNAs not previously linked to T cell activation. Remarkably, upregulated miRNAs showed a higher frequency of 3′ adenylation. TCR stimulation was followed by increased expression of RNA modifying enzymes and the RNA degrading enzymes Dis3L2 and Eri1. In the midst of this adverse environment, 3′ adenylation may serve a protective function that could be exploited to improve miRNA stability for T cell-targeted therapy. Elsevier 2021-05-12 /pmc/articles/PMC8188497/ /pubmed/34142042 http://dx.doi.org/10.1016/j.isci.2021.102530 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Rodríguez-Galán, Ana
Dosil, Sara G.
Gómez, Manuel José
Fernández-Delgado, Irene
Fernández-Messina, Lola
Sánchez-Cabo, Fátima
Sánchez-Madrid, Francisco
MiRNA post-transcriptional modification dynamics in T cell activation
title MiRNA post-transcriptional modification dynamics in T cell activation
title_full MiRNA post-transcriptional modification dynamics in T cell activation
title_fullStr MiRNA post-transcriptional modification dynamics in T cell activation
title_full_unstemmed MiRNA post-transcriptional modification dynamics in T cell activation
title_short MiRNA post-transcriptional modification dynamics in T cell activation
title_sort mirna post-transcriptional modification dynamics in t cell activation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8188497/
https://www.ncbi.nlm.nih.gov/pubmed/34142042
http://dx.doi.org/10.1016/j.isci.2021.102530
work_keys_str_mv AT rodriguezgalanana mirnaposttranscriptionalmodificationdynamicsintcellactivation
AT dosilsarag mirnaposttranscriptionalmodificationdynamicsintcellactivation
AT gomezmanueljose mirnaposttranscriptionalmodificationdynamicsintcellactivation
AT fernandezdelgadoirene mirnaposttranscriptionalmodificationdynamicsintcellactivation
AT fernandezmessinalola mirnaposttranscriptionalmodificationdynamicsintcellactivation
AT sanchezcabofatima mirnaposttranscriptionalmodificationdynamicsintcellactivation
AT sanchezmadridfrancisco mirnaposttranscriptionalmodificationdynamicsintcellactivation