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Porphyrins produced by acneic Cutibacterium acnes strains activate the inflammasome by inducing K(+) leakage
Some Cutibacterium acnes subgroups dominate on healthy skin, whereas others are frequently acne associated. Here we provide mechanistic insights into this difference, using an anaerobic keratinocyte-sebocyte-C. acnes co-culture model. An acneic C. acnes strain as well as its porphyrins activates NRL...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8188554/ https://www.ncbi.nlm.nih.gov/pubmed/34151228 http://dx.doi.org/10.1016/j.isci.2021.102575 |
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author | Spittaels, Karl-Jan van Uytfanghe, Katleen Zouboulis, Christos C. Stove, Christophe Crabbé, Aurélie Coenye, Tom |
author_facet | Spittaels, Karl-Jan van Uytfanghe, Katleen Zouboulis, Christos C. Stove, Christophe Crabbé, Aurélie Coenye, Tom |
author_sort | Spittaels, Karl-Jan |
collection | PubMed |
description | Some Cutibacterium acnes subgroups dominate on healthy skin, whereas others are frequently acne associated. Here we provide mechanistic insights into this difference, using an anaerobic keratinocyte-sebocyte-C. acnes co-culture model. An acneic C. acnes strain as well as its porphyrins activates NRLP3 inflammasome assembly, whereas this was not observed with a non-acneic strain. Low levels of intracellular K(+) in keratinocytes stimulated with extracted porphyrins or infected with the acneic strain were observed, identifying porphyrin-induced K(+) leakage as trigger for inflammasome activation. Using a panel of C. acnes strains, we found that porphyrin production and IL-1β release are correlated and are higher in acneic strains. This demonstrates that the latter produce more porphyrins, which interact with the keratinocyte cell membrane, leading to K(+) leakage, NLRP3 inflammasome activation, and IL-1β release and provides an explanation for the observation that some C. acnes strains are associated with healthy skin, whereas others dominate in acneic skin. |
format | Online Article Text |
id | pubmed-8188554 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-81885542021-06-17 Porphyrins produced by acneic Cutibacterium acnes strains activate the inflammasome by inducing K(+) leakage Spittaels, Karl-Jan van Uytfanghe, Katleen Zouboulis, Christos C. Stove, Christophe Crabbé, Aurélie Coenye, Tom iScience Article Some Cutibacterium acnes subgroups dominate on healthy skin, whereas others are frequently acne associated. Here we provide mechanistic insights into this difference, using an anaerobic keratinocyte-sebocyte-C. acnes co-culture model. An acneic C. acnes strain as well as its porphyrins activates NRLP3 inflammasome assembly, whereas this was not observed with a non-acneic strain. Low levels of intracellular K(+) in keratinocytes stimulated with extracted porphyrins or infected with the acneic strain were observed, identifying porphyrin-induced K(+) leakage as trigger for inflammasome activation. Using a panel of C. acnes strains, we found that porphyrin production and IL-1β release are correlated and are higher in acneic strains. This demonstrates that the latter produce more porphyrins, which interact with the keratinocyte cell membrane, leading to K(+) leakage, NLRP3 inflammasome activation, and IL-1β release and provides an explanation for the observation that some C. acnes strains are associated with healthy skin, whereas others dominate in acneic skin. Elsevier 2021-05-21 /pmc/articles/PMC8188554/ /pubmed/34151228 http://dx.doi.org/10.1016/j.isci.2021.102575 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Spittaels, Karl-Jan van Uytfanghe, Katleen Zouboulis, Christos C. Stove, Christophe Crabbé, Aurélie Coenye, Tom Porphyrins produced by acneic Cutibacterium acnes strains activate the inflammasome by inducing K(+) leakage |
title | Porphyrins produced by acneic Cutibacterium acnes strains activate the inflammasome by inducing K(+) leakage |
title_full | Porphyrins produced by acneic Cutibacterium acnes strains activate the inflammasome by inducing K(+) leakage |
title_fullStr | Porphyrins produced by acneic Cutibacterium acnes strains activate the inflammasome by inducing K(+) leakage |
title_full_unstemmed | Porphyrins produced by acneic Cutibacterium acnes strains activate the inflammasome by inducing K(+) leakage |
title_short | Porphyrins produced by acneic Cutibacterium acnes strains activate the inflammasome by inducing K(+) leakage |
title_sort | porphyrins produced by acneic cutibacterium acnes strains activate the inflammasome by inducing k(+) leakage |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8188554/ https://www.ncbi.nlm.nih.gov/pubmed/34151228 http://dx.doi.org/10.1016/j.isci.2021.102575 |
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