Cargando…

Using the dCas9-KRAB system to repress gene expression in hiPSC-derived NGN2 neurons

We describe a CRISPR inhibition (CRISPRi) protocol to repress endogenous gene expression (e.g., ATP6V1A) in human induced pluripotent stem cell-derived NGN2-induced glutamatergic neurons. CRISPRi enables efficient and precise gene repression of one or multiple target genes via delivering gRNA(s) to...

Descripción completa

Detalles Bibliográficos
Autores principales:	Li, Aiqun, Cartwright, Samuel, Yu, Alex, Ho, Seok-Man, Schrode, Nadine, Deans, P.J. Michael, Matos, Marliette R., Garcia, Meilin Fernandez, Townsley, Kayla G., Zhang, Bin, Brennand, Kristen J.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2021
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8188621/ https://www.ncbi.nlm.nih.gov/pubmed/34151300 http://dx.doi.org/10.1016/j.xpro.2021.100580

Ejemplares similares

New considerations for hiPSC-based models of neuropsychiatric disorders
por: Hoffman, Gabriel E., et al.
Publicado: (2018)

Combining NGN2 programming and dopaminergic patterning for a rapid and efficient generation of hiPSC-derived midbrain neurons
por: Sheta, Razan, et al.
Publicado: (2022)

Evaluating Synthetic Activation and Repression of Neuropsychiatric-Related Genes in hiPSC-Derived NPCs, Neurons, and Astrocytes
por: Ho, Seok-Man, et al.
Publicado: (2017)

The Importance of Non-neuronal Cell Types in hiPSC-Based Disease Modeling and Drug Screening
por: Gonzalez, David M., et al.
Publicado: (2017)

Divergent Levels of Marker Chromosomes in an hiPSC-Based Model of Psychosis
por: TCW, Julia, et al.
Publicado: (2017)

Phenotypic differences in hiPSC NPCs derived from patients with schizophrenia
por: Brennand, K, et al.
Publicado: (2015)

hiPSC-Derived Cells as Models for Drug Discovery
por: Ofir, Rivka
Publicado: (2021)

A mathematical model of hiPSC cardiomyocytes electromechanics
por: Forouzandehmehr, Mohamadamin, et al.
Publicado: (2021)

Characterizing the secretome of licensed hiPSC-derived MSCs
por: Ramos, Yolande F. M., et al.
Publicado: (2022)

Generation of hiPSC-Derived Skeletal Muscle Cells: Exploiting the Potential of Skeletal Muscle-Derived hiPSCs
por: Metzler, Eric, et al.
Publicado: (2022)

Dopaminergic differentiation of schizophrenia hiPSCs
por: Hartley, Brigham J., et al.
Publicado: (2015)

Reprogramming of HUVECs into Induced Pluripotent Stem Cells (HiPSCs), Generation and Characterization of HiPSC-Derived Neurons and Astrocytes
por: Haile, Yohannes, et al.
Publicado: (2015)

Comparison and Optimization of hiPSC Forebrain Cortical Differentiation Protocols
por: Muratore, Christina R., et al.
Publicado: (2014)

The Effect of Uncoated SPIONs on hiPSC-Differentiated Endothelial Cells
por: Salingova, Barbara, et al.
Publicado: (2019)

Considerations in hiPSC-derived cartilage for articular cartilage repair
por: Yamashita, Akihiro, et al.
Publicado: (2018)

Emerging hiPSC Models for Drug Discovery in Neurodegenerative Diseases
por: Trudler, Dorit, et al.
Publicado: (2021)

α-synuclein pathogenesis in hiPSC models of Parkinson’s disease
por: Baena-Montes, Jara M., et al.
Publicado: (2021)

Inhibition of STEP(61) ameliorates deficits in mouse and hiPSC-based schizophrenia models
por: Xu, J, et al.
Publicado: (2018)

Attenuated transcriptional response to pro-inflammatory cytokines in schizophrenia hiPSC-derived neural progenitor cells
por: Bhat, Anjali, et al.
Publicado: (2022)

Transcriptional signatures of schizophrenia in hiPSC-derived NPCs and neurons are concordant with post-mortem adult brains
por: Hoffman, Gabriel E., et al.
Publicado: (2017)

Repression of HIV-1 reactivation mediated by CRISPR/dCas9-KRAB in lymphoid and myeloid cell models
por: da Costa, Lendel Correia, et al.
Publicado: (2022)

Engineering hiPSC-CM and hiPSC-EC laden 3D nanofibrous splenic hydrogel for improving cardiac function through revascularization and remuscularization in infarcted heart
por: Guan, Ge, et al.
Publicado: (2021)

NGN2 mmRNA-Based Transcriptional Programming in Microfluidic Guides hiPSCs Toward Neural Fate With Multiple Identities
por: Tolomeo, Anna Maria, et al.
Publicado: (2021)

Editing the genome of hiPSC with CRISPR/Cas9: disease models
por: Bassett, Andrew R.
Publicado: (2017)

Practical adoption of state-of-the-art hiPSC-cardiomyocyte differentiation techniques
por: Rupert, Cassady E., et al.
Publicado: (2020)

hiPSC-Derived Cardiac Tissue for Disease Modeling and Drug Discovery
por: Li, Junjun, et al.
Publicado: (2020)

Protocol for Tri-culture of hiPSC-Derived Neurons, Astrocytes, and Microglia
por: Ryan, Sean K., et al.
Publicado: (2020)

An in silico hiPSC-Derived Cardiomyocyte Model Built With Genetic Algorithm
por: Akwaboah, Akwasi D., et al.
Publicado: (2021)

Characterization of the epidermal-dermal junction in hiPSC-derived skin organoids
por: Ramovs, Veronika, et al.
Publicado: (2022)

hiPSC-Derived Cells as Models for Drug Discovery 2.0
por: Ofir, Rivka
Publicado: (2023)

Vascularized hiPSC-derived 3D cardiac microtissue on chip
por: Arslan, Ulgu, et al.
Publicado: (2023)

Vascularized hiPSC-derived 3D cardiac microtissue on chip
por: Arslan, Ulgu, et al.
Publicado: (2023)

Contractile Work Contributes to Maturation of Energy Metabolism in hiPSC-Derived Cardiomyocytes
por: Ulmer, Bärbel M., et al.
Publicado: (2018)

hiPSC-Based Model of Prenatal Exposure to Cannabinoids: Effect on Neuronal Differentiation
por: Miranda, Cláudia C., et al.
Publicado: (2020)

All-Optical Electrophysiology in hiPSC-Derived Neurons With Synthetic Voltage Sensors
por: Puppo, Francesca, et al.
Publicado: (2021)

Microscopy-based cellular contractility assay for adult, neonatal, and hiPSC cardiomyocytes
por: Scalzo, Sérgio, et al.
Publicado: (2022)

hiPSC-Derived Schwann Cells Influence Myogenic Differentiation in Neuromuscular Cocultures
por: Hörner, Sarah Janice, et al.
Publicado: (2021)

Large-scale engineering of hiPSC-derived nephron sheets and cryopreservation of their progenitors
por: Wiersma, Loes E., et al.
Publicado: (2022)

Generation of self-assembling cardiac organoids using hiPSC-derived cardiomyocytes
por: Künzel, Karolina, et al.
Publicado: (2022)

An allied reprogramming, selection, expansion and differentiation platform for creating hiPSC on microcarriers
por: Lam, Alan Tin Lun, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_roctusbkg8i8gkc8bdud0360e0