Efficient generation of isogenic primary human myeloid cells using CRISPR-Cas9 ribonucleoproteins

Genome engineering of primary human cells with CRISPR-Cas9 has revolutionized experimental and therapeutic approaches to cell biology, but human myeloid-lineage cells have remained largely genetically intractable. We present a method for the delivery of CRISPR-Cas9 ribonucleoprotein (RNP) complexes...

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Detalles Bibliográficos
Autores principales: Hiatt, Joseph, Cavero, Devin A., McGregor, Michael J., Zheng, Weihao, Budzik, Jonathan M., Roth, Theodore L., Haas, Kelsey M., Wu, David, Rathore, Ujjwal, Meyer-Franke, Anke, Bouzidi, Mohamed S., Shifrut, Eric, Lee, Youjin, Kumar, Vigneshwari Easwar, Dang, Eric V., Gordon, David E., Wojcechowskyj, Jason A., Hultquist, Judd F., Fontaine, Krystal A., Pillai, Satish K., Cox, Jeffery S., Ernst, Joel D., Krogan, Nevan J., Marson, Alexander
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8188731/
https://www.ncbi.nlm.nih.gov/pubmed/33979618
http://dx.doi.org/10.1016/j.celrep.2021.109105

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