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BUTTERFLY: addressing the pooled amplification paradox with unique molecular identifiers in single-cell RNA-seq

The incorporation of unique molecular identifiers (UMIs) in single-cell RNA-seq assays makes possible the identification of duplicated molecules, thereby facilitating the counting of distinct molecules from sequenced reads. However, we show that the naïve removal of duplicates can lead to a bias due...

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Detalles Bibliográficos
Autores principales:	Gustafsson, Johan, Robinson, Jonathan, Nielsen, Jens, Pachter, Lior
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2021
Materias:	Method
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8188791/ https://www.ncbi.nlm.nih.gov/pubmed/34103073 http://dx.doi.org/10.1186/s13059-021-02386-z

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