Naive stem cell blastocyst model captures human embryo lineage segregation

Human naive pluripotent cells can differentiate into extraembryonic trophectoderm and hypoblast. Here we describe a human embryo model (blastoid) generated by self-organization. Brief induction of trophectoderm leads to formation of blastocyst-like structures within 3 days. Blastoids are composed of...

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Detalles Bibliográficos
Autores principales: Yanagida, Ayaka, Spindlow, Daniel, Nichols, Jennifer, Dattani, Anish, Smith, Austin, Guo, Ge
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cell Press 2021
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8189436/
https://www.ncbi.nlm.nih.gov/pubmed/33957081
http://dx.doi.org/10.1016/j.stem.2021.04.031
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author Yanagida, Ayaka
Spindlow, Daniel
Nichols, Jennifer
Dattani, Anish
Smith, Austin
Guo, Ge
author_facet Yanagida, Ayaka
Spindlow, Daniel
Nichols, Jennifer
Dattani, Anish
Smith, Austin
Guo, Ge
author_sort Yanagida, Ayaka
collection PubMed
description Human naive pluripotent cells can differentiate into extraembryonic trophectoderm and hypoblast. Here we describe a human embryo model (blastoid) generated by self-organization. Brief induction of trophectoderm leads to formation of blastocyst-like structures within 3 days. Blastoids are composed of three tissue layers displaying exclusive lineage markers, mimicking the natural blastocyst. Single-cell transcriptome analyses confirm segregation of trophectoderm, hypoblast, and epiblast with high fidelity to the human embryo. This versatile and scalable system provides a robust experimental model for human embryo research.
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spelling pubmed-81894362021-06-17 Naive stem cell blastocyst model captures human embryo lineage segregation Yanagida, Ayaka Spindlow, Daniel Nichols, Jennifer Dattani, Anish Smith, Austin Guo, Ge Cell Stem Cell Brief Report Human naive pluripotent cells can differentiate into extraembryonic trophectoderm and hypoblast. Here we describe a human embryo model (blastoid) generated by self-organization. Brief induction of trophectoderm leads to formation of blastocyst-like structures within 3 days. Blastoids are composed of three tissue layers displaying exclusive lineage markers, mimicking the natural blastocyst. Single-cell transcriptome analyses confirm segregation of trophectoderm, hypoblast, and epiblast with high fidelity to the human embryo. This versatile and scalable system provides a robust experimental model for human embryo research. Cell Press 2021-06-03 /pmc/articles/PMC8189436/ /pubmed/33957081 http://dx.doi.org/10.1016/j.stem.2021.04.031 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
Yanagida, Ayaka
Spindlow, Daniel
Nichols, Jennifer
Dattani, Anish
Smith, Austin
Guo, Ge
Naive stem cell blastocyst model captures human embryo lineage segregation
title Naive stem cell blastocyst model captures human embryo lineage segregation
title_full Naive stem cell blastocyst model captures human embryo lineage segregation
title_fullStr Naive stem cell blastocyst model captures human embryo lineage segregation
title_full_unstemmed Naive stem cell blastocyst model captures human embryo lineage segregation
title_short Naive stem cell blastocyst model captures human embryo lineage segregation
title_sort naive stem cell blastocyst model captures human embryo lineage segregation
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8189436/
https://www.ncbi.nlm.nih.gov/pubmed/33957081
http://dx.doi.org/10.1016/j.stem.2021.04.031
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