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Human naive epiblast cells possess unrestricted lineage potential

Classic embryological experiments have established that the early mouse embryo develops via sequential lineage bifurcations. The first segregated lineage is the trophectoderm, essential for blastocyst formation. Mouse naive epiblast and derivative embryonic stem cells are restricted accordingly from...

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Autores principales: Guo, Ge, Stirparo, Giuliano Giuseppe, Strawbridge, Stanley E., Spindlow, Daniel, Yang, Jian, Clarke, James, Dattani, Anish, Yanagida, Ayaka, Li, Meng Amy, Myers, Sam, Özel, Buse Nurten, Nichols, Jennifer, Smith, Austin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cell Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8189439/
https://www.ncbi.nlm.nih.gov/pubmed/33831366
http://dx.doi.org/10.1016/j.stem.2021.02.025
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author Guo, Ge
Stirparo, Giuliano Giuseppe
Strawbridge, Stanley E.
Spindlow, Daniel
Yang, Jian
Clarke, James
Dattani, Anish
Yanagida, Ayaka
Li, Meng Amy
Myers, Sam
Özel, Buse Nurten
Nichols, Jennifer
Smith, Austin
author_facet Guo, Ge
Stirparo, Giuliano Giuseppe
Strawbridge, Stanley E.
Spindlow, Daniel
Yang, Jian
Clarke, James
Dattani, Anish
Yanagida, Ayaka
Li, Meng Amy
Myers, Sam
Özel, Buse Nurten
Nichols, Jennifer
Smith, Austin
author_sort Guo, Ge
collection PubMed
description Classic embryological experiments have established that the early mouse embryo develops via sequential lineage bifurcations. The first segregated lineage is the trophectoderm, essential for blastocyst formation. Mouse naive epiblast and derivative embryonic stem cells are restricted accordingly from producing trophectoderm. Here we show, in contrast, that human naive embryonic stem cells readily make blastocyst trophectoderm and descendant trophoblast cell types. Trophectoderm was induced rapidly and efficiently by inhibition of ERK/mitogen-activated protein kinase (MAPK) and Nodal signaling. Transcriptome comparison with the human embryo substantiated direct formation of trophectoderm with subsequent differentiation into syncytiotrophoblast, cytotrophoblast, and downstream trophoblast stem cells. During pluripotency progression lineage potential switches from trophectoderm to amnion. Live-cell tracking revealed that epiblast cells in the human blastocyst are also able to produce trophectoderm. Thus, the paradigm of developmental specification coupled to lineage restriction does not apply to humans. Instead, epiblast plasticity and the potential for blastocyst regeneration are retained until implantation.
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spelling pubmed-81894392021-06-17 Human naive epiblast cells possess unrestricted lineage potential Guo, Ge Stirparo, Giuliano Giuseppe Strawbridge, Stanley E. Spindlow, Daniel Yang, Jian Clarke, James Dattani, Anish Yanagida, Ayaka Li, Meng Amy Myers, Sam Özel, Buse Nurten Nichols, Jennifer Smith, Austin Cell Stem Cell Article Classic embryological experiments have established that the early mouse embryo develops via sequential lineage bifurcations. The first segregated lineage is the trophectoderm, essential for blastocyst formation. Mouse naive epiblast and derivative embryonic stem cells are restricted accordingly from producing trophectoderm. Here we show, in contrast, that human naive embryonic stem cells readily make blastocyst trophectoderm and descendant trophoblast cell types. Trophectoderm was induced rapidly and efficiently by inhibition of ERK/mitogen-activated protein kinase (MAPK) and Nodal signaling. Transcriptome comparison with the human embryo substantiated direct formation of trophectoderm with subsequent differentiation into syncytiotrophoblast, cytotrophoblast, and downstream trophoblast stem cells. During pluripotency progression lineage potential switches from trophectoderm to amnion. Live-cell tracking revealed that epiblast cells in the human blastocyst are also able to produce trophectoderm. Thus, the paradigm of developmental specification coupled to lineage restriction does not apply to humans. Instead, epiblast plasticity and the potential for blastocyst regeneration are retained until implantation. Cell Press 2021-06-03 /pmc/articles/PMC8189439/ /pubmed/33831366 http://dx.doi.org/10.1016/j.stem.2021.02.025 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Guo, Ge
Stirparo, Giuliano Giuseppe
Strawbridge, Stanley E.
Spindlow, Daniel
Yang, Jian
Clarke, James
Dattani, Anish
Yanagida, Ayaka
Li, Meng Amy
Myers, Sam
Özel, Buse Nurten
Nichols, Jennifer
Smith, Austin
Human naive epiblast cells possess unrestricted lineage potential
title Human naive epiblast cells possess unrestricted lineage potential
title_full Human naive epiblast cells possess unrestricted lineage potential
title_fullStr Human naive epiblast cells possess unrestricted lineage potential
title_full_unstemmed Human naive epiblast cells possess unrestricted lineage potential
title_short Human naive epiblast cells possess unrestricted lineage potential
title_sort human naive epiblast cells possess unrestricted lineage potential
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8189439/
https://www.ncbi.nlm.nih.gov/pubmed/33831366
http://dx.doi.org/10.1016/j.stem.2021.02.025
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