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Clinical and molecular characteristics of COVID-19 patients with persistent SARS-CoV-2 infection
The characteristics of COVID-19 patients with persistent SARS-CoV-2 infection are not yet well described. Here, we compare the clinical and molecular features of patients with long duration of viral shedding (LDs) with those from patients with short duration patients (SDs), and healthy donors (HDs)....
Autores principales: | , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8190301/ https://www.ncbi.nlm.nih.gov/pubmed/34108465 http://dx.doi.org/10.1038/s41467-021-23621-y |
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author | Yang, Bin Fan, Junpeng Huang, Jia Guo, Ensong Fu, Yu Liu, Si Xiao, Rourou Liu, Chen Lu, Funian Qin, Tianyu He, Chao Wang, Zizhuo Qin, Xu Hu, Dianxing You, Lixin Li, Xi Wang, Tian Wu, Peng Chen, Gang Zhou, Jianfeng Li, Kezhen Sun, Chaoyang |
author_facet | Yang, Bin Fan, Junpeng Huang, Jia Guo, Ensong Fu, Yu Liu, Si Xiao, Rourou Liu, Chen Lu, Funian Qin, Tianyu He, Chao Wang, Zizhuo Qin, Xu Hu, Dianxing You, Lixin Li, Xi Wang, Tian Wu, Peng Chen, Gang Zhou, Jianfeng Li, Kezhen Sun, Chaoyang |
author_sort | Yang, Bin |
collection | PubMed |
description | The characteristics of COVID-19 patients with persistent SARS-CoV-2 infection are not yet well described. Here, we compare the clinical and molecular features of patients with long duration of viral shedding (LDs) with those from patients with short duration patients (SDs), and healthy donors (HDs). We find that several cytokines and chemokines, such as interleukin (IL)-2, tumor necrosis factor (TNF) and lymphotoxin α (LT-α) are present at lower levels in LDs than SDs. Single-cell RNA sequencing shows that natural killer (NK) cells and CD14(+) monocytes are reduced, while regulatory T cells are increased in LDs; moreover, T and NK cells in LDs are less activated than in SDs. Importantly, most cells in LDs show reduced expression of ribosomal protein (RP) genes and related pathways, with this inversed correlation between RP levels and infection duration further validated in 103 independent patients. Our results thus indicate that immunosuppression and low RP expression may be related to the persistence of the viral infection in COVID-19 patients. |
format | Online Article Text |
id | pubmed-8190301 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-81903012021-07-01 Clinical and molecular characteristics of COVID-19 patients with persistent SARS-CoV-2 infection Yang, Bin Fan, Junpeng Huang, Jia Guo, Ensong Fu, Yu Liu, Si Xiao, Rourou Liu, Chen Lu, Funian Qin, Tianyu He, Chao Wang, Zizhuo Qin, Xu Hu, Dianxing You, Lixin Li, Xi Wang, Tian Wu, Peng Chen, Gang Zhou, Jianfeng Li, Kezhen Sun, Chaoyang Nat Commun Article The characteristics of COVID-19 patients with persistent SARS-CoV-2 infection are not yet well described. Here, we compare the clinical and molecular features of patients with long duration of viral shedding (LDs) with those from patients with short duration patients (SDs), and healthy donors (HDs). We find that several cytokines and chemokines, such as interleukin (IL)-2, tumor necrosis factor (TNF) and lymphotoxin α (LT-α) are present at lower levels in LDs than SDs. Single-cell RNA sequencing shows that natural killer (NK) cells and CD14(+) monocytes are reduced, while regulatory T cells are increased in LDs; moreover, T and NK cells in LDs are less activated than in SDs. Importantly, most cells in LDs show reduced expression of ribosomal protein (RP) genes and related pathways, with this inversed correlation between RP levels and infection duration further validated in 103 independent patients. Our results thus indicate that immunosuppression and low RP expression may be related to the persistence of the viral infection in COVID-19 patients. Nature Publishing Group UK 2021-06-09 /pmc/articles/PMC8190301/ /pubmed/34108465 http://dx.doi.org/10.1038/s41467-021-23621-y Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Yang, Bin Fan, Junpeng Huang, Jia Guo, Ensong Fu, Yu Liu, Si Xiao, Rourou Liu, Chen Lu, Funian Qin, Tianyu He, Chao Wang, Zizhuo Qin, Xu Hu, Dianxing You, Lixin Li, Xi Wang, Tian Wu, Peng Chen, Gang Zhou, Jianfeng Li, Kezhen Sun, Chaoyang Clinical and molecular characteristics of COVID-19 patients with persistent SARS-CoV-2 infection |
title | Clinical and molecular characteristics of COVID-19 patients with persistent SARS-CoV-2 infection |
title_full | Clinical and molecular characteristics of COVID-19 patients with persistent SARS-CoV-2 infection |
title_fullStr | Clinical and molecular characteristics of COVID-19 patients with persistent SARS-CoV-2 infection |
title_full_unstemmed | Clinical and molecular characteristics of COVID-19 patients with persistent SARS-CoV-2 infection |
title_short | Clinical and molecular characteristics of COVID-19 patients with persistent SARS-CoV-2 infection |
title_sort | clinical and molecular characteristics of covid-19 patients with persistent sars-cov-2 infection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8190301/ https://www.ncbi.nlm.nih.gov/pubmed/34108465 http://dx.doi.org/10.1038/s41467-021-23621-y |
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