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The Diagnostic and Prognostic Potential of the B-Cell Repertoire in Membranous Nephropathy

Membranous nephropathy (MN), an autoimmune glomerular disease, is one of the most common causes of nephrotic syndrome in adults. In current clinical practice, the diagnosis is dependent on renal tissue biopsy. A new method for diagnosis and prognosis surveillance is urgently needed for patients. In...

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Autores principales: Su, Zuhui, Jin, Yabin, Zhang, Yu, Guan, Zhanwen, Li, Huishi, Chen, Xiangping, Xie, Chao, Zhang, Chuling, Liu, Xiaofen, Li, Peixian, Ye, Peiyi, Zhang, Lifang, Kong, Yaozhong, Luo, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8190383/
https://www.ncbi.nlm.nih.gov/pubmed/34122405
http://dx.doi.org/10.3389/fimmu.2021.635326
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author Su, Zuhui
Jin, Yabin
Zhang, Yu
Guan, Zhanwen
Li, Huishi
Chen, Xiangping
Xie, Chao
Zhang, Chuling
Liu, Xiaofen
Li, Peixian
Ye, Peiyi
Zhang, Lifang
Kong, Yaozhong
Luo, Wei
author_facet Su, Zuhui
Jin, Yabin
Zhang, Yu
Guan, Zhanwen
Li, Huishi
Chen, Xiangping
Xie, Chao
Zhang, Chuling
Liu, Xiaofen
Li, Peixian
Ye, Peiyi
Zhang, Lifang
Kong, Yaozhong
Luo, Wei
author_sort Su, Zuhui
collection PubMed
description Membranous nephropathy (MN), an autoimmune glomerular disease, is one of the most common causes of nephrotic syndrome in adults. In current clinical practice, the diagnosis is dependent on renal tissue biopsy. A new method for diagnosis and prognosis surveillance is urgently needed for patients. In the present study, we recruited 66 MN patients before any treatment and 11 healthy control (HC) and analyzed multiple aspects of the immunoglobulin heavy chain (IGH) repertoire of these samples using high-throughput sequencing. We found that the abnormalities of CDR-H3 length, hydrophobicity, somatic hypermutation (SHM), and germ line index were progressively more prominent in patients with MN, and the frequency of IGHV3-66 in post-therapy patients was significantly lower than that in pre-therapy patients. Moreover, we found that the IGHV3-38 gene was significantly related to PLA2R, which is the most commonly used biomarker. The most important discovery was that several IGHV, IGHD transcripts, CDR-H3 length, and SHM rate in pre-therapy patients had the potential to predict the therapeutic effect. Our study further demonstrated that the IGH repertoire could be a potential biomarker for prognosis prediction of MN. The landscape of circulating B-lymphocyte repertoires sheds new light on the detection and surveillance of MN.
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spelling pubmed-81903832021-06-11 The Diagnostic and Prognostic Potential of the B-Cell Repertoire in Membranous Nephropathy Su, Zuhui Jin, Yabin Zhang, Yu Guan, Zhanwen Li, Huishi Chen, Xiangping Xie, Chao Zhang, Chuling Liu, Xiaofen Li, Peixian Ye, Peiyi Zhang, Lifang Kong, Yaozhong Luo, Wei Front Immunol Immunology Membranous nephropathy (MN), an autoimmune glomerular disease, is one of the most common causes of nephrotic syndrome in adults. In current clinical practice, the diagnosis is dependent on renal tissue biopsy. A new method for diagnosis and prognosis surveillance is urgently needed for patients. In the present study, we recruited 66 MN patients before any treatment and 11 healthy control (HC) and analyzed multiple aspects of the immunoglobulin heavy chain (IGH) repertoire of these samples using high-throughput sequencing. We found that the abnormalities of CDR-H3 length, hydrophobicity, somatic hypermutation (SHM), and germ line index were progressively more prominent in patients with MN, and the frequency of IGHV3-66 in post-therapy patients was significantly lower than that in pre-therapy patients. Moreover, we found that the IGHV3-38 gene was significantly related to PLA2R, which is the most commonly used biomarker. The most important discovery was that several IGHV, IGHD transcripts, CDR-H3 length, and SHM rate in pre-therapy patients had the potential to predict the therapeutic effect. Our study further demonstrated that the IGH repertoire could be a potential biomarker for prognosis prediction of MN. The landscape of circulating B-lymphocyte repertoires sheds new light on the detection and surveillance of MN. Frontiers Media S.A. 2021-05-27 /pmc/articles/PMC8190383/ /pubmed/34122405 http://dx.doi.org/10.3389/fimmu.2021.635326 Text en Copyright © 2021 Su, Jin, Zhang, Guan, Li, Chen, Xie, Zhang, Liu, Li, Ye, Zhang, Kong and Luo https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Su, Zuhui
Jin, Yabin
Zhang, Yu
Guan, Zhanwen
Li, Huishi
Chen, Xiangping
Xie, Chao
Zhang, Chuling
Liu, Xiaofen
Li, Peixian
Ye, Peiyi
Zhang, Lifang
Kong, Yaozhong
Luo, Wei
The Diagnostic and Prognostic Potential of the B-Cell Repertoire in Membranous Nephropathy
title The Diagnostic and Prognostic Potential of the B-Cell Repertoire in Membranous Nephropathy
title_full The Diagnostic and Prognostic Potential of the B-Cell Repertoire in Membranous Nephropathy
title_fullStr The Diagnostic and Prognostic Potential of the B-Cell Repertoire in Membranous Nephropathy
title_full_unstemmed The Diagnostic and Prognostic Potential of the B-Cell Repertoire in Membranous Nephropathy
title_short The Diagnostic and Prognostic Potential of the B-Cell Repertoire in Membranous Nephropathy
title_sort diagnostic and prognostic potential of the b-cell repertoire in membranous nephropathy
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8190383/
https://www.ncbi.nlm.nih.gov/pubmed/34122405
http://dx.doi.org/10.3389/fimmu.2021.635326
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