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The Diagnostic and Prognostic Potential of the B-Cell Repertoire in Membranous Nephropathy
Membranous nephropathy (MN), an autoimmune glomerular disease, is one of the most common causes of nephrotic syndrome in adults. In current clinical practice, the diagnosis is dependent on renal tissue biopsy. A new method for diagnosis and prognosis surveillance is urgently needed for patients. In...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8190383/ https://www.ncbi.nlm.nih.gov/pubmed/34122405 http://dx.doi.org/10.3389/fimmu.2021.635326 |
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author | Su, Zuhui Jin, Yabin Zhang, Yu Guan, Zhanwen Li, Huishi Chen, Xiangping Xie, Chao Zhang, Chuling Liu, Xiaofen Li, Peixian Ye, Peiyi Zhang, Lifang Kong, Yaozhong Luo, Wei |
author_facet | Su, Zuhui Jin, Yabin Zhang, Yu Guan, Zhanwen Li, Huishi Chen, Xiangping Xie, Chao Zhang, Chuling Liu, Xiaofen Li, Peixian Ye, Peiyi Zhang, Lifang Kong, Yaozhong Luo, Wei |
author_sort | Su, Zuhui |
collection | PubMed |
description | Membranous nephropathy (MN), an autoimmune glomerular disease, is one of the most common causes of nephrotic syndrome in adults. In current clinical practice, the diagnosis is dependent on renal tissue biopsy. A new method for diagnosis and prognosis surveillance is urgently needed for patients. In the present study, we recruited 66 MN patients before any treatment and 11 healthy control (HC) and analyzed multiple aspects of the immunoglobulin heavy chain (IGH) repertoire of these samples using high-throughput sequencing. We found that the abnormalities of CDR-H3 length, hydrophobicity, somatic hypermutation (SHM), and germ line index were progressively more prominent in patients with MN, and the frequency of IGHV3-66 in post-therapy patients was significantly lower than that in pre-therapy patients. Moreover, we found that the IGHV3-38 gene was significantly related to PLA2R, which is the most commonly used biomarker. The most important discovery was that several IGHV, IGHD transcripts, CDR-H3 length, and SHM rate in pre-therapy patients had the potential to predict the therapeutic effect. Our study further demonstrated that the IGH repertoire could be a potential biomarker for prognosis prediction of MN. The landscape of circulating B-lymphocyte repertoires sheds new light on the detection and surveillance of MN. |
format | Online Article Text |
id | pubmed-8190383 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-81903832021-06-11 The Diagnostic and Prognostic Potential of the B-Cell Repertoire in Membranous Nephropathy Su, Zuhui Jin, Yabin Zhang, Yu Guan, Zhanwen Li, Huishi Chen, Xiangping Xie, Chao Zhang, Chuling Liu, Xiaofen Li, Peixian Ye, Peiyi Zhang, Lifang Kong, Yaozhong Luo, Wei Front Immunol Immunology Membranous nephropathy (MN), an autoimmune glomerular disease, is one of the most common causes of nephrotic syndrome in adults. In current clinical practice, the diagnosis is dependent on renal tissue biopsy. A new method for diagnosis and prognosis surveillance is urgently needed for patients. In the present study, we recruited 66 MN patients before any treatment and 11 healthy control (HC) and analyzed multiple aspects of the immunoglobulin heavy chain (IGH) repertoire of these samples using high-throughput sequencing. We found that the abnormalities of CDR-H3 length, hydrophobicity, somatic hypermutation (SHM), and germ line index were progressively more prominent in patients with MN, and the frequency of IGHV3-66 in post-therapy patients was significantly lower than that in pre-therapy patients. Moreover, we found that the IGHV3-38 gene was significantly related to PLA2R, which is the most commonly used biomarker. The most important discovery was that several IGHV, IGHD transcripts, CDR-H3 length, and SHM rate in pre-therapy patients had the potential to predict the therapeutic effect. Our study further demonstrated that the IGH repertoire could be a potential biomarker for prognosis prediction of MN. The landscape of circulating B-lymphocyte repertoires sheds new light on the detection and surveillance of MN. Frontiers Media S.A. 2021-05-27 /pmc/articles/PMC8190383/ /pubmed/34122405 http://dx.doi.org/10.3389/fimmu.2021.635326 Text en Copyright © 2021 Su, Jin, Zhang, Guan, Li, Chen, Xie, Zhang, Liu, Li, Ye, Zhang, Kong and Luo https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Su, Zuhui Jin, Yabin Zhang, Yu Guan, Zhanwen Li, Huishi Chen, Xiangping Xie, Chao Zhang, Chuling Liu, Xiaofen Li, Peixian Ye, Peiyi Zhang, Lifang Kong, Yaozhong Luo, Wei The Diagnostic and Prognostic Potential of the B-Cell Repertoire in Membranous Nephropathy |
title | The Diagnostic and Prognostic Potential of the B-Cell Repertoire in Membranous Nephropathy |
title_full | The Diagnostic and Prognostic Potential of the B-Cell Repertoire in Membranous Nephropathy |
title_fullStr | The Diagnostic and Prognostic Potential of the B-Cell Repertoire in Membranous Nephropathy |
title_full_unstemmed | The Diagnostic and Prognostic Potential of the B-Cell Repertoire in Membranous Nephropathy |
title_short | The Diagnostic and Prognostic Potential of the B-Cell Repertoire in Membranous Nephropathy |
title_sort | diagnostic and prognostic potential of the b-cell repertoire in membranous nephropathy |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8190383/ https://www.ncbi.nlm.nih.gov/pubmed/34122405 http://dx.doi.org/10.3389/fimmu.2021.635326 |
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