Cargando…

Firmicutes Levels in the Mouth Reflect the Gut Condition With Respect to Obesity and Early Childhood Caries

The present cross-sectional study investigated whether Firmicutes (F) and Bacteroidetes (B) levels in the mouth reflected the gut condition in obesity and early childhood caries (ECC). Eighty preschoolers (3-5 years) were equally assigned into four groups: 1. obese + ECC, 2. obese + caries-free (CF)...

Descripción completa

Detalles Bibliográficos
Autores principales: Rizzardi, Karina Ferreira, Indiani, Claudia Maria dos Santos Pereira, Mattos-Graner, Renata de Oliveira, de Sousa, Emerson Tavares, Nobre-dos-Santos, Marinês, Parisotto, Thaís Manzano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8190403/
https://www.ncbi.nlm.nih.gov/pubmed/34123864
http://dx.doi.org/10.3389/fcimb.2021.593734
_version_ 1783705680534831104
author Rizzardi, Karina Ferreira
Indiani, Claudia Maria dos Santos Pereira
Mattos-Graner, Renata de Oliveira
de Sousa, Emerson Tavares
Nobre-dos-Santos, Marinês
Parisotto, Thaís Manzano
author_facet Rizzardi, Karina Ferreira
Indiani, Claudia Maria dos Santos Pereira
Mattos-Graner, Renata de Oliveira
de Sousa, Emerson Tavares
Nobre-dos-Santos, Marinês
Parisotto, Thaís Manzano
author_sort Rizzardi, Karina Ferreira
collection PubMed
description The present cross-sectional study investigated whether Firmicutes (F) and Bacteroidetes (B) levels in the mouth reflected the gut condition in obesity and early childhood caries (ECC). Eighty preschoolers (3-5 years) were equally assigned into four groups: 1. obese + ECC, 2. obese + caries-free (CF), 3. eutrophic + ECC, and 4. eutrophic + CF. Nutritional status and ECC were assessed based on the WHO criteria. Dental biofilm and fecal samples were collected for F and B quantification using RT-PCR analysis. Data were evaluated using three-way-ANOVA and Pearson’s correlation (α = 0.05). Regardless of the anatomical location effect (p = 0.22), there were higher values for F in the obese children + ECC compared with those in obese + caries-free (CF) in both mouth and gut (p < 0.05). The correlation for F at these sites was negative in obese children + ECC (r = −0.48; p = 0.03) and positive in obese children + CF (r=0.50; p = 0.03). Bacteroidetes were influenced by ECC (p = 0.03) and the anatomical location (p = 0.00), and the levels tended to be higher in the mouth of the obese children + ECC (p = 0.04). The F/B ratio was higher in the gut and was affected by the anatomical location (p = 0.00). This preliminary study suggested that modulated by ECC, counts of oral Firmicutes reflected corresponding condition in the gut of obese preschoolers. In addition, we first evidenced that the Firmicutes phylum behave differently according to the nutritional status and caries experience and that supragingival biofilm and gut could share levels of similarity.
format Online
Article
Text
id pubmed-8190403
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-81904032021-06-11 Firmicutes Levels in the Mouth Reflect the Gut Condition With Respect to Obesity and Early Childhood Caries Rizzardi, Karina Ferreira Indiani, Claudia Maria dos Santos Pereira Mattos-Graner, Renata de Oliveira de Sousa, Emerson Tavares Nobre-dos-Santos, Marinês Parisotto, Thaís Manzano Front Cell Infect Microbiol Cellular and Infection Microbiology The present cross-sectional study investigated whether Firmicutes (F) and Bacteroidetes (B) levels in the mouth reflected the gut condition in obesity and early childhood caries (ECC). Eighty preschoolers (3-5 years) were equally assigned into four groups: 1. obese + ECC, 2. obese + caries-free (CF), 3. eutrophic + ECC, and 4. eutrophic + CF. Nutritional status and ECC were assessed based on the WHO criteria. Dental biofilm and fecal samples were collected for F and B quantification using RT-PCR analysis. Data were evaluated using three-way-ANOVA and Pearson’s correlation (α = 0.05). Regardless of the anatomical location effect (p = 0.22), there were higher values for F in the obese children + ECC compared with those in obese + caries-free (CF) in both mouth and gut (p < 0.05). The correlation for F at these sites was negative in obese children + ECC (r = −0.48; p = 0.03) and positive in obese children + CF (r=0.50; p = 0.03). Bacteroidetes were influenced by ECC (p = 0.03) and the anatomical location (p = 0.00), and the levels tended to be higher in the mouth of the obese children + ECC (p = 0.04). The F/B ratio was higher in the gut and was affected by the anatomical location (p = 0.00). This preliminary study suggested that modulated by ECC, counts of oral Firmicutes reflected corresponding condition in the gut of obese preschoolers. In addition, we first evidenced that the Firmicutes phylum behave differently according to the nutritional status and caries experience and that supragingival biofilm and gut could share levels of similarity. Frontiers Media S.A. 2021-05-27 /pmc/articles/PMC8190403/ /pubmed/34123864 http://dx.doi.org/10.3389/fcimb.2021.593734 Text en Copyright © 2021 Rizzardi, Indiani, Mattos-Graner, de Sousa, Nobre-dos-Santos and Parisotto https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Rizzardi, Karina Ferreira
Indiani, Claudia Maria dos Santos Pereira
Mattos-Graner, Renata de Oliveira
de Sousa, Emerson Tavares
Nobre-dos-Santos, Marinês
Parisotto, Thaís Manzano
Firmicutes Levels in the Mouth Reflect the Gut Condition With Respect to Obesity and Early Childhood Caries
title Firmicutes Levels in the Mouth Reflect the Gut Condition With Respect to Obesity and Early Childhood Caries
title_full Firmicutes Levels in the Mouth Reflect the Gut Condition With Respect to Obesity and Early Childhood Caries
title_fullStr Firmicutes Levels in the Mouth Reflect the Gut Condition With Respect to Obesity and Early Childhood Caries
title_full_unstemmed Firmicutes Levels in the Mouth Reflect the Gut Condition With Respect to Obesity and Early Childhood Caries
title_short Firmicutes Levels in the Mouth Reflect the Gut Condition With Respect to Obesity and Early Childhood Caries
title_sort firmicutes levels in the mouth reflect the gut condition with respect to obesity and early childhood caries
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8190403/
https://www.ncbi.nlm.nih.gov/pubmed/34123864
http://dx.doi.org/10.3389/fcimb.2021.593734
work_keys_str_mv AT rizzardikarinaferreira firmicuteslevelsinthemouthreflectthegutconditionwithrespecttoobesityandearlychildhoodcaries
AT indianiclaudiamariadossantospereira firmicuteslevelsinthemouthreflectthegutconditionwithrespecttoobesityandearlychildhoodcaries
AT mattosgranerrenatadeoliveira firmicuteslevelsinthemouthreflectthegutconditionwithrespecttoobesityandearlychildhoodcaries
AT desousaemersontavares firmicuteslevelsinthemouthreflectthegutconditionwithrespecttoobesityandearlychildhoodcaries
AT nobredossantosmarines firmicuteslevelsinthemouthreflectthegutconditionwithrespecttoobesityandearlychildhoodcaries
AT parisottothaismanzano firmicuteslevelsinthemouthreflectthegutconditionwithrespecttoobesityandearlychildhoodcaries