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A protocol for transdifferentiation of human cardiac fibroblasts into endothelial cells via activation of innate immunity
Endothelial cells (ECs) have emerged as key pathogenic players in cardiac disease due to their proximity with cardiomyocytes. Induced pluripotent stem cells (iPSCs) have been employed to generate ECs. However, it may be more clinically relevant to transdifferentiate fibroblasts into ECs directly wit...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8190482/ https://www.ncbi.nlm.nih.gov/pubmed/34151292 http://dx.doi.org/10.1016/j.xpro.2021.100556 |
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author | Liu, Chun Medina, Pedro Thomas, Dilip Chen, Ian Y. Sallam, Karim Sayed, Danish Sayed, Nazish |
author_facet | Liu, Chun Medina, Pedro Thomas, Dilip Chen, Ian Y. Sallam, Karim Sayed, Danish Sayed, Nazish |
author_sort | Liu, Chun |
collection | PubMed |
description | Endothelial cells (ECs) have emerged as key pathogenic players in cardiac disease due to their proximity with cardiomyocytes. Induced pluripotent stem cells (iPSCs) have been employed to generate ECs. However, it may be more clinically relevant to transdifferentiate fibroblasts into ECs directly without introducing pluripotent or virally driven transcription factors. Here, we present a protocol that describes the direct conversion of human cardiac fibroblasts into ECs by leveraging the innate immune system. Our protocol produces bona fide human ECs with 95%–98% purity by first passage. For complete details on the use and execution of this protocol, please refer to Liu et al. (2020) and Sayed et al. (2015). |
format | Online Article Text |
id | pubmed-8190482 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-81904822021-06-17 A protocol for transdifferentiation of human cardiac fibroblasts into endothelial cells via activation of innate immunity Liu, Chun Medina, Pedro Thomas, Dilip Chen, Ian Y. Sallam, Karim Sayed, Danish Sayed, Nazish STAR Protoc Protocol Endothelial cells (ECs) have emerged as key pathogenic players in cardiac disease due to their proximity with cardiomyocytes. Induced pluripotent stem cells (iPSCs) have been employed to generate ECs. However, it may be more clinically relevant to transdifferentiate fibroblasts into ECs directly without introducing pluripotent or virally driven transcription factors. Here, we present a protocol that describes the direct conversion of human cardiac fibroblasts into ECs by leveraging the innate immune system. Our protocol produces bona fide human ECs with 95%–98% purity by first passage. For complete details on the use and execution of this protocol, please refer to Liu et al. (2020) and Sayed et al. (2015). Elsevier 2021-06-05 /pmc/articles/PMC8190482/ /pubmed/34151292 http://dx.doi.org/10.1016/j.xpro.2021.100556 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Protocol Liu, Chun Medina, Pedro Thomas, Dilip Chen, Ian Y. Sallam, Karim Sayed, Danish Sayed, Nazish A protocol for transdifferentiation of human cardiac fibroblasts into endothelial cells via activation of innate immunity |
title | A protocol for transdifferentiation of human cardiac fibroblasts into endothelial cells via activation of innate immunity |
title_full | A protocol for transdifferentiation of human cardiac fibroblasts into endothelial cells via activation of innate immunity |
title_fullStr | A protocol for transdifferentiation of human cardiac fibroblasts into endothelial cells via activation of innate immunity |
title_full_unstemmed | A protocol for transdifferentiation of human cardiac fibroblasts into endothelial cells via activation of innate immunity |
title_short | A protocol for transdifferentiation of human cardiac fibroblasts into endothelial cells via activation of innate immunity |
title_sort | protocol for transdifferentiation of human cardiac fibroblasts into endothelial cells via activation of innate immunity |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8190482/ https://www.ncbi.nlm.nih.gov/pubmed/34151292 http://dx.doi.org/10.1016/j.xpro.2021.100556 |
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