Collection and preprocessing of fine needle aspirate patient samples for single cell profiling and data analysis

High cell viability and recovered cell concentration are typical quality control requirements for single-cell processing and quality data. This protocol describes procedures for sampling, live-cell biobanking, preprocessing for single-cell RNA sequencing, and analysis of fine-needle aspiration (FNA)...

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Autores principales: Tastanova, Aizhan, Ramelyte, Egle, Balázs, Zsolt, Menzel, Ulrike, Beisel, Christian, Krauthammer, Michael, Dummer, Reinhard, Levesque, Mitchell Paul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8190505/
https://www.ncbi.nlm.nih.gov/pubmed/34151301
http://dx.doi.org/10.1016/j.xpro.2021.100581
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author Tastanova, Aizhan
Ramelyte, Egle
Balázs, Zsolt
Menzel, Ulrike
Beisel, Christian
Krauthammer, Michael
Dummer, Reinhard
Levesque, Mitchell Paul
author_facet Tastanova, Aizhan
Ramelyte, Egle
Balázs, Zsolt
Menzel, Ulrike
Beisel, Christian
Krauthammer, Michael
Dummer, Reinhard
Levesque, Mitchell Paul
author_sort Tastanova, Aizhan
collection PubMed
description High cell viability and recovered cell concentration are typical quality control requirements for single-cell processing and quality data. This protocol describes procedures for sampling, live-cell biobanking, preprocessing for single-cell RNA sequencing, and analysis of fine-needle aspiration (FNA) samples of the skin. The minimally invasive nature of FNA collection is more accepted by patients and allows for frequent longitudinal sampling, resulting in high-quality single-cell sequencing data that capture cellular heterogeneity in clinical samples.
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spelling pubmed-81905052021-06-17 Collection and preprocessing of fine needle aspirate patient samples for single cell profiling and data analysis Tastanova, Aizhan Ramelyte, Egle Balázs, Zsolt Menzel, Ulrike Beisel, Christian Krauthammer, Michael Dummer, Reinhard Levesque, Mitchell Paul STAR Protoc Protocol High cell viability and recovered cell concentration are typical quality control requirements for single-cell processing and quality data. This protocol describes procedures for sampling, live-cell biobanking, preprocessing for single-cell RNA sequencing, and analysis of fine-needle aspiration (FNA) samples of the skin. The minimally invasive nature of FNA collection is more accepted by patients and allows for frequent longitudinal sampling, resulting in high-quality single-cell sequencing data that capture cellular heterogeneity in clinical samples. Elsevier 2021-06-05 /pmc/articles/PMC8190505/ /pubmed/34151301 http://dx.doi.org/10.1016/j.xpro.2021.100581 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Tastanova, Aizhan
Ramelyte, Egle
Balázs, Zsolt
Menzel, Ulrike
Beisel, Christian
Krauthammer, Michael
Dummer, Reinhard
Levesque, Mitchell Paul
Collection and preprocessing of fine needle aspirate patient samples for single cell profiling and data analysis
title Collection and preprocessing of fine needle aspirate patient samples for single cell profiling and data analysis
title_full Collection and preprocessing of fine needle aspirate patient samples for single cell profiling and data analysis
title_fullStr Collection and preprocessing of fine needle aspirate patient samples for single cell profiling and data analysis
title_full_unstemmed Collection and preprocessing of fine needle aspirate patient samples for single cell profiling and data analysis
title_short Collection and preprocessing of fine needle aspirate patient samples for single cell profiling and data analysis
title_sort collection and preprocessing of fine needle aspirate patient samples for single cell profiling and data analysis
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8190505/
https://www.ncbi.nlm.nih.gov/pubmed/34151301
http://dx.doi.org/10.1016/j.xpro.2021.100581
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